Stability-indicating HPLC-PDA assay for simultaneous determination of paracetamol, thiamine and pyridoxal phosphate in tablet formulations
With the increased number of multi-drug formulations, there is a need to develop new methods for simultaneous determinations of drugs. A precise, accurate and reliable liquid chromatographic method was developed for simultaneous determination of paracetamol, thiamine, and pyridoxal phosphate in phar...
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| Format: | Article |
| Language: | English |
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Sciendo
2019-06-01
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| Series: | Acta Pharmaceutica |
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| Online Access: | https://doi.org/10.2478/acph-2019-0017 |
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| author | Ali Amir Athar Muhammad Makshoof Ahmed Mahmood Nadeem Kashif Murtaza Ghulam Farooq Umar Salman Muhammad |
| author_facet | Ali Amir Athar Muhammad Makshoof Ahmed Mahmood Nadeem Kashif Murtaza Ghulam Farooq Umar Salman Muhammad |
| author_sort | Ali Amir |
| collection | DOAJ |
| description | With the increased number of multi-drug formulations, there is a need to develop new methods for simultaneous determinations of drugs. A precise, accurate and reliable liquid chromatographic method was developed for simultaneous determination of paracetamol, thiamine, and pyridoxal phosphate in pharmaceutical formulations. Separation of analytes was carried out with an Agilent Poroshell C18 column. A mixture of ammonium phosphate buffer (pH = 3.0), acetonitrile and methanol in the ratio of 86:7:7 (V/V/V) was used as the mobile phase pumped at a flow rate of 1.8 mL min−1. Detection of all three components, impurities and degradation products was performed at the selected wavelength of 270 nm. The developed method was validated in terms of linearity, specificity, precision, accuracy, LOD and LOQ as per ICH guidelines. Linearity of the developed method was found in the range 17.5–30 µg mL−1 for thiamine, 35–60 µg mL−1 for pyridoxal phosphate and 87.5–150 µg mL−1 for paracetamol. The coefficient of determination was ≥ 0.9981 for all three analytes. The proposed HPLC method was found to be simple and reliable for the routine simultaneous analysis of paracetamol, thiamine and pyridoxal phosphate in tablet formulations. Complete separation of analytes in the presence of degradation products indicated selectivity of the method. |
| format | Article |
| id | doaj-art-b0adf26ee8d44793b34bc5900a556b36 |
| institution | Kabale University |
| issn | 1846-9558 |
| language | English |
| publishDate | 2019-06-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Acta Pharmaceutica |
| spelling | doaj-art-b0adf26ee8d44793b34bc5900a556b362025-02-02T11:25:09ZengSciendoActa Pharmaceutica1846-95582019-06-0169224925910.2478/acph-2019-0017acph-2019-0017Stability-indicating HPLC-PDA assay for simultaneous determination of paracetamol, thiamine and pyridoxal phosphate in tablet formulationsAli Amir0Athar Muhammad Makshoof1Ahmed Mahmood2Nadeem Kashif3Murtaza Ghulam4Farooq Umar5Salman Muhammad6Institute of Chemistry University of the Punjab, Lahore54590PakistanInstitute of Chemistry University of the Punjab, Lahore54590PakistanInstitute of Chemistry University of the Punjab, Lahore54590PakistanSchazoo Pharmaceutical Laboratories Lahore- Jaranwala Road, SheikhupuraPakistanSchazoo Pharmaceutical Laboratories Lahore- Jaranwala Road, SheikhupuraPakistanInstitute of Chemistry University of the Punjab, Lahore54590PakistanInstitute of Chemistry University of the Punjab, Lahore54590PakistanWith the increased number of multi-drug formulations, there is a need to develop new methods for simultaneous determinations of drugs. A precise, accurate and reliable liquid chromatographic method was developed for simultaneous determination of paracetamol, thiamine, and pyridoxal phosphate in pharmaceutical formulations. Separation of analytes was carried out with an Agilent Poroshell C18 column. A mixture of ammonium phosphate buffer (pH = 3.0), acetonitrile and methanol in the ratio of 86:7:7 (V/V/V) was used as the mobile phase pumped at a flow rate of 1.8 mL min−1. Detection of all three components, impurities and degradation products was performed at the selected wavelength of 270 nm. The developed method was validated in terms of linearity, specificity, precision, accuracy, LOD and LOQ as per ICH guidelines. Linearity of the developed method was found in the range 17.5–30 µg mL−1 for thiamine, 35–60 µg mL−1 for pyridoxal phosphate and 87.5–150 µg mL−1 for paracetamol. The coefficient of determination was ≥ 0.9981 for all three analytes. The proposed HPLC method was found to be simple and reliable for the routine simultaneous analysis of paracetamol, thiamine and pyridoxal phosphate in tablet formulations. Complete separation of analytes in the presence of degradation products indicated selectivity of the method.https://doi.org/10.2478/acph-2019-0017paracetamolthiaminepyridoxine phosphatedegradationhplc-pda |
| spellingShingle | Ali Amir Athar Muhammad Makshoof Ahmed Mahmood Nadeem Kashif Murtaza Ghulam Farooq Umar Salman Muhammad Stability-indicating HPLC-PDA assay for simultaneous determination of paracetamol, thiamine and pyridoxal phosphate in tablet formulations Acta Pharmaceutica paracetamol thiamine pyridoxine phosphate degradation hplc-pda |
| title | Stability-indicating HPLC-PDA assay for simultaneous determination of paracetamol, thiamine and pyridoxal phosphate in tablet formulations |
| title_full | Stability-indicating HPLC-PDA assay for simultaneous determination of paracetamol, thiamine and pyridoxal phosphate in tablet formulations |
| title_fullStr | Stability-indicating HPLC-PDA assay for simultaneous determination of paracetamol, thiamine and pyridoxal phosphate in tablet formulations |
| title_full_unstemmed | Stability-indicating HPLC-PDA assay for simultaneous determination of paracetamol, thiamine and pyridoxal phosphate in tablet formulations |
| title_short | Stability-indicating HPLC-PDA assay for simultaneous determination of paracetamol, thiamine and pyridoxal phosphate in tablet formulations |
| title_sort | stability indicating hplc pda assay for simultaneous determination of paracetamol thiamine and pyridoxal phosphate in tablet formulations |
| topic | paracetamol thiamine pyridoxine phosphate degradation hplc-pda |
| url | https://doi.org/10.2478/acph-2019-0017 |
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