Effect of Vitamin C on In Vitro Maturation of Iraqi She-Camel Oocytes
This study aimed to know the effect of vitamin C on in vitro oocytes maturation of Iraqi she-camel with different techniques collection. Several oocytes collection technique have been used: Three hundred ninety oocytes were collected from 84 ovaries from Afak slaughterhouse within half to one hour o...
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College of Veterinary Medicine, University of Fallujah
2021-06-01
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| Series: | مجلة الانبار للعلوم البيطرية |
| Online Access: | https://www.anbarjvs.edu.iq/effect-of-vitamin-c-on-in-vitro-maturation-of-iraqi-she-camel-oocytes/ |
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| author | Karrar Al-Malikey Dhia Al-Delemi |
| author_facet | Karrar Al-Malikey Dhia Al-Delemi |
| author_sort | Karrar Al-Malikey |
| collection | DOAJ |
| description | This study aimed to know the effect of vitamin C on in vitro oocytes maturation of Iraqi she-camel with different techniques collection. Several oocytes collection technique have been used: Three hundred ninety oocytes were collected from 84 ovaries from Afak slaughterhouse within half to one hour of slaughter animal and transport by cool box contain normal saline 0.9% (20-25C°) to laboratory of Al-Diwaniyah veterinary Hospital within 1-2 hours. After washing the ovaries with normal saline, each 28 ovaries: oocytes collected by one of the following techniques: Aspiration, slicing, and dissection. Oocytes collected from the three techniques counted and graded. Only grades A and B were selected, and undergo in maturation process. and matured in maturation medium (M199-A) and incubated in CO2 incubator at 5% CO2, 38.5 Cº, and 90% humidity for 24h and supplement the media with 0, 25, 50, 100 µg/ml of vit.C for each technique. Aspirated oocytes were cultured the results was expended cumulus cells was 66.66% (66/99) and the appearance first polar body (F.P.B.) was 68.1% (45/66). Maturation medium supplement with 50µg/ml of vit. C higher rate (84% & 81.8 expended cumulus and F.P.B.) than other groups with significant (P<5%). Maturation oocytes by slicing technique were cultured, expended cumulus was 61.4% (59/96) and the appearance F.P.B. 62.7% (37/59). Maturation medium supplement with 50µg/ml of vit. C (79.16 & 73.68% expended cumulus and F.P.B.) higher rate than other groups with significant (P<5%). Maturation oocytes by dissection technique were cultured. expended cumulus was 57.2% (59/103) and the appearance F.P.B. 55.93% (33/59). Maturation medium supplement with 50µg/ml of vit. C higher rate than other groups with significant (P<5%). |
| format | Article |
| id | doaj-art-b0aca96583b24ca1ac5ebcdc0515c69e |
| institution | Kabale University |
| issn | 1999-6527 2707-0603 |
| language | English |
| publishDate | 2021-06-01 |
| publisher | College of Veterinary Medicine, University of Fallujah |
| record_format | Article |
| series | مجلة الانبار للعلوم البيطرية |
| spelling | doaj-art-b0aca96583b24ca1ac5ebcdc0515c69e2025-08-20T03:38:38ZengCollege of Veterinary Medicine, University of Fallujahمجلة الانبار للعلوم البيطرية1999-65272707-06032021-06-0114110.37940/AJVS.2021.14.1.8Effect of Vitamin C on In Vitro Maturation of Iraqi She-Camel OocytesKarrar Al-MalikeyDhia Al-DelemiThis study aimed to know the effect of vitamin C on in vitro oocytes maturation of Iraqi she-camel with different techniques collection. Several oocytes collection technique have been used: Three hundred ninety oocytes were collected from 84 ovaries from Afak slaughterhouse within half to one hour of slaughter animal and transport by cool box contain normal saline 0.9% (20-25C°) to laboratory of Al-Diwaniyah veterinary Hospital within 1-2 hours. After washing the ovaries with normal saline, each 28 ovaries: oocytes collected by one of the following techniques: Aspiration, slicing, and dissection. Oocytes collected from the three techniques counted and graded. Only grades A and B were selected, and undergo in maturation process. and matured in maturation medium (M199-A) and incubated in CO2 incubator at 5% CO2, 38.5 Cº, and 90% humidity for 24h and supplement the media with 0, 25, 50, 100 µg/ml of vit.C for each technique. Aspirated oocytes were cultured the results was expended cumulus cells was 66.66% (66/99) and the appearance first polar body (F.P.B.) was 68.1% (45/66). Maturation medium supplement with 50µg/ml of vit. C higher rate (84% & 81.8 expended cumulus and F.P.B.) than other groups with significant (P<5%). Maturation oocytes by slicing technique were cultured, expended cumulus was 61.4% (59/96) and the appearance F.P.B. 62.7% (37/59). Maturation medium supplement with 50µg/ml of vit. C (79.16 & 73.68% expended cumulus and F.P.B.) higher rate than other groups with significant (P<5%). Maturation oocytes by dissection technique were cultured. expended cumulus was 57.2% (59/103) and the appearance F.P.B. 55.93% (33/59). Maturation medium supplement with 50µg/ml of vit. C higher rate than other groups with significant (P<5%).https://www.anbarjvs.edu.iq/effect-of-vitamin-c-on-in-vitro-maturation-of-iraqi-she-camel-oocytes/ |
| spellingShingle | Karrar Al-Malikey Dhia Al-Delemi Effect of Vitamin C on In Vitro Maturation of Iraqi She-Camel Oocytes مجلة الانبار للعلوم البيطرية |
| title | Effect of Vitamin C on In Vitro Maturation of Iraqi She-Camel Oocytes |
| title_full | Effect of Vitamin C on In Vitro Maturation of Iraqi She-Camel Oocytes |
| title_fullStr | Effect of Vitamin C on In Vitro Maturation of Iraqi She-Camel Oocytes |
| title_full_unstemmed | Effect of Vitamin C on In Vitro Maturation of Iraqi She-Camel Oocytes |
| title_short | Effect of Vitamin C on In Vitro Maturation of Iraqi She-Camel Oocytes |
| title_sort | effect of vitamin c on in vitro maturation of iraqi she camel oocytes |
| url | https://www.anbarjvs.edu.iq/effect-of-vitamin-c-on-in-vitro-maturation-of-iraqi-she-camel-oocytes/ |
| work_keys_str_mv | AT karraralmalikey effectofvitaminconinvitromaturationofiraqishecameloocytes AT dhiaaldelemi effectofvitaminconinvitromaturationofiraqishecameloocytes |