Study On The Extraction Of Ganoderma Lucidum Polysaccharides By Response Surface Analysis Two-phase Aqueous Extraction
In this paper, the extraction and separation of Ganoderma lucidum polysaccharide was carried out in the aqueous system of polyethylene glycol/ammonium sulfate for the first time. Using ganoderma lucidum mycelium as raw material, the effect of the concentration of phase forming substance on the parti...
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| Format: | Article |
| Language: | English |
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EDP Sciences
2025-01-01
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| Series: | BIO Web of Conferences |
| Online Access: | https://www.bio-conferences.org/articles/bioconf/pdf/2025/33/bioconf_icfsb2025_01010.pdf |
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| author | Zhang Yinglong Zhang Zhe Xu Yuanxi Ding Xinli Wu Jiang Jiang Haifang |
| author_facet | Zhang Yinglong Zhang Zhe Xu Yuanxi Ding Xinli Wu Jiang Jiang Haifang |
| author_sort | Zhang Yinglong |
| collection | DOAJ |
| description | In this paper, the extraction and separation of Ganoderma lucidum polysaccharide was carried out in the aqueous system of polyethylene glycol/ammonium sulfate for the first time. Using ganoderma lucidum mycelium as raw material, the effect of the concentration of phase forming substance on the partition coefficient of Ganoderma lucidum polysaccharide in the two-phase aqueous system was studied, and the lateral comparison was made with the traditional alcohol precipitation and hot water extraction of Ganoderma lucidum polysaccharide. The optimal ratio of polyethylene glycol and ammonium sulfate in the two-phase aqueous system was determined by the response surface design optimization experiment. The results show that when pH is 6.5, PEG6000 concentration is 16%, and ammonium sulfate concentration is 35%, the partition coefficient K of polysaccharide can reach 1.8-2.1. The study of this method provides a new method and idea for the extraction of Ganoderma lucidum polysaccharide in the future. |
| format | Article |
| id | doaj-art-b09d7343bcb84d6ab4a993e6d0df3361 |
| institution | Kabale University |
| issn | 2117-4458 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | EDP Sciences |
| record_format | Article |
| series | BIO Web of Conferences |
| spelling | doaj-art-b09d7343bcb84d6ab4a993e6d0df33612025-08-20T03:31:44ZengEDP SciencesBIO Web of Conferences2117-44582025-01-011820101010.1051/bioconf/202518201010bioconf_icfsb2025_01010Study On The Extraction Of Ganoderma Lucidum Polysaccharides By Response Surface Analysis Two-phase Aqueous ExtractionZhang Yinglong0Zhang Zhe1Xu Yuanxi2Ding Xinli3Wu Jiang4Jiang Haifang5Shandong Vocational College of CommerceFangyuan Mark Inspection (Shandong) Co., LTD.Shandong Vocational College of CommerceShandong Vocational College of CommerceShandong Vocational College of CommerceShandong Vocational College of CommerceIn this paper, the extraction and separation of Ganoderma lucidum polysaccharide was carried out in the aqueous system of polyethylene glycol/ammonium sulfate for the first time. Using ganoderma lucidum mycelium as raw material, the effect of the concentration of phase forming substance on the partition coefficient of Ganoderma lucidum polysaccharide in the two-phase aqueous system was studied, and the lateral comparison was made with the traditional alcohol precipitation and hot water extraction of Ganoderma lucidum polysaccharide. The optimal ratio of polyethylene glycol and ammonium sulfate in the two-phase aqueous system was determined by the response surface design optimization experiment. The results show that when pH is 6.5, PEG6000 concentration is 16%, and ammonium sulfate concentration is 35%, the partition coefficient K of polysaccharide can reach 1.8-2.1. The study of this method provides a new method and idea for the extraction of Ganoderma lucidum polysaccharide in the future.https://www.bio-conferences.org/articles/bioconf/pdf/2025/33/bioconf_icfsb2025_01010.pdf |
| spellingShingle | Zhang Yinglong Zhang Zhe Xu Yuanxi Ding Xinli Wu Jiang Jiang Haifang Study On The Extraction Of Ganoderma Lucidum Polysaccharides By Response Surface Analysis Two-phase Aqueous Extraction BIO Web of Conferences |
| title | Study On The Extraction Of Ganoderma Lucidum Polysaccharides By Response Surface Analysis Two-phase Aqueous Extraction |
| title_full | Study On The Extraction Of Ganoderma Lucidum Polysaccharides By Response Surface Analysis Two-phase Aqueous Extraction |
| title_fullStr | Study On The Extraction Of Ganoderma Lucidum Polysaccharides By Response Surface Analysis Two-phase Aqueous Extraction |
| title_full_unstemmed | Study On The Extraction Of Ganoderma Lucidum Polysaccharides By Response Surface Analysis Two-phase Aqueous Extraction |
| title_short | Study On The Extraction Of Ganoderma Lucidum Polysaccharides By Response Surface Analysis Two-phase Aqueous Extraction |
| title_sort | study on the extraction of ganoderma lucidum polysaccharides by response surface analysis two phase aqueous extraction |
| url | https://www.bio-conferences.org/articles/bioconf/pdf/2025/33/bioconf_icfsb2025_01010.pdf |
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