Peptides Derived from Mycobacterium leprae ML1601c Discriminate between Leprosy Patients and Healthy Endemic Controls
The stable incidence of new leprosy cases suggests that transmission of infection continues despite worldwide implementation of MDT. Thus, specific tools are needed to diagnose early stage Mycobacterium leprae infection, the likely sources of transmission. M. leprae antigens that induce T-cell respo...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | Journal of Tropical Medicine |
| Online Access: | http://dx.doi.org/10.1155/2012/132049 |
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| author | Kidist Bobosha Jolien J. van der Ploeg-van Schip Danuza A. Esquenazi Marjorie M. Guimarães Marcia V. Martins Yonas Bekele Yonas Fantahun Abraham Aseffa Kees L. M. C. Franken Ronaldo C. Gismondi Maria C. V. Pessolani Tom H. M. Ottenhoff Geraldo M. B. Pereira Annemieke Geluk |
| author_facet | Kidist Bobosha Jolien J. van der Ploeg-van Schip Danuza A. Esquenazi Marjorie M. Guimarães Marcia V. Martins Yonas Bekele Yonas Fantahun Abraham Aseffa Kees L. M. C. Franken Ronaldo C. Gismondi Maria C. V. Pessolani Tom H. M. Ottenhoff Geraldo M. B. Pereira Annemieke Geluk |
| author_sort | Kidist Bobosha |
| collection | DOAJ |
| description | The stable incidence of new leprosy cases suggests that transmission of infection continues despite worldwide implementation of MDT. Thus, specific tools are needed to diagnose early stage Mycobacterium leprae infection, the likely sources of transmission. M. leprae antigens that induce T-cell responses in M. leprae exposed and/or infected individuals thus are major targets for new diagnostic tools. Previously, we showed that ML1601c was immunogenic in patients and healthy household contacts (HHC). However, some endemic controls (EC) also recognized this protein. To improve the diagnostic potential, IFN-γ responses to ML1601c peptides were assessed using PBMC from Brazilian leprosy patients and EC. Five ML1601c peptides only induced IFN-γ in patients and HHC. Moreover, 24-hour whole-blood assay (WBA), two ML1601c peptides could assess the level of M. leprae exposure in Ethiopian EC. Beside IFN-γ, also IP-10, IL-6, IL-1β, TNF-α, and MCP-1 were increased in EC from areas with high leprosy prevalence in response to these ML1601c peptides. Thus, ML1601c peptides may be useful for differentiating M. leprae exposed or infected individuals and can also be used to indicate the magnitude of M. leprae transmission even in the context of various HLA alleles as present in these different genetic backgrounds. |
| format | Article |
| id | doaj-art-b09d094e5f5b4b58936cfde2cd417bd1 |
| institution | OA Journals |
| issn | 1687-9686 1687-9694 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Tropical Medicine |
| spelling | doaj-art-b09d094e5f5b4b58936cfde2cd417bd12025-08-20T02:19:18ZengWileyJournal of Tropical Medicine1687-96861687-96942012-01-01201210.1155/2012/132049132049Peptides Derived from Mycobacterium leprae ML1601c Discriminate between Leprosy Patients and Healthy Endemic ControlsKidist Bobosha0Jolien J. van der Ploeg-van Schip1Danuza A. Esquenazi2Marjorie M. Guimarães3Marcia V. Martins4Yonas Bekele5Yonas Fantahun6Abraham Aseffa7Kees L. M. C. Franken8Ronaldo C. Gismondi9Maria C. V. Pessolani10Tom H. M. Ottenhoff11Geraldo M. B. Pereira12Annemieke Geluk13Department of Infectious Diseases, LUMC, P.O. Box 9600, 2300 RC Leiden, The NetherlandsDepartment of Infectious Diseases, LUMC, P.O. Box 9600, 2300 RC Leiden, The NetherlandsThe Laboratory of Cellular Microbiology, Oswaldo Cruz Institute, FIOCRUZ, 21040-360 Rio de Janeiro, RJ, BrazilThe Laboratory of Cellular Microbiology, Oswaldo Cruz Institute, FIOCRUZ, 21040-360 Rio de Janeiro, RJ, BrazilThe Laboratory of Cellular Microbiology, Oswaldo Cruz Institute, FIOCRUZ, 21040-360 Rio de Janeiro, RJ, BrazilLeprosy section, Armauer Hansen Research Institute, P.O. Box 1005, Addis Ababa, EthiopiaLeprosy section, Armauer Hansen Research Institute, P.O. Box 1005, Addis Ababa, EthiopiaLeprosy section, Armauer Hansen Research Institute, P.O. Box 1005, Addis Ababa, EthiopiaDepartment of Infectious Diseases, LUMC, P.O. Box 9600, 2300 RC Leiden, The NetherlandsSchool of Medical Sciences, State University of Rio de Janeiro, 20550-170 Rio de Janeiro, RJ, BrazilThe Laboratory of Cellular Microbiology, Oswaldo Cruz Institute, FIOCRUZ, 21040-360 Rio de Janeiro, RJ, BrazilDepartment of Infectious Diseases, LUMC, P.O. Box 9600, 2300 RC Leiden, The NetherlandsThe Laboratory of Cellular Microbiology, Oswaldo Cruz Institute, FIOCRUZ, 21040-360 Rio de Janeiro, RJ, BrazilDepartment of Infectious Diseases, LUMC, P.O. Box 9600, 2300 RC Leiden, The NetherlandsThe stable incidence of new leprosy cases suggests that transmission of infection continues despite worldwide implementation of MDT. Thus, specific tools are needed to diagnose early stage Mycobacterium leprae infection, the likely sources of transmission. M. leprae antigens that induce T-cell responses in M. leprae exposed and/or infected individuals thus are major targets for new diagnostic tools. Previously, we showed that ML1601c was immunogenic in patients and healthy household contacts (HHC). However, some endemic controls (EC) also recognized this protein. To improve the diagnostic potential, IFN-γ responses to ML1601c peptides were assessed using PBMC from Brazilian leprosy patients and EC. Five ML1601c peptides only induced IFN-γ in patients and HHC. Moreover, 24-hour whole-blood assay (WBA), two ML1601c peptides could assess the level of M. leprae exposure in Ethiopian EC. Beside IFN-γ, also IP-10, IL-6, IL-1β, TNF-α, and MCP-1 were increased in EC from areas with high leprosy prevalence in response to these ML1601c peptides. Thus, ML1601c peptides may be useful for differentiating M. leprae exposed or infected individuals and can also be used to indicate the magnitude of M. leprae transmission even in the context of various HLA alleles as present in these different genetic backgrounds.http://dx.doi.org/10.1155/2012/132049 |
| spellingShingle | Kidist Bobosha Jolien J. van der Ploeg-van Schip Danuza A. Esquenazi Marjorie M. Guimarães Marcia V. Martins Yonas Bekele Yonas Fantahun Abraham Aseffa Kees L. M. C. Franken Ronaldo C. Gismondi Maria C. V. Pessolani Tom H. M. Ottenhoff Geraldo M. B. Pereira Annemieke Geluk Peptides Derived from Mycobacterium leprae ML1601c Discriminate between Leprosy Patients and Healthy Endemic Controls Journal of Tropical Medicine |
| title | Peptides Derived from Mycobacterium leprae ML1601c Discriminate between Leprosy Patients and Healthy Endemic Controls |
| title_full | Peptides Derived from Mycobacterium leprae ML1601c Discriminate between Leprosy Patients and Healthy Endemic Controls |
| title_fullStr | Peptides Derived from Mycobacterium leprae ML1601c Discriminate between Leprosy Patients and Healthy Endemic Controls |
| title_full_unstemmed | Peptides Derived from Mycobacterium leprae ML1601c Discriminate between Leprosy Patients and Healthy Endemic Controls |
| title_short | Peptides Derived from Mycobacterium leprae ML1601c Discriminate between Leprosy Patients and Healthy Endemic Controls |
| title_sort | peptides derived from mycobacterium leprae ml1601c discriminate between leprosy patients and healthy endemic controls |
| url | http://dx.doi.org/10.1155/2012/132049 |
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