Cytokine and eicosanoid regulation by Schistosoma mansoniduring LSE penetration
Cercarial penetration, in low to moderate numbers, does not cause a normal skin inflammatory response; therefore, the authors sought to determine whether cercariae can down-regulate keratinocyte activation and thus the secretion of pro-inflammatory cytokines and eicosanoids. Human living skin equiva...
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| Format: | Article |
| Language: | English |
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Wiley
1993-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/S0962935193000109 |
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| _version_ | 1832561728508919808 |
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| author | A. C. Fusco B. Salafsky T. Shibuya |
| author_facet | A. C. Fusco B. Salafsky T. Shibuya |
| author_sort | A. C. Fusco |
| collection | DOAJ |
| description | Cercarial penetration, in low to moderate numbers, does not cause a normal skin inflammatory response; therefore, the authors sought to determine whether cercariae can down-regulate keratinocyte activation and thus the secretion of pro-inflammatory cytokines and eicosanoids. Human living skin equivalent (LSE, Organogenesis) consisting of dermal, epidermal and stratum corneum-like layers was used as the skin substrate. The surface of the LSE membrane was exposed to 100 ng IFNγ or ~850 cercariae for 18 h. Incubation media and tissue was then assayed for IL-1α, IL-6, IL-8, TNFα, 5-HETE, 12-HETE, PGF2, LTB4, and LTC4 via RIA and Western Blots. TNFα was not detected. Secreted IL-1α levels were (mean ± S.E.M. (n)): Control, 1.03 ng ± 0.15 (11); IFNγ 1.90 ng ± 0.48 (5); cercariae, 1.79 ng ± 0.22 (22). In spite of this increase, cercariae down-regulated IL-8 (cercariae 11.13 ± 1.70 ng vs. IFNγ = 16.47 ± 0.29 ng, p = 0.04) and LTB4 (cercariae = 98.86 ± 19.65 pg/0.1 ml vs. IFNγ = 193.42 ± 44.21 pg/0.1 ml p = 0.02). No changes were seen in IL-6, 12-HETE, 5-HETE, and PGE2 levels. It is concluded that cercarial penetration causes a release of IL-1α consistent with skin trauma; however, schistosomulae may regulate the production of chemotactic (neutrophils, macrophages, T-cells, etc.) and activation factors such as IL-8 and LTB4. |
| format | Article |
| id | doaj-art-b08db7370f1445bba9dc29a2ee9ab96b |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 1993-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-b08db7370f1445bba9dc29a2ee9ab96b2025-02-03T01:24:28ZengWileyMediators of Inflammation0962-93511466-18611993-01-0121737710.1155/S0962935193000109Cytokine and eicosanoid regulation by Schistosoma mansoniduring LSE penetrationA. C. Fusco0B. Salafsky1T. Shibuya2University of Illinois College of Medicine at Rockford, Department of Biomedical Sciences, 1601 Parkview Avenue, Rockford, Illinois 61107, USAUniversity of Illinois College of Medicine at Rockford, Department of Biomedical Sciences, 1601 Parkview Avenue, Rockford, Illinois 61107, USATokyo Medical College, Department of Pharmacology, Tokyo, JapanCercarial penetration, in low to moderate numbers, does not cause a normal skin inflammatory response; therefore, the authors sought to determine whether cercariae can down-regulate keratinocyte activation and thus the secretion of pro-inflammatory cytokines and eicosanoids. Human living skin equivalent (LSE, Organogenesis) consisting of dermal, epidermal and stratum corneum-like layers was used as the skin substrate. The surface of the LSE membrane was exposed to 100 ng IFNγ or ~850 cercariae for 18 h. Incubation media and tissue was then assayed for IL-1α, IL-6, IL-8, TNFα, 5-HETE, 12-HETE, PGF2, LTB4, and LTC4 via RIA and Western Blots. TNFα was not detected. Secreted IL-1α levels were (mean ± S.E.M. (n)): Control, 1.03 ng ± 0.15 (11); IFNγ 1.90 ng ± 0.48 (5); cercariae, 1.79 ng ± 0.22 (22). In spite of this increase, cercariae down-regulated IL-8 (cercariae 11.13 ± 1.70 ng vs. IFNγ = 16.47 ± 0.29 ng, p = 0.04) and LTB4 (cercariae = 98.86 ± 19.65 pg/0.1 ml vs. IFNγ = 193.42 ± 44.21 pg/0.1 ml p = 0.02). No changes were seen in IL-6, 12-HETE, 5-HETE, and PGE2 levels. It is concluded that cercarial penetration causes a release of IL-1α consistent with skin trauma; however, schistosomulae may regulate the production of chemotactic (neutrophils, macrophages, T-cells, etc.) and activation factors such as IL-8 and LTB4.http://dx.doi.org/10.1155/S0962935193000109 |
| spellingShingle | A. C. Fusco B. Salafsky T. Shibuya Cytokine and eicosanoid regulation by Schistosoma mansoniduring LSE penetration Mediators of Inflammation |
| title | Cytokine and eicosanoid regulation by Schistosoma mansoniduring LSE penetration |
| title_full | Cytokine and eicosanoid regulation by Schistosoma mansoniduring LSE penetration |
| title_fullStr | Cytokine and eicosanoid regulation by Schistosoma mansoniduring LSE penetration |
| title_full_unstemmed | Cytokine and eicosanoid regulation by Schistosoma mansoniduring LSE penetration |
| title_short | Cytokine and eicosanoid regulation by Schistosoma mansoniduring LSE penetration |
| title_sort | cytokine and eicosanoid regulation by schistosoma mansoniduring lse penetration |
| url | http://dx.doi.org/10.1155/S0962935193000109 |
| work_keys_str_mv | AT acfusco cytokineandeicosanoidregulationbyschistosomamansoniduringlsepenetration AT bsalafsky cytokineandeicosanoidregulationbyschistosomamansoniduringlsepenetration AT tshibuya cytokineandeicosanoidregulationbyschistosomamansoniduringlsepenetration |