Identification and Utilization of the Soybean Plant Height Locus PH19-31
Plant height is a key agronomic trait in soybeans, directly influencing yield potential and agronomic performance. In this study, the tall-stem cultivar Heinong 63 and the dwarf-stem cultivar Longken 3077, along with their F<sub>2</sub> populations, were used as experimental materials. A...
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MDPI AG
2025-05-01
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| Series: | Agronomy |
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| Online Access: | https://www.mdpi.com/2073-4395/15/6/1316 |
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| author | Manqing Ling Xin Su Xiping Hu Xiang Zhang Yongzhe Gu Zhangxiong Liu Jiankun Xie Lijuan Qiu |
| author_facet | Manqing Ling Xin Su Xiping Hu Xiang Zhang Yongzhe Gu Zhangxiong Liu Jiankun Xie Lijuan Qiu |
| author_sort | Manqing Ling |
| collection | DOAJ |
| description | Plant height is a key agronomic trait in soybeans, directly influencing yield potential and agronomic performance. In this study, the tall-stem cultivar Heinong 63 and the dwarf-stem cultivar Longken 3077, along with their F<sub>2</sub> populations, were used as experimental materials. A preliminary mapping using the bulk segregant analysis sequencing (BSA-Seq) approach enabled us to locate the target locus within a 1.8 Mb interval, which was subsequently narrowed to a 48.3 kb region using simple sequence repeat (SSR) and insertion and deletion (InDel) markers. The interval was designated as PH19-31 and contained nine annotated genes. One InDel marker, PL7, was developed within this fine-mapped region. Based on an expression analysis of the nine candidate genes, <i>Glyma.19G230000</i> and <i>Glyma.19G230200</i> were identified as potential regulators of plant height in soybeans. This study provides valuable materials and technical support for the cloning of plant height-related genes and the molecular breeding of soybeans. |
| format | Article |
| id | doaj-art-b086391a77bf41f59684d71b46e7c3b7 |
| institution | Kabale University |
| issn | 2073-4395 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agronomy |
| spelling | doaj-art-b086391a77bf41f59684d71b46e7c3b72025-08-20T03:30:28ZengMDPI AGAgronomy2073-43952025-05-01156131610.3390/agronomy15061316Identification and Utilization of the Soybean Plant Height Locus PH19-31Manqing Ling0Xin Su1Xiping Hu2Xiang Zhang3Yongzhe Gu4Zhangxiong Liu5Jiankun Xie6Lijuan Qiu7College of Life Sciences, Jiangxi Normal University, Nanchang 330022, ChinaState Key Laboratory of Crop Gene Resources and Breeding, The National Key Facility for Crop Gene Resources and Genetic Improvement (NFCRI), Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaBeidahuang Kenfeng Seed Industry Co., Ltd., Harbin 150090, ChinaState Key Laboratory of Crop Gene Resources and Breeding, The National Key Facility for Crop Gene Resources and Genetic Improvement (NFCRI), Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaState Key Laboratory of Crop Gene Resources and Breeding, The National Key Facility for Crop Gene Resources and Genetic Improvement (NFCRI), Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaState Key Laboratory of Crop Gene Resources and Breeding, The National Key Facility for Crop Gene Resources and Genetic Improvement (NFCRI), Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaCollege of Life Sciences, Jiangxi Normal University, Nanchang 330022, ChinaState Key Laboratory of Crop Gene Resources and Breeding, The National Key Facility for Crop Gene Resources and Genetic Improvement (NFCRI), Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaPlant height is a key agronomic trait in soybeans, directly influencing yield potential and agronomic performance. In this study, the tall-stem cultivar Heinong 63 and the dwarf-stem cultivar Longken 3077, along with their F<sub>2</sub> populations, were used as experimental materials. A preliminary mapping using the bulk segregant analysis sequencing (BSA-Seq) approach enabled us to locate the target locus within a 1.8 Mb interval, which was subsequently narrowed to a 48.3 kb region using simple sequence repeat (SSR) and insertion and deletion (InDel) markers. The interval was designated as PH19-31 and contained nine annotated genes. One InDel marker, PL7, was developed within this fine-mapped region. Based on an expression analysis of the nine candidate genes, <i>Glyma.19G230000</i> and <i>Glyma.19G230200</i> were identified as potential regulators of plant height in soybeans. This study provides valuable materials and technical support for the cloning of plant height-related genes and the molecular breeding of soybeans.https://www.mdpi.com/2073-4395/15/6/1316soybeanBSA-SeqSSR markersInDel markergene mappingmarker-assisted selection |
| spellingShingle | Manqing Ling Xin Su Xiping Hu Xiang Zhang Yongzhe Gu Zhangxiong Liu Jiankun Xie Lijuan Qiu Identification and Utilization of the Soybean Plant Height Locus PH19-31 Agronomy soybean BSA-Seq SSR markers InDel marker gene mapping marker-assisted selection |
| title | Identification and Utilization of the Soybean Plant Height Locus PH19-31 |
| title_full | Identification and Utilization of the Soybean Plant Height Locus PH19-31 |
| title_fullStr | Identification and Utilization of the Soybean Plant Height Locus PH19-31 |
| title_full_unstemmed | Identification and Utilization of the Soybean Plant Height Locus PH19-31 |
| title_short | Identification and Utilization of the Soybean Plant Height Locus PH19-31 |
| title_sort | identification and utilization of the soybean plant height locus ph19 31 |
| topic | soybean BSA-Seq SSR markers InDel marker gene mapping marker-assisted selection |
| url | https://www.mdpi.com/2073-4395/15/6/1316 |
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