Polarity‐selective Transfer of Lipophilic Cargoes From Lipid Droplets (Oleosomes) to Lipid Bilayers
Abstract Lipid Droplets (LDs) or as also called oleosomes are lipid storage organelles in eukaryotic cells. Besides storing lipids, LDs can fuse their core into other intracellular organelles, but the mechanism remains unknown. In this work, this is aimed to understand the effect of cargo's pol...
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| Format: | Article |
| Language: | English |
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Wiley-VCH
2025-03-01
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| Series: | Advanced Materials Interfaces |
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| Online Access: | https://doi.org/10.1002/admi.202400600 |
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| author | Umay Sevgi Vardar Johannes H. Bitter Constantinos V. Nikiforidis |
| author_facet | Umay Sevgi Vardar Johannes H. Bitter Constantinos V. Nikiforidis |
| author_sort | Umay Sevgi Vardar |
| collection | DOAJ |
| description | Abstract Lipid Droplets (LDs) or as also called oleosomes are lipid storage organelles in eukaryotic cells. Besides storing lipids, LDs can fuse their core into other intracellular organelles, but the mechanism remains unknown. In this work, this is aimed to understand the effect of cargo's polarity on the transportation of the cargo from LDs to lipid bilayers using liposomes. LDs are loaded with curcumin and Nile red, two lipophilic molecules with similar log P values. The loaded LDs are blended with liposomes, while curcumin and Nile red are tracked using confocal microscopy and spectroscopy. LDs remained intact, while curcumin was transferred in 5 min from LDs to liposomes. Nile red remained in LDs. The difference between curcumin and Nile red is attributed to the amphiphilicity of curcumin, which allowed its adsorption in the LD monolayer and the subsequent transportation to the liposome bilayer upon contact. The unique selectivity of LDs is shown as carriers since lipophilic cargo is transferred to the lipid bilayer only when participating in the LD membrane. The understanding of the transportation mechanism of molecules from LDs to bilayers helps the exploitation of LDs as natural lipid carriers. |
| format | Article |
| id | doaj-art-b07c56e648304bf4919e805fcb5e4ac5 |
| institution | DOAJ |
| issn | 2196-7350 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Wiley-VCH |
| record_format | Article |
| series | Advanced Materials Interfaces |
| spelling | doaj-art-b07c56e648304bf4919e805fcb5e4ac52025-08-20T03:12:48ZengWiley-VCHAdvanced Materials Interfaces2196-73502025-03-01125n/an/a10.1002/admi.202400600Polarity‐selective Transfer of Lipophilic Cargoes From Lipid Droplets (Oleosomes) to Lipid BilayersUmay Sevgi Vardar0Johannes H. Bitter1Constantinos V. Nikiforidis2Biobased Chemistry and Technology Group Wageningen University Bornse Weilanden 9 Wageningen 6708 WG The NetherlandsBiobased Chemistry and Technology Group Wageningen University Bornse Weilanden 9 Wageningen 6708 WG The NetherlandsBiobased Chemistry and Technology Group Wageningen University Bornse Weilanden 9 Wageningen 6708 WG The NetherlandsAbstract Lipid Droplets (LDs) or as also called oleosomes are lipid storage organelles in eukaryotic cells. Besides storing lipids, LDs can fuse their core into other intracellular organelles, but the mechanism remains unknown. In this work, this is aimed to understand the effect of cargo's polarity on the transportation of the cargo from LDs to lipid bilayers using liposomes. LDs are loaded with curcumin and Nile red, two lipophilic molecules with similar log P values. The loaded LDs are blended with liposomes, while curcumin and Nile red are tracked using confocal microscopy and spectroscopy. LDs remained intact, while curcumin was transferred in 5 min from LDs to liposomes. Nile red remained in LDs. The difference between curcumin and Nile red is attributed to the amphiphilicity of curcumin, which allowed its adsorption in the LD monolayer and the subsequent transportation to the liposome bilayer upon contact. The unique selectivity of LDs is shown as carriers since lipophilic cargo is transferred to the lipid bilayer only when participating in the LD membrane. The understanding of the transportation mechanism of molecules from LDs to bilayers helps the exploitation of LDs as natural lipid carriers.https://doi.org/10.1002/admi.202400600curcuminencapsulationlipid bilayerslipid carriersliposomes |
| spellingShingle | Umay Sevgi Vardar Johannes H. Bitter Constantinos V. Nikiforidis Polarity‐selective Transfer of Lipophilic Cargoes From Lipid Droplets (Oleosomes) to Lipid Bilayers Advanced Materials Interfaces curcumin encapsulation lipid bilayers lipid carriers liposomes |
| title | Polarity‐selective Transfer of Lipophilic Cargoes From Lipid Droplets (Oleosomes) to Lipid Bilayers |
| title_full | Polarity‐selective Transfer of Lipophilic Cargoes From Lipid Droplets (Oleosomes) to Lipid Bilayers |
| title_fullStr | Polarity‐selective Transfer of Lipophilic Cargoes From Lipid Droplets (Oleosomes) to Lipid Bilayers |
| title_full_unstemmed | Polarity‐selective Transfer of Lipophilic Cargoes From Lipid Droplets (Oleosomes) to Lipid Bilayers |
| title_short | Polarity‐selective Transfer of Lipophilic Cargoes From Lipid Droplets (Oleosomes) to Lipid Bilayers |
| title_sort | polarity selective transfer of lipophilic cargoes from lipid droplets oleosomes to lipid bilayers |
| topic | curcumin encapsulation lipid bilayers lipid carriers liposomes |
| url | https://doi.org/10.1002/admi.202400600 |
| work_keys_str_mv | AT umaysevgivardar polarityselectivetransferoflipophiliccargoesfromlipiddropletsoleosomestolipidbilayers AT johanneshbitter polarityselectivetransferoflipophiliccargoesfromlipiddropletsoleosomestolipidbilayers AT constantinosvnikiforidis polarityselectivetransferoflipophiliccargoesfromlipiddropletsoleosomestolipidbilayers |