A New Nanocomposite for Inducing Demineralized Dentin Remineralization

Guifei Ban,1,2,* Jindong Long,1,2,* Kaiqi Yan,1,2,* Qiurong Li,1,2 Xiaoman Huang,1,2 Xiaolang Wei,1,2 Fangfang Xie1,2 1College & Hospital of Stomatology, Guangxi Medical University, Nanning, Guangxi, 530021, People’s Republic of China; 2Guangxi Key Laboratory of Oral and...

Full description

Saved in:
Bibliographic Details
Main Authors: Ban G, Long J, Yan K, Li Q, Huang X, Wei X, Xie F
Format: Article
Language:English
Published: Dove Medical Press 2025-02-01
Series:International Journal of Nanomedicine
Subjects:
Online Access:https://www.dovepress.com/a-new-nanocomposite-for-inducing-demineralized-dentin-remineralization-peer-reviewed-fulltext-article-IJN
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1832570232892293120
author Ban G
Long J
Yan K
Li Q
Huang X
Wei X
Xie F
author_facet Ban G
Long J
Yan K
Li Q
Huang X
Wei X
Xie F
author_sort Ban G
collection DOAJ
description Guifei Ban,1,2,* Jindong Long,1,2,* Kaiqi Yan,1,2,* Qiurong Li,1,2 Xiaoman Huang,1,2 Xiaolang Wei,1,2 Fangfang Xie1,2 1College & Hospital of Stomatology, Guangxi Medical University, Nanning, Guangxi, 530021, People’s Republic of China; 2Guangxi Key Laboratory of Oral and Maxillofacial Rehabilitation and Reconstruction, College of Stomatology, Hospital of Stomatology, Guangxi Medical University, Nanning, Guangxi, China; Guangxi Health Commission Key Laboratory of Prevention and Treatment for Oral Infectious Diseases, College of Stomatology, Hospital of Stomatology, Guangxi Medical University, Nanning, Guangxi, 530021, People’s Republic of China*These authors contributed equally to this workCorrespondence: Fangfang Xie, Email xiedualfang@163.comObjective: This study aimed to investigate a method for promoting the remineralization of demineralized dentin collagen fibers, with the objective of enhancing the bonding strength and durability of dentin resin while reducing the incidence of secondary caries.Methods: A mineralized solution, PAMAM-COOH/ACMP-MDP ethanol solution, was prepared, consisting of the dendritic organic macromolecule PAMAM-COOH, magnesium ions, and methacryloyloxydecyl dihydrogen phosphate (MDP). We examined its storage stability. The solution underwent comprehensive characterization using various techniques, including Fourier Transform Infrared Spectroscopy (FTIR), X-Ray Diffraction (XRD), Transmission Electron Microscopy (TEM), STEM-EDX mapping, and Selected Area Electron Diffraction (SAED). Additionally, remineralization of demineralized dentin was induced by both control groups and the PAMAM-COOH/ACMP-MDP group. The effects were observed using a laser scanning confocal fluorescence microscope (CLSM), scanning electron microscopy (SEM), and TEM.Results: The PAMAM-COOH/ACMP-MDP ethanol solution we prepared maintained stable physicochemical properties after two months of storage. It exhibited good dispersibility, retained an amorphous phase, and maintained a nanometer size with excellent stability. The results from CLSM indicated that, compared to the control group, the PAMAM-COOH/ACMP-MDP ethanol solution could induce partial remineralization of demineralized dentin in deeper dentin tubules. The SEM results indicated that the PAMAM-COOH/ACMP-MDP group exhibited distinct characteristics of collagen fiber remineralization, both on the surface and within the deep dentin tubules. The collagen fibers in this group were thicker and more mineralized. Furthermore, significant remineralization features of collagen fibers were observed in the peritubular dentin of the PAMAM-COOH/ACMP-MDP group as evidenced by TEM.Conclusion: The PAMAM-COOH/ACMP-MDP ethanol solution exhibits thermodynamic stability, uniform dispersion, and the capability to induce remineralization of demineralized dentin collagen fibers. These findings highlight the potential of dendritic macromolecules for the biomimetic mineralization of dentin collagen and underscore the feasibility of utilizing ethanol-based primers in the development of new adhesives and their clinical applications.Keywords: nanocomposite, dentin remineralization, polyamidoamine, collagen
format Article
id doaj-art-b05aa8d53991412690951c35d5ae3340
institution Kabale University
issn 1178-2013
language English
publishDate 2025-02-01
publisher Dove Medical Press
record_format Article
series International Journal of Nanomedicine
spelling doaj-art-b05aa8d53991412690951c35d5ae33402025-02-02T15:59:40ZengDove Medical PressInternational Journal of Nanomedicine1178-20132025-02-01Volume 201335134999795A New Nanocomposite for Inducing Demineralized Dentin RemineralizationBan GLong JYan KLi QHuang XWei XXie FGuifei Ban,1,2,* Jindong Long,1,2,* Kaiqi Yan,1,2,* Qiurong Li,1,2 Xiaoman Huang,1,2 Xiaolang Wei,1,2 Fangfang Xie1,2 1College & Hospital of Stomatology, Guangxi Medical University, Nanning, Guangxi, 530021, People’s Republic of China; 2Guangxi Key Laboratory of Oral and Maxillofacial Rehabilitation and Reconstruction, College of Stomatology, Hospital of Stomatology, Guangxi Medical University, Nanning, Guangxi, China; Guangxi Health Commission Key Laboratory of Prevention and Treatment for Oral Infectious Diseases, College of Stomatology, Hospital of Stomatology, Guangxi Medical University, Nanning, Guangxi, 530021, People’s Republic of China*These authors contributed equally to this workCorrespondence: Fangfang Xie, Email xiedualfang@163.comObjective: This study aimed to investigate a method for promoting the remineralization of demineralized dentin collagen fibers, with the objective of enhancing the bonding strength and durability of dentin resin while reducing the incidence of secondary caries.Methods: A mineralized solution, PAMAM-COOH/ACMP-MDP ethanol solution, was prepared, consisting of the dendritic organic macromolecule PAMAM-COOH, magnesium ions, and methacryloyloxydecyl dihydrogen phosphate (MDP). We examined its storage stability. The solution underwent comprehensive characterization using various techniques, including Fourier Transform Infrared Spectroscopy (FTIR), X-Ray Diffraction (XRD), Transmission Electron Microscopy (TEM), STEM-EDX mapping, and Selected Area Electron Diffraction (SAED). Additionally, remineralization of demineralized dentin was induced by both control groups and the PAMAM-COOH/ACMP-MDP group. The effects were observed using a laser scanning confocal fluorescence microscope (CLSM), scanning electron microscopy (SEM), and TEM.Results: The PAMAM-COOH/ACMP-MDP ethanol solution we prepared maintained stable physicochemical properties after two months of storage. It exhibited good dispersibility, retained an amorphous phase, and maintained a nanometer size with excellent stability. The results from CLSM indicated that, compared to the control group, the PAMAM-COOH/ACMP-MDP ethanol solution could induce partial remineralization of demineralized dentin in deeper dentin tubules. The SEM results indicated that the PAMAM-COOH/ACMP-MDP group exhibited distinct characteristics of collagen fiber remineralization, both on the surface and within the deep dentin tubules. The collagen fibers in this group were thicker and more mineralized. Furthermore, significant remineralization features of collagen fibers were observed in the peritubular dentin of the PAMAM-COOH/ACMP-MDP group as evidenced by TEM.Conclusion: The PAMAM-COOH/ACMP-MDP ethanol solution exhibits thermodynamic stability, uniform dispersion, and the capability to induce remineralization of demineralized dentin collagen fibers. These findings highlight the potential of dendritic macromolecules for the biomimetic mineralization of dentin collagen and underscore the feasibility of utilizing ethanol-based primers in the development of new adhesives and their clinical applications.Keywords: nanocomposite, dentin remineralization, polyamidoamine, collagenhttps://www.dovepress.com/a-new-nanocomposite-for-inducing-demineralized-dentin-remineralization-peer-reviewed-fulltext-article-IJNnanocompositedentin remineralizationpolyamidoaminecollagen
spellingShingle Ban G
Long J
Yan K
Li Q
Huang X
Wei X
Xie F
A New Nanocomposite for Inducing Demineralized Dentin Remineralization
International Journal of Nanomedicine
nanocomposite
dentin remineralization
polyamidoamine
collagen
title A New Nanocomposite for Inducing Demineralized Dentin Remineralization
title_full A New Nanocomposite for Inducing Demineralized Dentin Remineralization
title_fullStr A New Nanocomposite for Inducing Demineralized Dentin Remineralization
title_full_unstemmed A New Nanocomposite for Inducing Demineralized Dentin Remineralization
title_short A New Nanocomposite for Inducing Demineralized Dentin Remineralization
title_sort new nanocomposite for inducing demineralized dentin remineralization
topic nanocomposite
dentin remineralization
polyamidoamine
collagen
url https://www.dovepress.com/a-new-nanocomposite-for-inducing-demineralized-dentin-remineralization-peer-reviewed-fulltext-article-IJN
work_keys_str_mv AT bang anewnanocompositeforinducingdemineralizeddentinremineralization
AT longj anewnanocompositeforinducingdemineralizeddentinremineralization
AT yank anewnanocompositeforinducingdemineralizeddentinremineralization
AT liq anewnanocompositeforinducingdemineralizeddentinremineralization
AT huangx anewnanocompositeforinducingdemineralizeddentinremineralization
AT weix anewnanocompositeforinducingdemineralizeddentinremineralization
AT xief anewnanocompositeforinducingdemineralizeddentinremineralization
AT bang newnanocompositeforinducingdemineralizeddentinremineralization
AT longj newnanocompositeforinducingdemineralizeddentinremineralization
AT yank newnanocompositeforinducingdemineralizeddentinremineralization
AT liq newnanocompositeforinducingdemineralizeddentinremineralization
AT huangx newnanocompositeforinducingdemineralizeddentinremineralization
AT weix newnanocompositeforinducingdemineralizeddentinremineralization
AT xief newnanocompositeforinducingdemineralizeddentinremineralization