Cryptosporidium parvum prevalence in a group of Turkish children
Stool samples from two hundred children with diarrhea and from 50 healthy children were examined, by modified Kinyoun's acid-fast staining (MAF), Giemsa staining and direct (DFA) and indirect immunofluorescence antibody (IFA) methods, in order to determine cryptosporidiosis prevalence un...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Hacettepe University Institute of Child Health
1999-04-01
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| Series: | The Turkish Journal of Pediatrics |
| Online Access: | https://turkjpediatr.org/article/view/3219 |
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| _version_ | 1850033419561467904 |
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| author | Y Akyön S Ergüven S Arikan K Yurdakök A Günalp |
| author_facet | Y Akyön S Ergüven S Arikan K Yurdakök A Günalp |
| author_sort | Y Akyön |
| collection | DOAJ |
| description |
Stool samples from two hundred children with diarrhea and from 50 healthy children were examined, by modified Kinyoun's acid-fast staining (MAF), Giemsa staining and direct (DFA) and indirect immunofluorescence antibody (IFA) methods, in order to determine cryptosporidiosis prevalence under the age of 12 and to detect the most efficient identifying method for use in our country. Cryptosporidium oocysts were detected in seven (3.5%) of the cases. None of the samples from the control subjects was found to be positive for Cryptosporidium. Our results indicate that Cryptosporidial oocysts should be detected in children with diarrhea. Modified Kinyoun staining method is practical and reliable for this purpose. Immunofluorescence staining methods can be applied for conformation of the results, if available.
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| format | Article |
| id | doaj-art-b03dda445ed1487d94c70267de339aaf |
| institution | DOAJ |
| issn | 0041-4301 2791-6421 |
| language | English |
| publishDate | 1999-04-01 |
| publisher | Hacettepe University Institute of Child Health |
| record_format | Article |
| series | The Turkish Journal of Pediatrics |
| spelling | doaj-art-b03dda445ed1487d94c70267de339aaf2025-08-20T02:58:14ZengHacettepe University Institute of Child HealthThe Turkish Journal of Pediatrics0041-43012791-64211999-04-01412Cryptosporidium parvum prevalence in a group of Turkish childrenY Akyön0S ErgüvenS ArikanK YurdakökA GünalpDepartment of Microbiology and Clinical Microbiology, Hacettepe University Faculty of Medicine, Ankara. Stool samples from two hundred children with diarrhea and from 50 healthy children were examined, by modified Kinyoun's acid-fast staining (MAF), Giemsa staining and direct (DFA) and indirect immunofluorescence antibody (IFA) methods, in order to determine cryptosporidiosis prevalence under the age of 12 and to detect the most efficient identifying method for use in our country. Cryptosporidium oocysts were detected in seven (3.5%) of the cases. None of the samples from the control subjects was found to be positive for Cryptosporidium. Our results indicate that Cryptosporidial oocysts should be detected in children with diarrhea. Modified Kinyoun staining method is practical and reliable for this purpose. Immunofluorescence staining methods can be applied for conformation of the results, if available. https://turkjpediatr.org/article/view/3219 |
| spellingShingle | Y Akyön S Ergüven S Arikan K Yurdakök A Günalp Cryptosporidium parvum prevalence in a group of Turkish children The Turkish Journal of Pediatrics |
| title | Cryptosporidium parvum prevalence in a group of Turkish children |
| title_full | Cryptosporidium parvum prevalence in a group of Turkish children |
| title_fullStr | Cryptosporidium parvum prevalence in a group of Turkish children |
| title_full_unstemmed | Cryptosporidium parvum prevalence in a group of Turkish children |
| title_short | Cryptosporidium parvum prevalence in a group of Turkish children |
| title_sort | cryptosporidium parvum prevalence in a group of turkish children |
| url | https://turkjpediatr.org/article/view/3219 |
| work_keys_str_mv | AT yakyon cryptosporidiumparvumprevalenceinagroupofturkishchildren AT serguven cryptosporidiumparvumprevalenceinagroupofturkishchildren AT sarikan cryptosporidiumparvumprevalenceinagroupofturkishchildren AT kyurdakok cryptosporidiumparvumprevalenceinagroupofturkishchildren AT agunalp cryptosporidiumparvumprevalenceinagroupofturkishchildren |