Screening, Genome Sequencing and Tolerance Mechanism Analysis of Bile Acid-resistant Pediococcus pentosaceus 32M07
To further investigate the bile acid metabolism and tolerance mechanisms of probiotics, the bile acid resistance of 165 strains of lactic acid bacteria was evaluated and characterized. The strain 32M07, which demonstrated the highest bile salt hydrolase activity, was subjected to whole-genome sequen...
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The editorial department of Science and Technology of Food Industry
2025-09-01
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| Series: | Shipin gongye ke-ji |
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| Online Access: | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2024100169 |
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| author | Qingping HONG Ziqing DI Daqing SUN Xinrui DU Hongfei LI Yuxi YAO Xianglin GE |
| author_facet | Qingping HONG Ziqing DI Daqing SUN Xinrui DU Hongfei LI Yuxi YAO Xianglin GE |
| author_sort | Qingping HONG |
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| description | To further investigate the bile acid metabolism and tolerance mechanisms of probiotics, the bile acid resistance of 165 strains of lactic acid bacteria was evaluated and characterized. The strain 32M07, which demonstrated the highest bile salt hydrolase activity, was subjected to whole-genome sequencing and an analysis of its bile acid resistance mechanisms. The sequencing results indicated that the genome of strain 32M07 consisted of one chromosome and two plasmids, with a total genomic sequence length of 1882986 base pairs, a G+C% content of 37.2%, and encoded 1828 genes, 55 tRNAs, and 15 rRNAs. The 16S rRNA gene and the core genome phylogenetic tree results were consistent, confirming that strain 32M07 was taxonomically classified as Pediococcus pentosaceus. Based on functional annotations and metabolic relationships of bile acid metabolism and transport-related genes in databases including COG, GO, and KEGG, a bile acid metabolic model for Pediococcus pentosaceus 32M07 was constructed. This model revealed the cellular localization of primary bile acid hydrolysis processes (intracellular), the bile acid, taurine, and proton efflux systems in the cell membrane (n=4), the intracellular metabolic pathways of secondary bile acids and glycine, and the encoding genes of all related proteolytic enzymes. Finally, the assessments of the CARD, ResFinder, VFDB, and PHI databases demonstrated that strain 32M07 exhibited good safety at the genetic level. In summary, this study had identified a bile acid-resistant strain of Pediococcus pentosaceus 32M07, and elucidated its bile acid metabolism and tolerance mechanisms at the genomic level. These results not only contribute to the development and application of probiotic functional foods by providing a high-quality microbial resource, but also offer new theoretical insights and references for the study of probiotic bile acid metabolism and tolerance mechanisms. |
| format | Article |
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| institution | Kabale University |
| issn | 1002-0306 |
| language | zho |
| publishDate | 2025-09-01 |
| publisher | The editorial department of Science and Technology of Food Industry |
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| series | Shipin gongye ke-ji |
| spelling | doaj-art-b03bcd834eaa46a29cd1cc05f4f36ffb2025-08-26T01:30:45ZzhoThe editorial department of Science and Technology of Food IndustryShipin gongye ke-ji1002-03062025-09-01461721322310.13386/j.issn1002-0306.20241001692024100169-17Screening, Genome Sequencing and Tolerance Mechanism Analysis of Bile Acid-resistant Pediococcus pentosaceus 32M07Qingping HONG0Ziqing DI1Daqing SUN2Xinrui DU3Hongfei LI4Yuxi YAO5Xianglin GE6National Coarse Cereals Engineering Research Center, Heilongjiang Bayi Agricultural University, Daqing 163319, ChinaNational Coarse Cereals Engineering Research Center, Heilongjiang Bayi Agricultural University, Daqing 163319, ChinaNational Coarse Cereals Engineering Research Center, Heilongjiang Bayi Agricultural University, Daqing 163319, ChinaNational Coarse Cereals Engineering Research Center, Heilongjiang Bayi Agricultural University, Daqing 163319, ChinaNational Coarse Cereals Engineering Research Center, Heilongjiang Bayi Agricultural University, Daqing 163319, ChinaNational Coarse Cereals Engineering Research Center, Heilongjiang Bayi Agricultural University, Daqing 163319, ChinaNational Coarse Cereals Engineering Research Center, Heilongjiang Bayi Agricultural University, Daqing 163319, ChinaTo further investigate the bile acid metabolism and tolerance mechanisms of probiotics, the bile acid resistance of 165 strains of lactic acid bacteria was evaluated and characterized. The strain 32M07, which demonstrated the highest bile salt hydrolase activity, was subjected to whole-genome sequencing and an analysis of its bile acid resistance mechanisms. The sequencing results indicated that the genome of strain 32M07 consisted of one chromosome and two plasmids, with a total genomic sequence length of 1882986 base pairs, a G+C% content of 37.2%, and encoded 1828 genes, 55 tRNAs, and 15 rRNAs. The 16S rRNA gene and the core genome phylogenetic tree results were consistent, confirming that strain 32M07 was taxonomically classified as Pediococcus pentosaceus. Based on functional annotations and metabolic relationships of bile acid metabolism and transport-related genes in databases including COG, GO, and KEGG, a bile acid metabolic model for Pediococcus pentosaceus 32M07 was constructed. This model revealed the cellular localization of primary bile acid hydrolysis processes (intracellular), the bile acid, taurine, and proton efflux systems in the cell membrane (n=4), the intracellular metabolic pathways of secondary bile acids and glycine, and the encoding genes of all related proteolytic enzymes. Finally, the assessments of the CARD, ResFinder, VFDB, and PHI databases demonstrated that strain 32M07 exhibited good safety at the genetic level. In summary, this study had identified a bile acid-resistant strain of Pediococcus pentosaceus 32M07, and elucidated its bile acid metabolism and tolerance mechanisms at the genomic level. These results not only contribute to the development and application of probiotic functional foods by providing a high-quality microbial resource, but also offer new theoretical insights and references for the study of probiotic bile acid metabolism and tolerance mechanisms.http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2024100169probioticpediococcus pentosaceusbile acid tolerancebile salt hydrolaseefflux system |
| spellingShingle | Qingping HONG Ziqing DI Daqing SUN Xinrui DU Hongfei LI Yuxi YAO Xianglin GE Screening, Genome Sequencing and Tolerance Mechanism Analysis of Bile Acid-resistant Pediococcus pentosaceus 32M07 Shipin gongye ke-ji probiotic pediococcus pentosaceus bile acid tolerance bile salt hydrolase efflux system |
| title | Screening, Genome Sequencing and Tolerance Mechanism Analysis of Bile Acid-resistant Pediococcus pentosaceus 32M07 |
| title_full | Screening, Genome Sequencing and Tolerance Mechanism Analysis of Bile Acid-resistant Pediococcus pentosaceus 32M07 |
| title_fullStr | Screening, Genome Sequencing and Tolerance Mechanism Analysis of Bile Acid-resistant Pediococcus pentosaceus 32M07 |
| title_full_unstemmed | Screening, Genome Sequencing and Tolerance Mechanism Analysis of Bile Acid-resistant Pediococcus pentosaceus 32M07 |
| title_short | Screening, Genome Sequencing and Tolerance Mechanism Analysis of Bile Acid-resistant Pediococcus pentosaceus 32M07 |
| title_sort | screening genome sequencing and tolerance mechanism analysis of bile acid resistant pediococcus pentosaceus 32m07 |
| topic | probiotic pediococcus pentosaceus bile acid tolerance bile salt hydrolase efflux system |
| url | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2024100169 |
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