Pulmonary toxicity of instilled silver nanoparticles: influence of size, coating and rat strain.

Particle size and surface chemistry are potential determinants of silver nanoparticle (AgNP) respiratory toxicity that may also depend on the lung inflammatory state. We compared the effects of intratracheally-administered AgNPs (20 nm and 110 nm; polyvinylpyrrolidone (PVP) and citrate-capped; 0.1 m...

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Main Authors: Joanna Seiffert, Farhana Hussain, Coen Wiegman, Feng Li, Leo Bey, Warren Baker, Alexandra Porter, Mary P Ryan, Yan Chang, Andrew Gow, Junfeng Zhang, Jie Zhu, Terry D Tetley, Kian Fan Chung
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2015-01-01
Series:PLoS ONE
Online Access:https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0119726&type=printable
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author Joanna Seiffert
Farhana Hussain
Coen Wiegman
Feng Li
Leo Bey
Warren Baker
Alexandra Porter
Mary P Ryan
Yan Chang
Andrew Gow
Junfeng Zhang
Jie Zhu
Terry D Tetley
Kian Fan Chung
author_facet Joanna Seiffert
Farhana Hussain
Coen Wiegman
Feng Li
Leo Bey
Warren Baker
Alexandra Porter
Mary P Ryan
Yan Chang
Andrew Gow
Junfeng Zhang
Jie Zhu
Terry D Tetley
Kian Fan Chung
author_sort Joanna Seiffert
collection DOAJ
description Particle size and surface chemistry are potential determinants of silver nanoparticle (AgNP) respiratory toxicity that may also depend on the lung inflammatory state. We compared the effects of intratracheally-administered AgNPs (20 nm and 110 nm; polyvinylpyrrolidone (PVP) and citrate-capped; 0.1 mg/Kg) in Brown-Norway (BN) and Sprague-Dawley (SD) rats. In BN rats, there was both a neutrophilic and eosinophilic response, while in SD rats, there was a neutrophilic response at day 1, greatest for the 20 nm citrate-capped AgNPs. Eosinophilic cationic protein was increased in bronchoalveolar lavage (BAL) in BN and SD rats on day 1. BAL protein and malondialdehyde levels were increased in BN rats at 1 and 7 days, and BAL KC, CCL11 and IL-13 levels at day 1, with increased expression of CCL11 in lung tissue. Pulmonary resistance increased and compliance decreased at day 1, with persistence at day 7. The 20 nm, but not the 110 nm, AgNPs increased bronchial hyperresponsiveness on day 1, which continued at day 7 for the citrate-capped AgNPs only. The 20 nm versus the 110 nm size were more proinflammatory in terms of neutrophil influx, but there was little difference between the citrate-capped versus the PVP-capped AgNPs. AgNPs can induce pulmonary eosinophilic and neutrophilic inflammation with bronchial hyperresponsiveness, features characteristic of asthma.
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spelling doaj-art-b034000321f74cefa4226d206c0a72b12025-08-20T03:10:07ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-01103e011972610.1371/journal.pone.0119726Pulmonary toxicity of instilled silver nanoparticles: influence of size, coating and rat strain.Joanna SeiffertFarhana HussainCoen WiegmanFeng LiLeo BeyWarren BakerAlexandra PorterMary P RyanYan ChangAndrew GowJunfeng ZhangJie ZhuTerry D TetleyKian Fan ChungParticle size and surface chemistry are potential determinants of silver nanoparticle (AgNP) respiratory toxicity that may also depend on the lung inflammatory state. We compared the effects of intratracheally-administered AgNPs (20 nm and 110 nm; polyvinylpyrrolidone (PVP) and citrate-capped; 0.1 mg/Kg) in Brown-Norway (BN) and Sprague-Dawley (SD) rats. In BN rats, there was both a neutrophilic and eosinophilic response, while in SD rats, there was a neutrophilic response at day 1, greatest for the 20 nm citrate-capped AgNPs. Eosinophilic cationic protein was increased in bronchoalveolar lavage (BAL) in BN and SD rats on day 1. BAL protein and malondialdehyde levels were increased in BN rats at 1 and 7 days, and BAL KC, CCL11 and IL-13 levels at day 1, with increased expression of CCL11 in lung tissue. Pulmonary resistance increased and compliance decreased at day 1, with persistence at day 7. The 20 nm, but not the 110 nm, AgNPs increased bronchial hyperresponsiveness on day 1, which continued at day 7 for the citrate-capped AgNPs only. The 20 nm versus the 110 nm size were more proinflammatory in terms of neutrophil influx, but there was little difference between the citrate-capped versus the PVP-capped AgNPs. AgNPs can induce pulmonary eosinophilic and neutrophilic inflammation with bronchial hyperresponsiveness, features characteristic of asthma.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0119726&type=printable
spellingShingle Joanna Seiffert
Farhana Hussain
Coen Wiegman
Feng Li
Leo Bey
Warren Baker
Alexandra Porter
Mary P Ryan
Yan Chang
Andrew Gow
Junfeng Zhang
Jie Zhu
Terry D Tetley
Kian Fan Chung
Pulmonary toxicity of instilled silver nanoparticles: influence of size, coating and rat strain.
PLoS ONE
title Pulmonary toxicity of instilled silver nanoparticles: influence of size, coating and rat strain.
title_full Pulmonary toxicity of instilled silver nanoparticles: influence of size, coating and rat strain.
title_fullStr Pulmonary toxicity of instilled silver nanoparticles: influence of size, coating and rat strain.
title_full_unstemmed Pulmonary toxicity of instilled silver nanoparticles: influence of size, coating and rat strain.
title_short Pulmonary toxicity of instilled silver nanoparticles: influence of size, coating and rat strain.
title_sort pulmonary toxicity of instilled silver nanoparticles influence of size coating and rat strain
url https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0119726&type=printable
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