Cytogenetic Changes in T Lymphocytes in Miners
Objective: to study the impact of chromium levels on cytogenetic impairments in T lymphocytes in miners from the south of Kuzbass.Subjects and methods. Peripheral blood leukocytes were cultured, as described by P. S. Moorhead (1960), by using the cytogenetic assay of the obtained specimens. Analysis...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Federal Research and Clinical Center of Intensive Care Medicine and Rehabilitology, Moscow, Russia
2007-12-01
|
| Series: | Общая реаниматология |
| Subjects: | |
| Online Access: | https://www.reanimatology.com/rmt/article/view/899 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849341082578452480 |
|---|---|
| author | A. V. Makhalin L. Yu. Redkokasha V. V. Moroz Yu. A. Churlyaev N. N. Ilyinskikh M. S. Romanova |
| author_facet | A. V. Makhalin L. Yu. Redkokasha V. V. Moroz Yu. A. Churlyaev N. N. Ilyinskikh M. S. Romanova |
| author_sort | A. V. Makhalin |
| collection | DOAJ |
| description | Objective: to study the impact of chromium levels on cytogenetic impairments in T lymphocytes in miners from the south of Kuzbass.Subjects and methods. Peripheral blood leukocytes were cultured, as described by P. S. Moorhead (1960), by using the cytogenetic assay of the obtained specimens. Analysis of chromosomes considered changes in the number of chromosomes (hyper-, hypo-, or polyploid sets of chromosomes) and their structural derangement. Ninety-three miners from Novokuznetsk and Mezhdurechensk (Kemerovo Region) were examined. According to the length of underground service, the miners were divided into 2 groups: 1) 32 miners with a service length of 1—5 years; 2) 31 with a service length of 10—15 years. A control group included 30 employees from the mine maintenance departments. The mean age of the miners was 38.6±3.4 years. Previous coal chemical analysis in a number of mines revealed significant concentrations of chromium compounds (as high as 298 mg/kg in the clarke of 83 mg/kg). Lymphocytic chromium was quantified by the atomic emission method using inductively bound plasma. The statistically data were processed using a package of Statistica 5.01 computer programs.Results. Cytogenetic study and measurement of peripheral blood chromium were made in miners with a various underground service length. They were found to have elevated peripheral blood T lymphocytes with cytogenetic disorders, which correlated with the increased blood chromium levels.Conclusion. Thus, the miners with an underground service length of 10—15 years were ascertained to have high blood chromium levels, which was accompanied by increases in the count of both cells with chromosomal structural aberrations and those with poly- and hyperploidy. |
| format | Article |
| id | doaj-art-b033586ec2e14d97b2e36c00382147df |
| institution | Kabale University |
| issn | 1813-9779 2411-7110 |
| language | English |
| publishDate | 2007-12-01 |
| publisher | Federal Research and Clinical Center of Intensive Care Medicine and Rehabilitology, Moscow, Russia |
| record_format | Article |
| series | Общая реаниматология |
| spelling | doaj-art-b033586ec2e14d97b2e36c00382147df2025-08-20T03:43:43ZengFederal Research and Clinical Center of Intensive Care Medicine and Rehabilitology, Moscow, RussiaОбщая реаниматология1813-97792411-71102007-12-013613914310.15360/1813-9779-2007-6-139-143899Cytogenetic Changes in T Lymphocytes in MinersA. V. Makhalin0L. Yu. Redkokasha1V. V. Moroz2Yu. A. Churlyaev3N. N. Ilyinskikh4M. S. Romanova5Research Institute of General Reanimatology, Russian Academy of Medical Sciences, MoscowResearch Institute of General Reanimatology, Russian Academy of Medical Sciences, MoscowResearch Institute of General Reanimatology, Russian Academy of Medical Sciences, MoscowResearch Institute of General Reanimatology, Russian Academy of Medical Sciences, MoscowSiberian State Medical University, TomskSiberian State Medical University, TomskObjective: to study the impact of chromium levels on cytogenetic impairments in T lymphocytes in miners from the south of Kuzbass.Subjects and methods. Peripheral blood leukocytes were cultured, as described by P. S. Moorhead (1960), by using the cytogenetic assay of the obtained specimens. Analysis of chromosomes considered changes in the number of chromosomes (hyper-, hypo-, or polyploid sets of chromosomes) and their structural derangement. Ninety-three miners from Novokuznetsk and Mezhdurechensk (Kemerovo Region) were examined. According to the length of underground service, the miners were divided into 2 groups: 1) 32 miners with a service length of 1—5 years; 2) 31 with a service length of 10—15 years. A control group included 30 employees from the mine maintenance departments. The mean age of the miners was 38.6±3.4 years. Previous coal chemical analysis in a number of mines revealed significant concentrations of chromium compounds (as high as 298 mg/kg in the clarke of 83 mg/kg). Lymphocytic chromium was quantified by the atomic emission method using inductively bound plasma. The statistically data were processed using a package of Statistica 5.01 computer programs.Results. Cytogenetic study and measurement of peripheral blood chromium were made in miners with a various underground service length. They were found to have elevated peripheral blood T lymphocytes with cytogenetic disorders, which correlated with the increased blood chromium levels.Conclusion. Thus, the miners with an underground service length of 10—15 years were ascertained to have high blood chromium levels, which was accompanied by increases in the count of both cells with chromosomal structural aberrations and those with poly- and hyperploidy.https://www.reanimatology.com/rmt/article/view/899leukocyteslymphocyteschromosomeschromium and its compoundsminers |
| spellingShingle | A. V. Makhalin L. Yu. Redkokasha V. V. Moroz Yu. A. Churlyaev N. N. Ilyinskikh M. S. Romanova Cytogenetic Changes in T Lymphocytes in Miners Общая реаниматология leukocytes lymphocytes chromosomes chromium and its compounds miners |
| title | Cytogenetic Changes in T Lymphocytes in Miners |
| title_full | Cytogenetic Changes in T Lymphocytes in Miners |
| title_fullStr | Cytogenetic Changes in T Lymphocytes in Miners |
| title_full_unstemmed | Cytogenetic Changes in T Lymphocytes in Miners |
| title_short | Cytogenetic Changes in T Lymphocytes in Miners |
| title_sort | cytogenetic changes in t lymphocytes in miners |
| topic | leukocytes lymphocytes chromosomes chromium and its compounds miners |
| url | https://www.reanimatology.com/rmt/article/view/899 |
| work_keys_str_mv | AT avmakhalin cytogeneticchangesintlymphocytesinminers AT lyuredkokasha cytogeneticchangesintlymphocytesinminers AT vvmoroz cytogeneticchangesintlymphocytesinminers AT yuachurlyaev cytogeneticchangesintlymphocytesinminers AT nnilyinskikh cytogeneticchangesintlymphocytesinminers AT msromanova cytogeneticchangesintlymphocytesinminers |