Clinical phenotypes of Alzheimer’s disease: investigating atrophy patterns and their pathological correlates

Abstract Background In Alzheimer’s disease (AD), MRI atrophy patterns can distinguish between amnestic (typical) and non-amnestic (atypical) clinical phenotypes and are increasingly used for diagnosis and outcome measures in clinical trials. However, understanding how protein accumulation and other...

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Main Authors: Niels Reijner, I. Frigerio, M. M. A. Bouwman, B. D. C. Boon, N. Guizard, T. Jubault, J. J. M. Hoozemans, A. J. M. Rozemuller, F. H. Bouwman, F. Barkhof, E. Gordon, W. D. J. van de Berg, L. E. Jonkman
Format: Article
Language:English
Published: BMC 2025-04-01
Series:Alzheimer’s Research & Therapy
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Online Access:https://doi.org/10.1186/s13195-025-01727-5
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author Niels Reijner
I. Frigerio
M. M. A. Bouwman
B. D. C. Boon
N. Guizard
T. Jubault
J. J. M. Hoozemans
A. J. M. Rozemuller
F. H. Bouwman
F. Barkhof
E. Gordon
W. D. J. van de Berg
L. E. Jonkman
author_facet Niels Reijner
I. Frigerio
M. M. A. Bouwman
B. D. C. Boon
N. Guizard
T. Jubault
J. J. M. Hoozemans
A. J. M. Rozemuller
F. H. Bouwman
F. Barkhof
E. Gordon
W. D. J. van de Berg
L. E. Jonkman
author_sort Niels Reijner
collection DOAJ
description Abstract Background In Alzheimer’s disease (AD), MRI atrophy patterns can distinguish between amnestic (typical) and non-amnestic (atypical) clinical phenotypes and are increasingly used for diagnosis and outcome measures in clinical trials. However, understanding how protein accumulation and other key features of neurodegeneration influence these imaging measurements, are lacking. The current study aimed to assess regional MRI patterns of cortical atrophy across clinical AD phenotypes, and their association with amyloid-beta (Aβ), phosphorylated tau (pTau), neuro-axonal degeneration and microvascular deterioration. Methods Post-mortem in-situ 3DT1 3 T-MRI data was obtained from 33 AD (17 typical, 16 atypical) and 16 control brain donors. Additionally, ante-mortem 3DT1 3 T-MRI scans of brain donors were collected if available. Regional volumes were obtained from MRI scans using an atlas based parcellation software. Eight cortical brain regions were selected from formalin-fixed right hemispheres of brain donors and then immunostained for Aβ, pTau, neurofilament light, and collagen IV. Group comparisons and volume-pathology associations were analyzed using linear mixed models corrected for age, sex, post-mortem delay, and intracranial volume. Results Compared to controls, both typical and atypical AD showed volume loss in the temporo-occipital cortex, while typical AD showed additional volume loss in the parietal cortex. Posterior cingulate volume was lower in typical AD compared to atypical AD (- 6.9%, p = 0.043). In AD, a global positive association between MRI cortical volume and Aβ load (βs = 0.21, p = 0.010), and a global negative association with NfL load (βs = - 0.18, p = 0.018) were observed. Regionally, higher superior parietal gyrus volume was associated with higher Aβ load in typical AD (βs = 0.47, p = 0.004), lower middle frontal gyrus volume associated with higher NfL load in atypical AD (βs = - 0.50, p < 0.001), and lower hippocampal volume associated with higher COLIV load in typical AD (βs = - 1.69, p < 0.001). Comparing post-mortem with ante-mortem scans showed minimal volume differences at scan-intervals within 2 years, highlighting the translational aspect of this study. Conclusion For both clinical phenotypes, cortical volume is affected by Aβ and neuro-axonal damage, but in opposing directions. Differences in volume-pathology relationships between clinical phenotypes are region-specific. The findings of this study could improve the interpretation of MRI datasets in heterogenous AD cohorts, both in research and clinical settings.
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spelling doaj-art-b030e490f34a4ee8968d020ccdfd01e52025-08-20T02:20:06ZengBMCAlzheimer’s Research & Therapy1758-91932025-04-0117111810.1186/s13195-025-01727-5Clinical phenotypes of Alzheimer’s disease: investigating atrophy patterns and their pathological correlatesNiels Reijner0I. Frigerio1M. M. A. Bouwman2B. D. C. Boon3N. Guizard4T. Jubault5J. J. M. Hoozemans6A. J. M. Rozemuller7F. H. Bouwman8F. Barkhof9E. Gordon10W. D. J. van de Berg11L. E. Jonkman12Department of Anatomy and Neurosciences, Amsterdam UMC, Vrije Universiteit AmsterdamDepartment of Anatomy and Neurosciences, Amsterdam UMC, Vrije Universiteit AmsterdamDepartment of Anatomy and Neurosciences, Amsterdam UMC, Vrije Universiteit AmsterdamDepartment of Neuroscience, Mayo ClinicQynapseQynapsePrograms of Neurodegeneration, Amsterdam NeurosciencePrograms of Neurodegeneration, Amsterdam NeuroscienceDepartment of Neurology, Amsterdam UMC, Vrije Universiteit AmsterdamDepartment of Neurology, Amsterdam UMC, Vrije Universiteit AmsterdamQynapseDepartment of Anatomy and Neurosciences, Amsterdam UMC, Vrije Universiteit AmsterdamDepartment of Anatomy and Neurosciences, Amsterdam UMC, Vrije Universiteit AmsterdamAbstract Background In Alzheimer’s disease (AD), MRI atrophy patterns can distinguish between amnestic (typical) and non-amnestic (atypical) clinical phenotypes and are increasingly used for diagnosis and outcome measures in clinical trials. However, understanding how protein accumulation and other key features of neurodegeneration influence these imaging measurements, are lacking. The current study aimed to assess regional MRI patterns of cortical atrophy across clinical AD phenotypes, and their association with amyloid-beta (Aβ), phosphorylated tau (pTau), neuro-axonal degeneration and microvascular deterioration. Methods Post-mortem in-situ 3DT1 3 T-MRI data was obtained from 33 AD (17 typical, 16 atypical) and 16 control brain donors. Additionally, ante-mortem 3DT1 3 T-MRI scans of brain donors were collected if available. Regional volumes were obtained from MRI scans using an atlas based parcellation software. Eight cortical brain regions were selected from formalin-fixed right hemispheres of brain donors and then immunostained for Aβ, pTau, neurofilament light, and collagen IV. Group comparisons and volume-pathology associations were analyzed using linear mixed models corrected for age, sex, post-mortem delay, and intracranial volume. Results Compared to controls, both typical and atypical AD showed volume loss in the temporo-occipital cortex, while typical AD showed additional volume loss in the parietal cortex. Posterior cingulate volume was lower in typical AD compared to atypical AD (- 6.9%, p = 0.043). In AD, a global positive association between MRI cortical volume and Aβ load (βs = 0.21, p = 0.010), and a global negative association with NfL load (βs = - 0.18, p = 0.018) were observed. Regionally, higher superior parietal gyrus volume was associated with higher Aβ load in typical AD (βs = 0.47, p = 0.004), lower middle frontal gyrus volume associated with higher NfL load in atypical AD (βs = - 0.50, p < 0.001), and lower hippocampal volume associated with higher COLIV load in typical AD (βs = - 1.69, p < 0.001). Comparing post-mortem with ante-mortem scans showed minimal volume differences at scan-intervals within 2 years, highlighting the translational aspect of this study. Conclusion For both clinical phenotypes, cortical volume is affected by Aβ and neuro-axonal damage, but in opposing directions. Differences in volume-pathology relationships between clinical phenotypes are region-specific. The findings of this study could improve the interpretation of MRI datasets in heterogenous AD cohorts, both in research and clinical settings.https://doi.org/10.1186/s13195-025-01727-5Alzheimer’s diseaseCortical volumeClinical phenotypesPathological featuresPost-mortem
spellingShingle Niels Reijner
I. Frigerio
M. M. A. Bouwman
B. D. C. Boon
N. Guizard
T. Jubault
J. J. M. Hoozemans
A. J. M. Rozemuller
F. H. Bouwman
F. Barkhof
E. Gordon
W. D. J. van de Berg
L. E. Jonkman
Clinical phenotypes of Alzheimer’s disease: investigating atrophy patterns and their pathological correlates
Alzheimer’s Research & Therapy
Alzheimer’s disease
Cortical volume
Clinical phenotypes
Pathological features
Post-mortem
title Clinical phenotypes of Alzheimer’s disease: investigating atrophy patterns and their pathological correlates
title_full Clinical phenotypes of Alzheimer’s disease: investigating atrophy patterns and their pathological correlates
title_fullStr Clinical phenotypes of Alzheimer’s disease: investigating atrophy patterns and their pathological correlates
title_full_unstemmed Clinical phenotypes of Alzheimer’s disease: investigating atrophy patterns and their pathological correlates
title_short Clinical phenotypes of Alzheimer’s disease: investigating atrophy patterns and their pathological correlates
title_sort clinical phenotypes of alzheimer s disease investigating atrophy patterns and their pathological correlates
topic Alzheimer’s disease
Cortical volume
Clinical phenotypes
Pathological features
Post-mortem
url https://doi.org/10.1186/s13195-025-01727-5
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