Helium and argon cold plasma effects on the 4T1 cancer cells and a triple negative mouse model of breast cancer
Abstract Nowadays, cold atmospheric plasma (CAP) technology has developed as an innovative tool for cancer therapy. Although many studies have reported the antitumor effects of plasma in vivo and in vitro, there are many challenges, including standardization of plasma devices and treatment time for...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2025-03-01
|
| Series: | Scientific Reports |
| Subjects: | |
| Online Access: | https://doi.org/10.1038/s41598-025-95065-z |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849391036371042304 |
|---|---|
| author | Mahdiyeh Bakhtiyari-Ramezani Meysam Nasiri Mansoureh Baniasadi |
| author_facet | Mahdiyeh Bakhtiyari-Ramezani Meysam Nasiri Mansoureh Baniasadi |
| author_sort | Mahdiyeh Bakhtiyari-Ramezani |
| collection | DOAJ |
| description | Abstract Nowadays, cold atmospheric plasma (CAP) technology has developed as an innovative tool for cancer therapy. Although many studies have reported the antitumor effects of plasma in vivo and in vitro, there are many challenges, including standardization of plasma devices and treatment time for different tumors. For the first time, we aimed to evaluate and compare optimal exposure time and direction-dependent cellular effects of helium and argon plasma on the 4T1 cancer cells and a triple-negative mouse model of breast cancer. This study used two types of helium and argon plasma jet devices with different input parameters. In vitro evaluations on 4T1 cell line using the MTT assays and flow cytometry analysis demonstrate CAP-induced apoptosis in all treated groups, especially in the direct approach. These changes were concurrent with increased intracellular reactive oxygen species levels and decreased total antioxidant capacity in these cells. In vivo studies concurrent with in vitro results revealed that CAP therapy reduces tumor size, decreases Nottingham histological score, prevents weight loss, and increases the survival rate in all treated groups. These results suggest that plasma therapy may overcome the adverse effects of approved cancer therapeutic strategies and seems to be a significant issue for cancer patients in the clinical stage, alone or in combination with current therapeutic programs. |
| format | Article |
| id | doaj-art-b02fad9900934c978d576957a9be0322 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-b02fad9900934c978d576957a9be03222025-08-20T03:41:12ZengNature PortfolioScientific Reports2045-23222025-03-0115111610.1038/s41598-025-95065-zHelium and argon cold plasma effects on the 4T1 cancer cells and a triple negative mouse model of breast cancerMahdiyeh Bakhtiyari-Ramezani0Meysam Nasiri1Mansoureh Baniasadi2Plasma Physics and Nuclear Fusion Research School, Nuclear Science and Technology Research Institute (NSTRI)Department of Cellular and Molecular Biology, School of Biology, Damghan UniversityDepartment of Biotechnology, Knowledge-Intensive Plasma Technology Development CompanyAbstract Nowadays, cold atmospheric plasma (CAP) technology has developed as an innovative tool for cancer therapy. Although many studies have reported the antitumor effects of plasma in vivo and in vitro, there are many challenges, including standardization of plasma devices and treatment time for different tumors. For the first time, we aimed to evaluate and compare optimal exposure time and direction-dependent cellular effects of helium and argon plasma on the 4T1 cancer cells and a triple-negative mouse model of breast cancer. This study used two types of helium and argon plasma jet devices with different input parameters. In vitro evaluations on 4T1 cell line using the MTT assays and flow cytometry analysis demonstrate CAP-induced apoptosis in all treated groups, especially in the direct approach. These changes were concurrent with increased intracellular reactive oxygen species levels and decreased total antioxidant capacity in these cells. In vivo studies concurrent with in vitro results revealed that CAP therapy reduces tumor size, decreases Nottingham histological score, prevents weight loss, and increases the survival rate in all treated groups. These results suggest that plasma therapy may overcome the adverse effects of approved cancer therapeutic strategies and seems to be a significant issue for cancer patients in the clinical stage, alone or in combination with current therapeutic programs.https://doi.org/10.1038/s41598-025-95065-zNon-thermal plasmaCancer therapyBreast tumor |
| spellingShingle | Mahdiyeh Bakhtiyari-Ramezani Meysam Nasiri Mansoureh Baniasadi Helium and argon cold plasma effects on the 4T1 cancer cells and a triple negative mouse model of breast cancer Scientific Reports Non-thermal plasma Cancer therapy Breast tumor |
| title | Helium and argon cold plasma effects on the 4T1 cancer cells and a triple negative mouse model of breast cancer |
| title_full | Helium and argon cold plasma effects on the 4T1 cancer cells and a triple negative mouse model of breast cancer |
| title_fullStr | Helium and argon cold plasma effects on the 4T1 cancer cells and a triple negative mouse model of breast cancer |
| title_full_unstemmed | Helium and argon cold plasma effects on the 4T1 cancer cells and a triple negative mouse model of breast cancer |
| title_short | Helium and argon cold plasma effects on the 4T1 cancer cells and a triple negative mouse model of breast cancer |
| title_sort | helium and argon cold plasma effects on the 4t1 cancer cells and a triple negative mouse model of breast cancer |
| topic | Non-thermal plasma Cancer therapy Breast tumor |
| url | https://doi.org/10.1038/s41598-025-95065-z |
| work_keys_str_mv | AT mahdiyehbakhtiyariramezani heliumandargoncoldplasmaeffectsonthe4t1cancercellsandatriplenegativemousemodelofbreastcancer AT meysamnasiri heliumandargoncoldplasmaeffectsonthe4t1cancercellsandatriplenegativemousemodelofbreastcancer AT mansourehbaniasadi heliumandargoncoldplasmaeffectsonthe4t1cancercellsandatriplenegativemousemodelofbreastcancer |