Cellular and Molecular Evidence of Acetaldehyde Elimination and Intracellular Environment Antioxidation by L-Cysteine
Acetaldehyde is a harmful metabolite of smoking and drinking. This study was initially intended to facilitate the understanding of the possible injury mechanism of A549 cells damaged by acetaldehyde and the possible protective mechanism of L-cysteine (L-Cys) by analyzing the oxidative damage indicat...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2018-01-01
|
| Series: | Journal of Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2018/6864574 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850176201943941120 |
|---|---|
| author | Zhenjing Li Xiaohong Zhang Yibin Xue Jingkai Zhang Meiling Li Cheng Luo |
| author_facet | Zhenjing Li Xiaohong Zhang Yibin Xue Jingkai Zhang Meiling Li Cheng Luo |
| author_sort | Zhenjing Li |
| collection | DOAJ |
| description | Acetaldehyde is a harmful metabolite of smoking and drinking. This study was initially intended to facilitate the understanding of the possible injury mechanism of A549 cells damaged by acetaldehyde and the possible protective mechanism of L-cysteine (L-Cys) by analyzing the oxidative damage indicators, as well as the changes in cell morphology and gene expression. Results from the dithiodimorpholine nitrobenzoic acid colorimetric determination for glutathione peroxidase (GSH-Px) activity in L-Cys groups were significantly higher (P<0.01) than those in the acetaldehyde group in a dose-dependent manner. The expression of cytochrome c oxidase subunit II (COII) mRNA was significantly reduced compared with the control group (P<0.01) and was noticeably restored in the L-Cys groups. Scanning electronic microscopy observation, DAPI staining, and flow cytometry also indicated that L-Cys could effectively attenuate the oxidative damage to A549 cells caused by acetaldehyde and reduces the rate of apoptosis. In conclusion, the protective effects of L-Cys on A549 cells against oxidative damage by acetaldehyde were dose-dependent within the range of 10 μmol/L to 160 μmol/L. Acetaldehyde damaged the mitochondria and resulted in the apoptosis of A549 cells by reactive oxygen species (ROS), e.g., free radicals, but L-Cys reversed the release of cytochrome c from the mitochondria, reduced the rate of apoptosis, and protected cells from ROS and oxidative stress. |
| format | Article |
| id | doaj-art-b027332c23d741b69d528e13a5584618 |
| institution | OA Journals |
| issn | 2090-9063 2090-9071 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Chemistry |
| spelling | doaj-art-b027332c23d741b69d528e13a55846182025-08-20T02:19:18ZengWileyJournal of Chemistry2090-90632090-90712018-01-01201810.1155/2018/68645746864574Cellular and Molecular Evidence of Acetaldehyde Elimination and Intracellular Environment Antioxidation by L-CysteineZhenjing Li0Xiaohong Zhang1Yibin Xue2Jingkai Zhang3Meiling Li4Cheng Luo5State Key Laboratory of Food Nutrition and Safety, Tianjin University of Science & Technology, Tianjin 300457, ChinaState Key Laboratory of Food Nutrition and Safety, Tianjin University of Science & Technology, Tianjin 300457, ChinaState Key Laboratory of Food Nutrition and Safety, Tianjin University of Science & Technology, Tianjin 300457, ChinaState Key Laboratory of Food Nutrition and Safety, Tianjin University of Science & Technology, Tianjin 300457, ChinaState Key Laboratory of Food Nutrition and Safety, Tianjin University of Science & Technology, Tianjin 300457, ChinaState Key Laboratory of Food Nutrition and Safety, Tianjin University of Science & Technology, Tianjin 300457, ChinaAcetaldehyde is a harmful metabolite of smoking and drinking. This study was initially intended to facilitate the understanding of the possible injury mechanism of A549 cells damaged by acetaldehyde and the possible protective mechanism of L-cysteine (L-Cys) by analyzing the oxidative damage indicators, as well as the changes in cell morphology and gene expression. Results from the dithiodimorpholine nitrobenzoic acid colorimetric determination for glutathione peroxidase (GSH-Px) activity in L-Cys groups were significantly higher (P<0.01) than those in the acetaldehyde group in a dose-dependent manner. The expression of cytochrome c oxidase subunit II (COII) mRNA was significantly reduced compared with the control group (P<0.01) and was noticeably restored in the L-Cys groups. Scanning electronic microscopy observation, DAPI staining, and flow cytometry also indicated that L-Cys could effectively attenuate the oxidative damage to A549 cells caused by acetaldehyde and reduces the rate of apoptosis. In conclusion, the protective effects of L-Cys on A549 cells against oxidative damage by acetaldehyde were dose-dependent within the range of 10 μmol/L to 160 μmol/L. Acetaldehyde damaged the mitochondria and resulted in the apoptosis of A549 cells by reactive oxygen species (ROS), e.g., free radicals, but L-Cys reversed the release of cytochrome c from the mitochondria, reduced the rate of apoptosis, and protected cells from ROS and oxidative stress.http://dx.doi.org/10.1155/2018/6864574 |
| spellingShingle | Zhenjing Li Xiaohong Zhang Yibin Xue Jingkai Zhang Meiling Li Cheng Luo Cellular and Molecular Evidence of Acetaldehyde Elimination and Intracellular Environment Antioxidation by L-Cysteine Journal of Chemistry |
| title | Cellular and Molecular Evidence of Acetaldehyde Elimination and Intracellular Environment Antioxidation by L-Cysteine |
| title_full | Cellular and Molecular Evidence of Acetaldehyde Elimination and Intracellular Environment Antioxidation by L-Cysteine |
| title_fullStr | Cellular and Molecular Evidence of Acetaldehyde Elimination and Intracellular Environment Antioxidation by L-Cysteine |
| title_full_unstemmed | Cellular and Molecular Evidence of Acetaldehyde Elimination and Intracellular Environment Antioxidation by L-Cysteine |
| title_short | Cellular and Molecular Evidence of Acetaldehyde Elimination and Intracellular Environment Antioxidation by L-Cysteine |
| title_sort | cellular and molecular evidence of acetaldehyde elimination and intracellular environment antioxidation by l cysteine |
| url | http://dx.doi.org/10.1155/2018/6864574 |
| work_keys_str_mv | AT zhenjingli cellularandmolecularevidenceofacetaldehydeeliminationandintracellularenvironmentantioxidationbylcysteine AT xiaohongzhang cellularandmolecularevidenceofacetaldehydeeliminationandintracellularenvironmentantioxidationbylcysteine AT yibinxue cellularandmolecularevidenceofacetaldehydeeliminationandintracellularenvironmentantioxidationbylcysteine AT jingkaizhang cellularandmolecularevidenceofacetaldehydeeliminationandintracellularenvironmentantioxidationbylcysteine AT meilingli cellularandmolecularevidenceofacetaldehydeeliminationandintracellularenvironmentantioxidationbylcysteine AT chengluo cellularandmolecularevidenceofacetaldehydeeliminationandintracellularenvironmentantioxidationbylcysteine |