Validation of a HPLC/FLD Method for Quantification of Tocotrienols in Human Plasma
Quantification of tocotrienols in human plasma is critical when the attention towards tocotrienols on its distinctive properties is arising. We aim to develop a simple and practical normal-phase high performance liquid chromatography method to quantify the amount of four tocotrienol homologues in hu...
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | International Journal of Analytical Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2015/357609 |
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| author | Hui-Ling Che Doryn Meam-Yee Tan Puvaneswari Meganathan Yee-Lin Gan Ghazali Abdul Razak Ju-Yen Fu |
| author_facet | Hui-Ling Che Doryn Meam-Yee Tan Puvaneswari Meganathan Yee-Lin Gan Ghazali Abdul Razak Ju-Yen Fu |
| author_sort | Hui-Ling Che |
| collection | DOAJ |
| description | Quantification of tocotrienols in human plasma is critical when the attention towards tocotrienols on its distinctive properties is arising. We aim to develop a simple and practical normal-phase high performance liquid chromatography method to quantify the amount of four tocotrienol homologues in human plasma. Using both the external and internal standards, tocotrienol homologues were quantified via a normal-phase high performance liquid chromatography with fluorescence detector maintained at the excitation wavelength of 295 nm and the emission wavelength of 325 nm. The four tocotrienol homologues were well separated within 30 minutes. A large interindividual variation between subjects was observed as the absorption of tocotrienols is dependent on food matrix and gut lipolysis. The accuracies of lower and upper limit of quantification ranged between 92% and 109% for intraday assays and 90% and 112% for interday assays. This method was successfully applied to quantify the total amount of four tocotrienol homologues in human plasma. |
| format | Article |
| id | doaj-art-b020623857cc4bf09ef4866f03705d83 |
| institution | Kabale University |
| issn | 1687-8760 1687-8779 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Analytical Chemistry |
| spelling | doaj-art-b020623857cc4bf09ef4866f03705d832025-02-03T05:57:45ZengWileyInternational Journal of Analytical Chemistry1687-87601687-87792015-01-01201510.1155/2015/357609357609Validation of a HPLC/FLD Method for Quantification of Tocotrienols in Human PlasmaHui-Ling Che0Doryn Meam-Yee Tan1Puvaneswari Meganathan2Yee-Lin Gan3Ghazali Abdul Razak4Ju-Yen Fu5Malaysian Palm Oil Board (MPOB), No. 6, Persiaran Institusi, Bandar Baru Bangi, 43000 Kajang, Selangor Darul Ehsan, MalaysiaMalaysian Palm Oil Board (MPOB), No. 6, Persiaran Institusi, Bandar Baru Bangi, 43000 Kajang, Selangor Darul Ehsan, MalaysiaMalaysian Palm Oil Board (MPOB), No. 6, Persiaran Institusi, Bandar Baru Bangi, 43000 Kajang, Selangor Darul Ehsan, MalaysiaMalaysian Palm Oil Board (MPOB), No. 6, Persiaran Institusi, Bandar Baru Bangi, 43000 Kajang, Selangor Darul Ehsan, MalaysiaMalaysian Palm Oil Board (MPOB), No. 6, Persiaran Institusi, Bandar Baru Bangi, 43000 Kajang, Selangor Darul Ehsan, MalaysiaMalaysian Palm Oil Board (MPOB), No. 6, Persiaran Institusi, Bandar Baru Bangi, 43000 Kajang, Selangor Darul Ehsan, MalaysiaQuantification of tocotrienols in human plasma is critical when the attention towards tocotrienols on its distinctive properties is arising. We aim to develop a simple and practical normal-phase high performance liquid chromatography method to quantify the amount of four tocotrienol homologues in human plasma. Using both the external and internal standards, tocotrienol homologues were quantified via a normal-phase high performance liquid chromatography with fluorescence detector maintained at the excitation wavelength of 295 nm and the emission wavelength of 325 nm. The four tocotrienol homologues were well separated within 30 minutes. A large interindividual variation between subjects was observed as the absorption of tocotrienols is dependent on food matrix and gut lipolysis. The accuracies of lower and upper limit of quantification ranged between 92% and 109% for intraday assays and 90% and 112% for interday assays. This method was successfully applied to quantify the total amount of four tocotrienol homologues in human plasma.http://dx.doi.org/10.1155/2015/357609 |
| spellingShingle | Hui-Ling Che Doryn Meam-Yee Tan Puvaneswari Meganathan Yee-Lin Gan Ghazali Abdul Razak Ju-Yen Fu Validation of a HPLC/FLD Method for Quantification of Tocotrienols in Human Plasma International Journal of Analytical Chemistry |
| title | Validation of a HPLC/FLD Method for Quantification of Tocotrienols in Human Plasma |
| title_full | Validation of a HPLC/FLD Method for Quantification of Tocotrienols in Human Plasma |
| title_fullStr | Validation of a HPLC/FLD Method for Quantification of Tocotrienols in Human Plasma |
| title_full_unstemmed | Validation of a HPLC/FLD Method for Quantification of Tocotrienols in Human Plasma |
| title_short | Validation of a HPLC/FLD Method for Quantification of Tocotrienols in Human Plasma |
| title_sort | validation of a hplc fld method for quantification of tocotrienols in human plasma |
| url | http://dx.doi.org/10.1155/2015/357609 |
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