BcBRC1a is a negative regulator for tillering in non-heading Chinese cabbage
Shoot branching is a decisive factor for crop yield. Molecular mechanism for regulating shoot branching (tillering) needs to be determined. Plenty of previous studies have illustrated that BRANCHED1 (BRC1) is a key integrator of shoot branching regulating signals. However, BcBRC1 function in non-hea...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Maximum Academic Press
2022-01-01
|
| Series: | Vegetable Research |
| Subjects: | |
| Online Access: | https://www.maxapress.com/article/doi/10.48130/VR-2022-0011 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850200491428937728 |
|---|---|
| author | Yan Long Tianzi Zhao Lanlan Xu Wei Zhang Feiyi Huang Jianjun Wang Xilin Hou Ying Li |
| author_facet | Yan Long Tianzi Zhao Lanlan Xu Wei Zhang Feiyi Huang Jianjun Wang Xilin Hou Ying Li |
| author_sort | Yan Long |
| collection | DOAJ |
| description | Shoot branching is a decisive factor for crop yield. Molecular mechanism for regulating shoot branching (tillering) needs to be determined. Plenty of previous studies have illustrated that BRANCHED1 (BRC1) is a key integrator of shoot branching regulating signals. However, BcBRC1 function in non-heading Chinese cabbage (Brassica campestris ssp. chinensis) (NHCC) remains unknown. Here, we defined two BRC1 orthologs, BcBRC1a and BcBRC1b, from NHCC and focused on the BcBRC1a gene to describe its alternative splicing characteristic and structure. BcBRC1a was expressed rhythmically and mainly in leaf axils at the 'Maertou' cultivar tillering stage. BcBRC1aL encoded a nuclear location protein. Its ectopic expression caused Arabidopsis growth inhibition and silencing BcBRC1a led to increased tiller numbers in 'Maertou'. Removing the shoot tips of NHCC caused axillary buds to be released from apical dominance and BcBRC1a expression down-regulation. Our research determined that BcBRC1a acts as a negative regulator for tillering in non-heading Chinese cabbage and sets the foundation for further studies. |
| format | Article |
| id | doaj-art-b01b2d56f4d9449da3cf22b2b10f77ef |
| institution | OA Journals |
| issn | 2769-0520 |
| language | English |
| publishDate | 2022-01-01 |
| publisher | Maximum Academic Press |
| record_format | Article |
| series | Vegetable Research |
| spelling | doaj-art-b01b2d56f4d9449da3cf22b2b10f77ef2025-08-20T02:12:19ZengMaximum Academic PressVegetable Research2769-05202022-01-01211810.48130/VR-2022-0011VR-2022-0011BcBRC1a is a negative regulator for tillering in non-heading Chinese cabbageYan Long0Tianzi Zhao1Lanlan Xu2Wei Zhang3Feiyi Huang4Jianjun Wang5Xilin Hou6Ying Li7State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, ChinaState Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, ChinaState Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, ChinaState Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, ChinaState Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, ChinaState Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, ChinaState Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, ChinaState Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, ChinaShoot branching is a decisive factor for crop yield. Molecular mechanism for regulating shoot branching (tillering) needs to be determined. Plenty of previous studies have illustrated that BRANCHED1 (BRC1) is a key integrator of shoot branching regulating signals. However, BcBRC1 function in non-heading Chinese cabbage (Brassica campestris ssp. chinensis) (NHCC) remains unknown. Here, we defined two BRC1 orthologs, BcBRC1a and BcBRC1b, from NHCC and focused on the BcBRC1a gene to describe its alternative splicing characteristic and structure. BcBRC1a was expressed rhythmically and mainly in leaf axils at the 'Maertou' cultivar tillering stage. BcBRC1aL encoded a nuclear location protein. Its ectopic expression caused Arabidopsis growth inhibition and silencing BcBRC1a led to increased tiller numbers in 'Maertou'. Removing the shoot tips of NHCC caused axillary buds to be released from apical dominance and BcBRC1a expression down-regulation. Our research determined that BcBRC1a acts as a negative regulator for tillering in non-heading Chinese cabbage and sets the foundation for further studies.https://www.maxapress.com/article/doi/10.48130/VR-2022-0011bcbrc1atilleringnon-heading chinese cabbagealternative splicingdecapitation |
| spellingShingle | Yan Long Tianzi Zhao Lanlan Xu Wei Zhang Feiyi Huang Jianjun Wang Xilin Hou Ying Li BcBRC1a is a negative regulator for tillering in non-heading Chinese cabbage Vegetable Research bcbrc1a tillering non-heading chinese cabbage alternative splicing decapitation |
| title | BcBRC1a is a negative regulator for tillering in non-heading Chinese cabbage |
| title_full | BcBRC1a is a negative regulator for tillering in non-heading Chinese cabbage |
| title_fullStr | BcBRC1a is a negative regulator for tillering in non-heading Chinese cabbage |
| title_full_unstemmed | BcBRC1a is a negative regulator for tillering in non-heading Chinese cabbage |
| title_short | BcBRC1a is a negative regulator for tillering in non-heading Chinese cabbage |
| title_sort | bcbrc1a is a negative regulator for tillering in non heading chinese cabbage |
| topic | bcbrc1a tillering non-heading chinese cabbage alternative splicing decapitation |
| url | https://www.maxapress.com/article/doi/10.48130/VR-2022-0011 |
| work_keys_str_mv | AT yanlong bcbrc1aisanegativeregulatorfortilleringinnonheadingchinesecabbage AT tianzizhao bcbrc1aisanegativeregulatorfortilleringinnonheadingchinesecabbage AT lanlanxu bcbrc1aisanegativeregulatorfortilleringinnonheadingchinesecabbage AT weizhang bcbrc1aisanegativeregulatorfortilleringinnonheadingchinesecabbage AT feiyihuang bcbrc1aisanegativeregulatorfortilleringinnonheadingchinesecabbage AT jianjunwang bcbrc1aisanegativeregulatorfortilleringinnonheadingchinesecabbage AT xilinhou bcbrc1aisanegativeregulatorfortilleringinnonheadingchinesecabbage AT yingli bcbrc1aisanegativeregulatorfortilleringinnonheadingchinesecabbage |