BcBRC1a is a negative regulator for tillering in non-heading Chinese cabbage

Shoot branching is a decisive factor for crop yield. Molecular mechanism for regulating shoot branching (tillering) needs to be determined. Plenty of previous studies have illustrated that BRANCHED1 (BRC1) is a key integrator of shoot branching regulating signals. However, BcBRC1 function in non-hea...

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Bibliographic Details
Main Authors: Yan Long, Tianzi Zhao, Lanlan Xu, Wei Zhang, Feiyi Huang, Jianjun Wang, Xilin Hou, Ying Li
Format: Article
Language:English
Published: Maximum Academic Press 2022-01-01
Series:Vegetable Research
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Online Access:https://www.maxapress.com/article/doi/10.48130/VR-2022-0011
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Summary:Shoot branching is a decisive factor for crop yield. Molecular mechanism for regulating shoot branching (tillering) needs to be determined. Plenty of previous studies have illustrated that BRANCHED1 (BRC1) is a key integrator of shoot branching regulating signals. However, BcBRC1 function in non-heading Chinese cabbage (Brassica campestris ssp. chinensis) (NHCC) remains unknown. Here, we defined two BRC1 orthologs, BcBRC1a and BcBRC1b, from NHCC and focused on the BcBRC1a gene to describe its alternative splicing characteristic and structure. BcBRC1a was expressed rhythmically and mainly in leaf axils at the 'Maertou' cultivar tillering stage. BcBRC1aL encoded a nuclear location protein. Its ectopic expression caused Arabidopsis growth inhibition and silencing BcBRC1a led to increased tiller numbers in 'Maertou'. Removing the shoot tips of NHCC caused axillary buds to be released from apical dominance and BcBRC1a expression down-regulation. Our research determined that BcBRC1a acts as a negative regulator for tillering in non-heading Chinese cabbage and sets the foundation for further studies.
ISSN:2769-0520