The Oxidative State of Chylomicron Remnants Influences Their Modulation of Human Monocyte Activation
Chylomicron remnants (CMRs) contribute directly to human monocyte activation in vitro, by increasing reactive oxygen species (ROS) production and cell migration. In this study, the effects of the oxidative state of CMR on the degree of monocyte activation was investigated. CMR-like particles (CRLPs)...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | International Journal of Vascular Medicine |
| Online Access: | http://dx.doi.org/10.1155/2012/942512 |
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| author | Sandra Armengol Lopez Kathleen M. Botham Charlotte Lawson |
| author_facet | Sandra Armengol Lopez Kathleen M. Botham Charlotte Lawson |
| author_sort | Sandra Armengol Lopez |
| collection | DOAJ |
| description | Chylomicron remnants (CMRs) contribute directly to human monocyte activation in vitro, by increasing reactive oxygen species (ROS) production and cell migration. In this study, the effects of the oxidative state of CMR on the degree of monocyte activation was investigated. CMR-like particles (CRLPs) were prepared in three different oxidative states, normal (CRLPs), protected from oxidation by incorporation of the antioxidant, probucol (pCRLPs), or oxidised with CuSO4 (oxCRLPs). Lipid accumulation and ROS production were significantly increased in primary human monocytes incubated with CRLPs, whilst secretion on monocyte chemoattractant protein-1 was reduced, but oxCRLPs had no additional effect. In contrast, pCRLPs were taken up by monocytes to a lesser extent and had no significant effect on ROS or MCP-1 secretion. These studies suggest that the oxidative state of CMRs modulates their stimulation of the activation of peripheral blood human monocytes and that dietary antioxidants may provide some protection against these atherogenic effects. |
| format | Article |
| id | doaj-art-b01afcb884ac47d8afc3e9034bfc57c6 |
| institution | Kabale University |
| issn | 2090-2824 2090-2832 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Vascular Medicine |
| spelling | doaj-art-b01afcb884ac47d8afc3e9034bfc57c62025-02-03T06:45:28ZengWileyInternational Journal of Vascular Medicine2090-28242090-28322012-01-01201210.1155/2012/942512942512The Oxidative State of Chylomicron Remnants Influences Their Modulation of Human Monocyte ActivationSandra Armengol Lopez0Kathleen M. Botham1Charlotte Lawson2Department of Physiology, Faculty of Medicine and Dentistry, University of the Basque Country, Sarriena s/n, 48940 Leioa, SpainDepartment of Veterinary Basic Sciences, The Royal Veterinary College, Royal College Street, London NW1 0TU, UKDepartment of Veterinary Basic Sciences, The Royal Veterinary College, Royal College Street, London NW1 0TU, UKChylomicron remnants (CMRs) contribute directly to human monocyte activation in vitro, by increasing reactive oxygen species (ROS) production and cell migration. In this study, the effects of the oxidative state of CMR on the degree of monocyte activation was investigated. CMR-like particles (CRLPs) were prepared in three different oxidative states, normal (CRLPs), protected from oxidation by incorporation of the antioxidant, probucol (pCRLPs), or oxidised with CuSO4 (oxCRLPs). Lipid accumulation and ROS production were significantly increased in primary human monocytes incubated with CRLPs, whilst secretion on monocyte chemoattractant protein-1 was reduced, but oxCRLPs had no additional effect. In contrast, pCRLPs were taken up by monocytes to a lesser extent and had no significant effect on ROS or MCP-1 secretion. These studies suggest that the oxidative state of CMRs modulates their stimulation of the activation of peripheral blood human monocytes and that dietary antioxidants may provide some protection against these atherogenic effects.http://dx.doi.org/10.1155/2012/942512 |
| spellingShingle | Sandra Armengol Lopez Kathleen M. Botham Charlotte Lawson The Oxidative State of Chylomicron Remnants Influences Their Modulation of Human Monocyte Activation International Journal of Vascular Medicine |
| title | The Oxidative State of Chylomicron Remnants Influences Their Modulation of Human Monocyte Activation |
| title_full | The Oxidative State of Chylomicron Remnants Influences Their Modulation of Human Monocyte Activation |
| title_fullStr | The Oxidative State of Chylomicron Remnants Influences Their Modulation of Human Monocyte Activation |
| title_full_unstemmed | The Oxidative State of Chylomicron Remnants Influences Their Modulation of Human Monocyte Activation |
| title_short | The Oxidative State of Chylomicron Remnants Influences Their Modulation of Human Monocyte Activation |
| title_sort | oxidative state of chylomicron remnants influences their modulation of human monocyte activation |
| url | http://dx.doi.org/10.1155/2012/942512 |
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