Circulating miR-323–3p as a novel potential plasma biomarker for multimorbidity burden and cognitive decline in middle-aged and older adults: Results from the national institute for longevity sciences–longitudinal study of aging in Japan

Background: Several circulating microRNAs (miRNAs) in the blood are indicators of chronic diseases, but their association with multimorbidity burden is unknown. The aim of this study was to assess the association of plasma levels of circulating miR-323–3p and miR-135–3p with age, cognitive performan...

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Main Authors: Wei-Min Chu, Mio Goto, Keiko Kabetani, Yukiko Nishita, Shu Zhang, Hiroshi Shimokata, Meng-Chih Lee, Akiko Satoh, Rei Otsuka
Format: Article
Language:English
Published: Elsevier 2024-12-01
Series:Archives of Gerontology and Geriatrics Plus
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Online Access:http://www.sciencedirect.com/science/article/pii/S2950307824000961
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author Wei-Min Chu
Mio Goto
Keiko Kabetani
Yukiko Nishita
Shu Zhang
Hiroshi Shimokata
Meng-Chih Lee
Akiko Satoh
Rei Otsuka
author_facet Wei-Min Chu
Mio Goto
Keiko Kabetani
Yukiko Nishita
Shu Zhang
Hiroshi Shimokata
Meng-Chih Lee
Akiko Satoh
Rei Otsuka
author_sort Wei-Min Chu
collection DOAJ
description Background: Several circulating microRNAs (miRNAs) in the blood are indicators of chronic diseases, but their association with multimorbidity burden is unknown. The aim of this study was to assess the association of plasma levels of circulating miR-323–3p and miR-135–3p with age, cognitive performance, and number of comorbidities in middle-aged and older individuals. Methods: Data from 295 community dwellers (≥40 years) who participated in the second wave (2000‒2002) of the National Institute for Longevity Sciences-Longitudinal Study of Aging in Japan were analyzed. miRNAs were isolated from the plasma, and miR-323–3p and miR-135–3p levels were measured using quantitative reverse-transcription polymerase chain reaction and normalized, with miR-16 as an endogenous reference gene. Cognitive performance was assessed using the Information and Similarities subtests and the Digit Symbol Substitution Test of the Wechsler Adult Intelligence Scale-Revised Short Form (WAIS-R-SF). Correlation tests and multivariate general linear regression analyses were performed to examine the relationship among circulating miRNA levels, burden of multimorbidity, and cognitive performance. Results: The mean age of the participants was 59.15 ± 10.26 years. Pearson's correlation analysis revealed that the miR-323–3p level positively correlated with age and number of comorbidities but negatively correlated with WAIS-R-SF subtest performance. In men, miR-323–3p level negatively correlated with the performance of all three WAIS-R-SF subtests. The general linear regression analysis showed that the miR-323–3p level increased in participants with four comorbidities compared with those with one comorbidity. Conclusion: Circulating miR-323–3p level is associated with the burden of multimorbidity and decreased cognitive performance in middle-aged and older adults.
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spelling doaj-art-b001cc3cb7944fe585b38237aea7d5212025-08-20T01:55:42ZengElsevierArchives of Gerontology and Geriatrics Plus2950-30782024-12-011410009910.1016/j.aggp.2024.100099Circulating miR-323–3p as a novel potential plasma biomarker for multimorbidity burden and cognitive decline in middle-aged and older adults: Results from the national institute for longevity sciences–longitudinal study of aging in JapanWei-Min Chu0Mio Goto1Keiko Kabetani2Yukiko Nishita3Shu Zhang4Hiroshi Shimokata5Meng-Chih Lee6Akiko Satoh7Rei Otsuka8Department of Family Medicine, Taichung Veterans General Hospital, Taichung, Taiwan; School of Medicine, National Yang Ming Chiao Tung University, Taipei, Taiwan; Department of Post‐Baccalaureate Medicine, College of Medicine, National Chung Hsing University, Taichung, Taiwan; Geriatrics and Gerontology Research Center, College of Medicine, National Chung Hsing University, Taichung, Taiwan; Department of Epidemiology of Aging, National Center for Geriatrics and Gerontology, Obu, Aichi, JapanDepartment of Integrative Physiology, Geroscience Research Center, National Center for Geriatrics and Gerontology, Obu, Aichi, JapanDepartment of Integrative Physiology, Geroscience Research Center, National Center for Geriatrics and Gerontology, Obu, Aichi, JapanDepartment of Epidemiology of Aging, National Center for Geriatrics and Gerontology, Obu, Aichi, JapanDepartment of Epidemiology of Aging, National Center for Geriatrics and Gerontology, Obu, Aichi, JapanDepartment of Epidemiology of Aging, National Center for Geriatrics and Gerontology, Obu, Aichi, Japan; Graduate School of Nutritional Sciences, Nagoya University of Arts and Sciences, Nisshin, Aichi, JapanDepartment of Family Medicine, Taichung Hospital, Ministry of Health and Welfare, Taichung, Taiwan; Institute of Population Sciences, National Health Research Institutes, Miaoli County, Taiwan; College of Management, Chaoyang University of Technology, Taichung, Taiwan; Institute of Medicine, Chung Shan Medical University, Taichung, TaiwanDepartment of Integrative Physiology, Geroscience Research Center, National Center for Geriatrics and Gerontology, Obu, Aichi, Japan; Department of Integrative Physiology, Institute of Development, Aging and Cancer, Tohoku University, Sendai, Japan; Corresponding author at: Department of Integrative Physiology, Geroscience Research Center, National Center for Geriatrics and Gerontology, 7-430 Morioka-cho, Obu, Aichi 474-8511, Japan.Department of Epidemiology of Aging, National Center for Geriatrics and Gerontology, Obu, Aichi, Japan; Corresponding author at: Department of Epidemiology of Aging, National Center for Geriatrics and Gerontology, 7-430 Morioka-cho, Obu, Aichi 474-8511, Japan.Background: Several circulating microRNAs (miRNAs) in the blood are indicators of chronic diseases, but their association with multimorbidity burden is unknown. The aim of this study was to assess the association of plasma levels of circulating miR-323–3p and miR-135–3p with age, cognitive performance, and number of comorbidities in middle-aged and older individuals. Methods: Data from 295 community dwellers (≥40 years) who participated in the second wave (2000‒2002) of the National Institute for Longevity Sciences-Longitudinal Study of Aging in Japan were analyzed. miRNAs were isolated from the plasma, and miR-323–3p and miR-135–3p levels were measured using quantitative reverse-transcription polymerase chain reaction and normalized, with miR-16 as an endogenous reference gene. Cognitive performance was assessed using the Information and Similarities subtests and the Digit Symbol Substitution Test of the Wechsler Adult Intelligence Scale-Revised Short Form (WAIS-R-SF). Correlation tests and multivariate general linear regression analyses were performed to examine the relationship among circulating miRNA levels, burden of multimorbidity, and cognitive performance. Results: The mean age of the participants was 59.15 ± 10.26 years. Pearson's correlation analysis revealed that the miR-323–3p level positively correlated with age and number of comorbidities but negatively correlated with WAIS-R-SF subtest performance. In men, miR-323–3p level negatively correlated with the performance of all three WAIS-R-SF subtests. The general linear regression analysis showed that the miR-323–3p level increased in participants with four comorbidities compared with those with one comorbidity. Conclusion: Circulating miR-323–3p level is associated with the burden of multimorbidity and decreased cognitive performance in middle-aged and older adults.http://www.sciencedirect.com/science/article/pii/S2950307824000961Circulating miRNAMultimorbidityChronic inflammationmiR-323–3pCognitive performance
spellingShingle Wei-Min Chu
Mio Goto
Keiko Kabetani
Yukiko Nishita
Shu Zhang
Hiroshi Shimokata
Meng-Chih Lee
Akiko Satoh
Rei Otsuka
Circulating miR-323–3p as a novel potential plasma biomarker for multimorbidity burden and cognitive decline in middle-aged and older adults: Results from the national institute for longevity sciences–longitudinal study of aging in Japan
Archives of Gerontology and Geriatrics Plus
Circulating miRNA
Multimorbidity
Chronic inflammation
miR-323–3p
Cognitive performance
title Circulating miR-323–3p as a novel potential plasma biomarker for multimorbidity burden and cognitive decline in middle-aged and older adults: Results from the national institute for longevity sciences–longitudinal study of aging in Japan
title_full Circulating miR-323–3p as a novel potential plasma biomarker for multimorbidity burden and cognitive decline in middle-aged and older adults: Results from the national institute for longevity sciences–longitudinal study of aging in Japan
title_fullStr Circulating miR-323–3p as a novel potential plasma biomarker for multimorbidity burden and cognitive decline in middle-aged and older adults: Results from the national institute for longevity sciences–longitudinal study of aging in Japan
title_full_unstemmed Circulating miR-323–3p as a novel potential plasma biomarker for multimorbidity burden and cognitive decline in middle-aged and older adults: Results from the national institute for longevity sciences–longitudinal study of aging in Japan
title_short Circulating miR-323–3p as a novel potential plasma biomarker for multimorbidity burden and cognitive decline in middle-aged and older adults: Results from the national institute for longevity sciences–longitudinal study of aging in Japan
title_sort circulating mir 323 3p as a novel potential plasma biomarker for multimorbidity burden and cognitive decline in middle aged and older adults results from the national institute for longevity sciences longitudinal study of aging in japan
topic Circulating miRNA
Multimorbidity
Chronic inflammation
miR-323–3p
Cognitive performance
url http://www.sciencedirect.com/science/article/pii/S2950307824000961
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