HLA Gene Polymorphisms in Romanian Patients with Chronic Lymphocytic Leukemia

Background and Objectives. Numerous genome-wide association studies have highlighted that chronic lymphocytic leukemia (CLL) is a lymphoproliferative disorder with an important genetic component. An important number of genes have been implicated in CLL etiology, with some of the most important being...

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Main Authors: Maria Tizu, Bogdan Calenic, Mihai Hârza, Bogdan M. Cristea, Ion Maruntelu, Andreea M. Caragea, Adriana Talangescu, Alina Dima, Alexandra E. Constantinescu, Ileana Constantinescu
Format: Article
Language:English
Published: Wiley 2024-01-01
Series:Genetics Research
Online Access:http://dx.doi.org/10.1155/2024/8852876
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author Maria Tizu
Bogdan Calenic
Mihai Hârza
Bogdan M. Cristea
Ion Maruntelu
Andreea M. Caragea
Adriana Talangescu
Alina Dima
Alexandra E. Constantinescu
Ileana Constantinescu
author_facet Maria Tizu
Bogdan Calenic
Mihai Hârza
Bogdan M. Cristea
Ion Maruntelu
Andreea M. Caragea
Adriana Talangescu
Alina Dima
Alexandra E. Constantinescu
Ileana Constantinescu
author_sort Maria Tizu
collection DOAJ
description Background and Objectives. Numerous genome-wide association studies have highlighted that chronic lymphocytic leukemia (CLL) is a lymphoproliferative disorder with an important genetic component. An important number of genes have been implicated in CLL etiology, with some of the most important being human leukocyte antigen (HLA) genes. The aim of this study was to analyze the association of CLL with certain HLA alleles in the Romanian population and to compare our results with previous findings. Materials and Methods. This study included 66 patients with CLL, diagnosed between 2020 and 2022, and 100 healthy controls. HLA class I and class II genes (HLA-A/B/C, HLA-DQA1/DQB1/DPA1/DPB1, and HLA-DRB1/3/4/5) were investigated using next-generation sequencing technology. Results. Several HLA alleles were strongly associated with CLL. The most important finding was that HLA-DRB1∗04:02:01 (p=0.001, OR = 1.05) and HLA-DRB3∗02:01:01 (p=0.009, OR = 1.03) have a predisposing role in CLL development. Moreover, we identified that HLA-A∗24:02:01 0.01 (p=0.01, OR = 0.38), HLA-DQA1∗05:05:01 (p=0.01, OR = 0.56), HLA-DQB1∗03:02:01 (p=0.03, OR = 0.40), and HLA-DRB4∗01:03:01 (p=0.03, OR = 0.54 alleles have protective roles. Correlations between HLA expression and gender showed that women had a higher expression of protective HLA alleles when compared to men. Conclusions. Our data are the first to indicate that in Romanian patients with CLL, the HLA-A∗24:02:01 and HLA-DQA1∗05:05:01 alleles have a protective role against CLL development, whereas HLA-DRB1∗04:02:01 and HLA-DRB3∗02:01:01alleles are positively associated with CLL.
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spelling doaj-art-afe916d7130b49af8277b3c5f14ed0ff2025-08-20T03:39:14ZengWileyGenetics Research1469-50732024-01-01202410.1155/2024/8852876HLA Gene Polymorphisms in Romanian Patients with Chronic Lymphocytic LeukemiaMaria Tizu0Bogdan Calenic1Mihai Hârza2Bogdan M. Cristea3Ion Maruntelu4Andreea M. Caragea5Adriana Talangescu6Alina Dima7Alexandra E. Constantinescu8Ileana Constantinescu9Immunology and Transplant ImmunologyImmunology and Transplant ImmunologyImmunology and Transplant ImmunologyImmunology and Transplant ImmunologyCentre of Immunogenetics and VirologyImmunology and Transplant ImmunologyCentre of Immunogenetics and VirologyColentina Clinical HospitalImmunology and Transplant ImmunologyImmunology and Transplant ImmunologyBackground and Objectives. Numerous genome-wide association studies have highlighted that chronic lymphocytic leukemia (CLL) is a lymphoproliferative disorder with an important genetic component. An important number of genes have been implicated in CLL etiology, with some of the most important being human leukocyte antigen (HLA) genes. The aim of this study was to analyze the association of CLL with certain HLA alleles in the Romanian population and to compare our results with previous findings. Materials and Methods. This study included 66 patients with CLL, diagnosed between 2020 and 2022, and 100 healthy controls. HLA class I and class II genes (HLA-A/B/C, HLA-DQA1/DQB1/DPA1/DPB1, and HLA-DRB1/3/4/5) were investigated using next-generation sequencing technology. Results. Several HLA alleles were strongly associated with CLL. The most important finding was that HLA-DRB1∗04:02:01 (p=0.001, OR = 1.05) and HLA-DRB3∗02:01:01 (p=0.009, OR = 1.03) have a predisposing role in CLL development. Moreover, we identified that HLA-A∗24:02:01 0.01 (p=0.01, OR = 0.38), HLA-DQA1∗05:05:01 (p=0.01, OR = 0.56), HLA-DQB1∗03:02:01 (p=0.03, OR = 0.40), and HLA-DRB4∗01:03:01 (p=0.03, OR = 0.54 alleles have protective roles. Correlations between HLA expression and gender showed that women had a higher expression of protective HLA alleles when compared to men. Conclusions. Our data are the first to indicate that in Romanian patients with CLL, the HLA-A∗24:02:01 and HLA-DQA1∗05:05:01 alleles have a protective role against CLL development, whereas HLA-DRB1∗04:02:01 and HLA-DRB3∗02:01:01alleles are positively associated with CLL.http://dx.doi.org/10.1155/2024/8852876
spellingShingle Maria Tizu
Bogdan Calenic
Mihai Hârza
Bogdan M. Cristea
Ion Maruntelu
Andreea M. Caragea
Adriana Talangescu
Alina Dima
Alexandra E. Constantinescu
Ileana Constantinescu
HLA Gene Polymorphisms in Romanian Patients with Chronic Lymphocytic Leukemia
Genetics Research
title HLA Gene Polymorphisms in Romanian Patients with Chronic Lymphocytic Leukemia
title_full HLA Gene Polymorphisms in Romanian Patients with Chronic Lymphocytic Leukemia
title_fullStr HLA Gene Polymorphisms in Romanian Patients with Chronic Lymphocytic Leukemia
title_full_unstemmed HLA Gene Polymorphisms in Romanian Patients with Chronic Lymphocytic Leukemia
title_short HLA Gene Polymorphisms in Romanian Patients with Chronic Lymphocytic Leukemia
title_sort hla gene polymorphisms in romanian patients with chronic lymphocytic leukemia
url http://dx.doi.org/10.1155/2024/8852876
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