Hepatoprotective effects of Curcuma xanthorrhiza Roxb. extract via free radical scavenger, inhibiting apoptosis and inflammation mechanisms in acetaminophen-induced liver injury
Objective(s): Acetaminophen (APAP)-mediated liver injury poses a significant public health concern. Curcuma xanthorrhiza extract (CXE) has been traditionally used for its hepatoprotective properties. This research aimed to assess the hepatoprotective effects of CXE in APAP-mediated hepatotoxicity by...
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| Language: | English |
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Mashhad University of Medical Sciences
2025-08-01
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| Series: | Iranian Journal of Basic Medical Sciences |
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| Online Access: | https://ijbms.mums.ac.ir/article_26052_0550e5c44fb86466578412e283237ff4.pdf |
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| author | I Nyoman Lister Linda Chiuman Maya Mutia Hartono Hartono Ermi Girsang Annisa Sutendi Hanna Kusuma Dhanar Hadiprasetyo Wahyu Widowati |
| author_facet | I Nyoman Lister Linda Chiuman Maya Mutia Hartono Hartono Ermi Girsang Annisa Sutendi Hanna Kusuma Dhanar Hadiprasetyo Wahyu Widowati |
| author_sort | I Nyoman Lister |
| collection | DOAJ |
| description | Objective(s): Acetaminophen (APAP)-mediated liver injury poses a significant public health concern. Curcuma xanthorrhiza extract (CXE) has been traditionally used for its hepatoprotective properties. This research aimed to assess the hepatoprotective effects of CXE in APAP-mediated hepatotoxicity by investigating the modulatory effects of CXE on key biomarkers, including Interleukin (IL), namely, (IL-6), IL-10, IL-1β, Nitric Oxide (NO), Lactate Dehydrogenase (LDH), and the genes expression related to apoptosis-like Caspase-3 (Casp-3), Casp-9, and genes related to liver metabolic c-Jun N-terminal Kinase (JNK), in APAP-mediated HepG2 cells.Materials and Methods: APAP-induced HepG2 cells were treated with different concentrations of CXE. IL-6, IL-10, IL were measured using an Enzyme-linked Immunosorbent Assay (ELISA) and NO, LDH were measured using colorimetric assay. Gene expression was analyzed using quantitative Real-Time Reverse Transcription (qRT-PCR).Results: CXE significantly reduced IL-1β and IL-6 levels, enhanced IL-10 production, and attenuated NO levels in APAP-mediated hepatotoxicity. CXE also suppressed the expression of Casp-9, Casp-3, JNK, and LDH levels. The study presented a concentration-dependent response, with 125 μg/ml CXE exhibiting the most pronounced effects. CXE effectively modulated immune responses, decreased oxidative stress, and inhibited apoptotic and inflammatory pathways in APAP-mediated hepatotoxic cells.Conclusion: These studies highlight the CXE potential as a therapeutic candidate for liver disorders, particularly in drug-mediated liver injury. |
| format | Article |
| id | doaj-art-afe08ea337724bb180397efc326c3ff1 |
| institution | Kabale University |
| issn | 2008-3866 2008-3874 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Basic Medical Sciences |
| spelling | doaj-art-afe08ea337724bb180397efc326c3ff12025-08-20T03:35:48ZengMashhad University of Medical SciencesIranian Journal of Basic Medical Sciences2008-38662008-38742025-08-012881100110610.22038/ijbms.2025.82500.1783326052Hepatoprotective effects of Curcuma xanthorrhiza Roxb. extract via free radical scavenger, inhibiting apoptosis and inflammation mechanisms in acetaminophen-induced liver injuryI Nyoman Lister0Linda Chiuman1Maya Mutia2Hartono Hartono3Ermi Girsang4Annisa Sutendi5Hanna Kusuma6Dhanar Hadiprasetyo7Wahyu Widowati8Faculty of Medicine, Universitas Prima Indonesia, Medan 20118, North Sumatera, IndonesiaFaculty of Medicine, Universitas Prima Indonesia, Medan 20118, North Sumatera, IndonesiaFaculty of Medicine, Universitas Prima Indonesia, Medan 20118, North Sumatera, IndonesiaFaculty of Medicine, Universitas Prima Indonesia, Medan 20118, North Sumatera, IndonesiaFaculty of Medicine, Universitas Prima Indonesia, Medan 20118, North Sumatera, IndonesiaBiomolecular and Biomedical Research Center, Aretha Medika Utama, Bandung 40163, IndonesiaBiomolecular and Biomedical Research Center, Aretha Medika Utama, Bandung 40163, IndonesiaBiomolecular and Biomedical Research Center, Aretha Medika Utama, Bandung 40163, IndonesiaFaculty of Medicine, Maranatha Christian University, Bandung 40164, West Java, IndonesiaObjective(s): Acetaminophen (APAP)-mediated liver injury poses a significant public health concern. Curcuma xanthorrhiza extract (CXE) has been traditionally used for its hepatoprotective properties. This research aimed to assess the hepatoprotective effects of CXE in APAP-mediated hepatotoxicity by investigating the modulatory effects of CXE on key biomarkers, including Interleukin (IL), namely, (IL-6), IL-10, IL-1β, Nitric Oxide (NO), Lactate Dehydrogenase (LDH), and the genes expression related to apoptosis-like Caspase-3 (Casp-3), Casp-9, and genes related to liver metabolic c-Jun N-terminal Kinase (JNK), in APAP-mediated HepG2 cells.Materials and Methods: APAP-induced HepG2 cells were treated with different concentrations of CXE. IL-6, IL-10, IL were measured using an Enzyme-linked Immunosorbent Assay (ELISA) and NO, LDH were measured using colorimetric assay. Gene expression was analyzed using quantitative Real-Time Reverse Transcription (qRT-PCR).Results: CXE significantly reduced IL-1β and IL-6 levels, enhanced IL-10 production, and attenuated NO levels in APAP-mediated hepatotoxicity. CXE also suppressed the expression of Casp-9, Casp-3, JNK, and LDH levels. The study presented a concentration-dependent response, with 125 μg/ml CXE exhibiting the most pronounced effects. CXE effectively modulated immune responses, decreased oxidative stress, and inhibited apoptotic and inflammatory pathways in APAP-mediated hepatotoxic cells.Conclusion: These studies highlight the CXE potential as a therapeutic candidate for liver disorders, particularly in drug-mediated liver injury.https://ijbms.mums.ac.ir/article_26052_0550e5c44fb86466578412e283237ff4.pdfacetaminophenantioxidantsapoptosiscurcuma xanthorrhizafree radical scavengers liver injury |
| spellingShingle | I Nyoman Lister Linda Chiuman Maya Mutia Hartono Hartono Ermi Girsang Annisa Sutendi Hanna Kusuma Dhanar Hadiprasetyo Wahyu Widowati Hepatoprotective effects of Curcuma xanthorrhiza Roxb. extract via free radical scavenger, inhibiting apoptosis and inflammation mechanisms in acetaminophen-induced liver injury Iranian Journal of Basic Medical Sciences acetaminophen antioxidants apoptosis curcuma xanthorrhiza free radical scavengers liver injury |
| title | Hepatoprotective effects of Curcuma xanthorrhiza Roxb. extract via free radical scavenger, inhibiting apoptosis and inflammation mechanisms in acetaminophen-induced liver injury |
| title_full | Hepatoprotective effects of Curcuma xanthorrhiza Roxb. extract via free radical scavenger, inhibiting apoptosis and inflammation mechanisms in acetaminophen-induced liver injury |
| title_fullStr | Hepatoprotective effects of Curcuma xanthorrhiza Roxb. extract via free radical scavenger, inhibiting apoptosis and inflammation mechanisms in acetaminophen-induced liver injury |
| title_full_unstemmed | Hepatoprotective effects of Curcuma xanthorrhiza Roxb. extract via free radical scavenger, inhibiting apoptosis and inflammation mechanisms in acetaminophen-induced liver injury |
| title_short | Hepatoprotective effects of Curcuma xanthorrhiza Roxb. extract via free radical scavenger, inhibiting apoptosis and inflammation mechanisms in acetaminophen-induced liver injury |
| title_sort | hepatoprotective effects of curcuma xanthorrhiza roxb extract via free radical scavenger inhibiting apoptosis and inflammation mechanisms in acetaminophen induced liver injury |
| topic | acetaminophen antioxidants apoptosis curcuma xanthorrhiza free radical scavengers liver injury |
| url | https://ijbms.mums.ac.ir/article_26052_0550e5c44fb86466578412e283237ff4.pdf |
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