GENOTYPE -786CC OF THE ENDOTHELIAL NITRIC OXIDE SYNTHASE GENE NOS3 AS A FACTOR OF ADVERSE CORONARY HEART DISEASE COURSE AND INCREASED ON-TREATMENT PLATELET AGGREGATION

Aim. To assess the associations of polymorphism T-786C gene NOS3 with the severity of clinical course of coronary heart disease (CHD) and platelet aggregatability in the selection of patients receiving clopidogrel and acetylsalicylic acid (ASA) compounds after selective coronary intervention.Materia...

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Main Authors: E. F. Muslimova, T. Yu. Rebrova, S. A. Afanasiev, T. N. Sergienko, A. N. Repin
Format: Article
Language:Russian
Published: «FIRMA «SILICEA» LLC 2017-11-01
Series:Российский кардиологический журнал
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Online Access:https://russjcardiol.elpub.ru/jour/article/view/1845
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author E. F. Muslimova
T. Yu. Rebrova
S. A. Afanasiev
T. N. Sergienko
A. N. Repin
author_facet E. F. Muslimova
T. Yu. Rebrova
S. A. Afanasiev
T. N. Sergienko
A. N. Repin
author_sort E. F. Muslimova
collection DOAJ
description Aim. To assess the associations of polymorphism T-786C gene NOS3 with the severity of clinical course of coronary heart disease (CHD) and platelet aggregatability in the selection of patients receiving clopidogrel and acetylsalicylic acid (ASA) compounds after selective coronary intervention.Material and methods. In the study, 203 CHD males included, taking ASA and clopidogrel as double antiplatelet therapy for coronary intervention. The test performed, of induced platelet aggregation with adenosine diphosphate (ADP) (2,5 mcM and 5,0 mcM) and epinephrine (0,2 mcM). Genotyping was done with the allele specific polymerase chain reaction (“SNP-express”, SPF Litekh, Russia). Statistics was done with Mann-Whitney test, Kruskal-Wallis test and Pearson chisquare or bi-test by Fisher. Differences were taken as significant at p<0,05.Results. In the studied group, genotypes -786TT, -786TC, -786CC were found with the prevalences 72 (35,4%), 99 (48,8%), 32 (15,8%), respectively. For the carriers of -786CC there was found highest grade of platelet aggregation with ADP 2,5 mcM (p=0,047) and with epinephrine (p=0,008). Carriers of -786TC had the highest left ventricle ejection fraction (p=0,035).Conclusion. In the selection of CHD males taking ASA and clopidogrel, carriage of -786CC polymorphism T-786C gene NOS3 was related to higher platelet aggregation in response to ADP and epinephrine. For these patients, the carriage of genotype -786CC gene NOS3 might be a predictor of thrombotic complications after coronary stenting and more adverse outcome of CHD.
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issn 1560-4071
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spelling doaj-art-afda0b9d1117423bb01bbe0a156cdf522025-08-20T03:57:26Zrus«FIRMA «SILICEA» LLCРоссийский кардиологический журнал1560-40712618-76202017-11-01010293210.15829/1560-4071-2017-10-29-322068GENOTYPE -786CC OF THE ENDOTHELIAL NITRIC OXIDE SYNTHASE GENE NOS3 AS A FACTOR OF ADVERSE CORONARY HEART DISEASE COURSE AND INCREASED ON-TREATMENT PLATELET AGGREGATIONE. F. Muslimova0T. Yu. Rebrova1S. A. Afanasiev2T. N. Sergienko3A. N. Repin4Cardiology Research Institute, Tomsk National Research Medical Center, Russian Academy of SciencesCardiology Research Institute, Tomsk National Research Medical Center, Russian Academy of SciencesCardiology Research Institute, Tomsk National Research Medical Center, Russian Academy of SciencesCardiology Research Institute, Tomsk National Research Medical Center, Russian Academy of SciencesCardiology Research Institute, Tomsk National Research Medical Center, Russian Academy of SciencesAim. To assess the associations of polymorphism T-786C gene NOS3 with the severity of clinical course of coronary heart disease (CHD) and platelet aggregatability in the selection of patients receiving clopidogrel and acetylsalicylic acid (ASA) compounds after selective coronary intervention.Material and methods. In the study, 203 CHD males included, taking ASA and clopidogrel as double antiplatelet therapy for coronary intervention. The test performed, of induced platelet aggregation with adenosine diphosphate (ADP) (2,5 mcM and 5,0 mcM) and epinephrine (0,2 mcM). Genotyping was done with the allele specific polymerase chain reaction (“SNP-express”, SPF Litekh, Russia). Statistics was done with Mann-Whitney test, Kruskal-Wallis test and Pearson chisquare or bi-test by Fisher. Differences were taken as significant at p<0,05.Results. In the studied group, genotypes -786TT, -786TC, -786CC were found with the prevalences 72 (35,4%), 99 (48,8%), 32 (15,8%), respectively. For the carriers of -786CC there was found highest grade of platelet aggregation with ADP 2,5 mcM (p=0,047) and with epinephrine (p=0,008). Carriers of -786TC had the highest left ventricle ejection fraction (p=0,035).Conclusion. In the selection of CHD males taking ASA and clopidogrel, carriage of -786CC polymorphism T-786C gene NOS3 was related to higher platelet aggregation in response to ADP and epinephrine. For these patients, the carriage of genotype -786CC gene NOS3 might be a predictor of thrombotic complications after coronary stenting and more adverse outcome of CHD.https://russjcardiol.elpub.ru/jour/article/view/1845polymorphismnos3chdplatelet aggregation
spellingShingle E. F. Muslimova
T. Yu. Rebrova
S. A. Afanasiev
T. N. Sergienko
A. N. Repin
GENOTYPE -786CC OF THE ENDOTHELIAL NITRIC OXIDE SYNTHASE GENE NOS3 AS A FACTOR OF ADVERSE CORONARY HEART DISEASE COURSE AND INCREASED ON-TREATMENT PLATELET AGGREGATION
Российский кардиологический журнал
polymorphism
nos3
chd
platelet aggregation
title GENOTYPE -786CC OF THE ENDOTHELIAL NITRIC OXIDE SYNTHASE GENE NOS3 AS A FACTOR OF ADVERSE CORONARY HEART DISEASE COURSE AND INCREASED ON-TREATMENT PLATELET AGGREGATION
title_full GENOTYPE -786CC OF THE ENDOTHELIAL NITRIC OXIDE SYNTHASE GENE NOS3 AS A FACTOR OF ADVERSE CORONARY HEART DISEASE COURSE AND INCREASED ON-TREATMENT PLATELET AGGREGATION
title_fullStr GENOTYPE -786CC OF THE ENDOTHELIAL NITRIC OXIDE SYNTHASE GENE NOS3 AS A FACTOR OF ADVERSE CORONARY HEART DISEASE COURSE AND INCREASED ON-TREATMENT PLATELET AGGREGATION
title_full_unstemmed GENOTYPE -786CC OF THE ENDOTHELIAL NITRIC OXIDE SYNTHASE GENE NOS3 AS A FACTOR OF ADVERSE CORONARY HEART DISEASE COURSE AND INCREASED ON-TREATMENT PLATELET AGGREGATION
title_short GENOTYPE -786CC OF THE ENDOTHELIAL NITRIC OXIDE SYNTHASE GENE NOS3 AS A FACTOR OF ADVERSE CORONARY HEART DISEASE COURSE AND INCREASED ON-TREATMENT PLATELET AGGREGATION
title_sort genotype 786cc of the endothelial nitric oxide synthase gene nos3 as a factor of adverse coronary heart disease course and increased on treatment platelet aggregation
topic polymorphism
nos3
chd
platelet aggregation
url https://russjcardiol.elpub.ru/jour/article/view/1845
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AT tyurebrova genotype786ccoftheendothelialnitricoxidesynthasegenenos3asafactorofadversecoronaryheartdiseasecourseandincreasedontreatmentplateletaggregation
AT saafanasiev genotype786ccoftheendothelialnitricoxidesynthasegenenos3asafactorofadversecoronaryheartdiseasecourseandincreasedontreatmentplateletaggregation
AT tnsergienko genotype786ccoftheendothelialnitricoxidesynthasegenenos3asafactorofadversecoronaryheartdiseasecourseandincreasedontreatmentplateletaggregation
AT anrepin genotype786ccoftheendothelialnitricoxidesynthasegenenos3asafactorofadversecoronaryheartdiseasecourseandincreasedontreatmentplateletaggregation