Serum Tau Species in Progressive Supranuclear Palsy: A Pilot Study
Background/Objectives: Progressive Supranuclear Palsy (PSP) is a tauopathy showing a marked symptoms overlap with Parkinson’s Disease (PD). PSP pathology suggests that tau protein might represent a valuable biomarker to distinguish between the two diseases. Here, we investigated the presence and dia...
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MDPI AG
2024-12-01
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| author | Costanza Maria Cristiani Luana Scaramuzzino Elvira Immacolata Parrotta Giovanni Cuda Aldo Quattrone Andrea Quattrone |
| author_facet | Costanza Maria Cristiani Luana Scaramuzzino Elvira Immacolata Parrotta Giovanni Cuda Aldo Quattrone Andrea Quattrone |
| author_sort | Costanza Maria Cristiani |
| collection | DOAJ |
| description | Background/Objectives: Progressive Supranuclear Palsy (PSP) is a tauopathy showing a marked symptoms overlap with Parkinson’s Disease (PD). PSP pathology suggests that tau protein might represent a valuable biomarker to distinguish between the two diseases. Here, we investigated the presence and diagnostic value of six different tau species (total tau, 4R-tau isoform, tau aggregates, p-tau202, p-tau231 and p-tau396) in serum from 13 PSP and 13 PD patients and 12 healthy controls (HCs). Methods: ELISA commercial kits were employed to assess all the tau species except for t-tau, which was assessed by a single molecule array (SIMOA)-based commercial kit. Possible correlations between tau species and biological and clinical features of our cohorts were also evaluated. Results: Among the six tau species tested, only p-tau396 was detectable in serum. Concentration of p-tau396 was significantly higher in both PSP and PD groups compared to HC, but PSP and PD patients showed largely overlapping values. Moreover, serum concentration of p-tau396 strongly correlated with disease severity in PSP and not in PD. Conclusions: Overall, we identified serum p-tau396 as the most expressed phosphorylated tau species in serum and as a potential tool for assessing PSP clinical staging. Moreover, we demonstrated that other p-tau species may be present at too low concentrations in serum to be detected by ELISA, suggesting that future work should focus on other biological matrices. |
| format | Article |
| id | doaj-art-afd955adaca34227b8a04c87263a40e5 |
| institution | OA Journals |
| issn | 2075-4418 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
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| spelling | doaj-art-afd955adaca34227b8a04c87263a40e52025-08-20T02:38:43ZengMDPI AGDiagnostics2075-44182024-12-011423274610.3390/diagnostics14232746Serum Tau Species in Progressive Supranuclear Palsy: A Pilot StudyCostanza Maria Cristiani0Luana Scaramuzzino1Elvira Immacolata Parrotta2Giovanni Cuda3Aldo Quattrone4Andrea Quattrone5Neuroscience Research Center, Department of Medical and Surgical Sciences, University “Magna Graecia”, 88100 Catanzaro, ItalyNeuroscience Research Center, Department of Medical and Surgical Sciences, University “Magna Graecia”, 88100 Catanzaro, ItalyInstitute of Molecular Biology, Department of Medical and Surgical Sciences, University “Magna Graecia”, 88100 Catanzaro, ItalyResearch Centre for Advanced Biochemistry and Molecular Biology, Department of Clinical and Experimental Medicine, University “Magna Graecia”, 88100 Catanzaro, ItalyNeuroscience Research Center, Department of Medical and Surgical Sciences, University “Magna Graecia”, 88100 Catanzaro, ItalyNeuroscience Research Center, Department of Medical and Surgical Sciences, University “Magna Graecia”, 88100 Catanzaro, ItalyBackground/Objectives: Progressive Supranuclear Palsy (PSP) is a tauopathy showing a marked symptoms overlap with Parkinson’s Disease (PD). PSP pathology suggests that tau protein might represent a valuable biomarker to distinguish between the two diseases. Here, we investigated the presence and diagnostic value of six different tau species (total tau, 4R-tau isoform, tau aggregates, p-tau202, p-tau231 and p-tau396) in serum from 13 PSP and 13 PD patients and 12 healthy controls (HCs). Methods: ELISA commercial kits were employed to assess all the tau species except for t-tau, which was assessed by a single molecule array (SIMOA)-based commercial kit. Possible correlations between tau species and biological and clinical features of our cohorts were also evaluated. Results: Among the six tau species tested, only p-tau396 was detectable in serum. Concentration of p-tau396 was significantly higher in both PSP and PD groups compared to HC, but PSP and PD patients showed largely overlapping values. Moreover, serum concentration of p-tau396 strongly correlated with disease severity in PSP and not in PD. Conclusions: Overall, we identified serum p-tau396 as the most expressed phosphorylated tau species in serum and as a potential tool for assessing PSP clinical staging. Moreover, we demonstrated that other p-tau species may be present at too low concentrations in serum to be detected by ELISA, suggesting that future work should focus on other biological matrices.https://www.mdpi.com/2075-4418/14/23/2746progressive supranuclear palsyParkinson’s diseaseELISAp-tau396serum |
| spellingShingle | Costanza Maria Cristiani Luana Scaramuzzino Elvira Immacolata Parrotta Giovanni Cuda Aldo Quattrone Andrea Quattrone Serum Tau Species in Progressive Supranuclear Palsy: A Pilot Study Diagnostics progressive supranuclear palsy Parkinson’s disease ELISA p-tau396 serum |
| title | Serum Tau Species in Progressive Supranuclear Palsy: A Pilot Study |
| title_full | Serum Tau Species in Progressive Supranuclear Palsy: A Pilot Study |
| title_fullStr | Serum Tau Species in Progressive Supranuclear Palsy: A Pilot Study |
| title_full_unstemmed | Serum Tau Species in Progressive Supranuclear Palsy: A Pilot Study |
| title_short | Serum Tau Species in Progressive Supranuclear Palsy: A Pilot Study |
| title_sort | serum tau species in progressive supranuclear palsy a pilot study |
| topic | progressive supranuclear palsy Parkinson’s disease ELISA p-tau396 serum |
| url | https://www.mdpi.com/2075-4418/14/23/2746 |
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