Expression of a bee-venom phospholipase A<sub>2</sub> gene from Apis mellifera in Escherichia coli
The venomous phospholipase A<sub>2</sub> (AmPLA<sub>2</sub>) coding reading region of Italian honeybee (Apis mellifera), which was found to be composed of 405 bp, was inserted into the expression vector pET-blue-1. Then the recombinant vector was introduced into Escherichia c...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Zhejiang University Press
2004-01-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
| Subjects: | |
| Online Access: | https://www.academax.com/doi/10.3785/1008-9209.2004.01.0089 |
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| Summary: | The venomous phospholipase A<sub>2</sub> (AmPLA<sub>2</sub>) coding reading region of Italian honeybee (Apis mellifera), which was found to be composed of 405 bp, was inserted into the expression vector pET-blue-1. Then the recombinant vector was introduced into Escherichia coli Tuner (DE3) plac I for expression. Analysis of the result of SDS-PAGE showed that the expression products contained a band of protein of about 15 kD. Detection of western blot using ant-AmPLA<sub>2</sub> polyclonal serum of rabbit as the first antibody showed that a special blot with the some characteristics as the native AmPLA<sub>2</sub> was revealed among the expression products. This was considered as the evidence for successful expression of AmPLA<sub>2</sub> in E. coli. |
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| ISSN: | 1008-9209 2097-5155 |