Chromosome-level genome assembly and annotation of Anthurium amnicola

Abstract Anthurium amnicola is in the monocot family Araceae, subfamily Pothoideae and is a contributing species in Hawaii floriculture industry hybrids. To support future genetic improvements to this commodity, we sequenced and assembled the A. amnicola genome to chromosome-scale using PacBio HiFi...

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Main Authors: Haomin Lyu, Sheina B. Sim, Scott M. Geib, Joanne S. L. Imamura, Briette L. Corpuz, Renee L. Corpuz, Angela N. Kauwe, Tyler J. Simmonds, Claire N. Arakawa, Roxana Y. Myers, Lisa M. Keith, Qingyi Yu, Tracie K. Matsumoto, Teresita D. Amore, Jon Y. Suzuki
Format: Article
Language:English
Published: Nature Portfolio 2025-04-01
Series:Scientific Data
Online Access:https://doi.org/10.1038/s41597-025-04939-4
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author Haomin Lyu
Sheina B. Sim
Scott M. Geib
Joanne S. L. Imamura
Briette L. Corpuz
Renee L. Corpuz
Angela N. Kauwe
Tyler J. Simmonds
Claire N. Arakawa
Roxana Y. Myers
Lisa M. Keith
Qingyi Yu
Tracie K. Matsumoto
Teresita D. Amore
Jon Y. Suzuki
author_facet Haomin Lyu
Sheina B. Sim
Scott M. Geib
Joanne S. L. Imamura
Briette L. Corpuz
Renee L. Corpuz
Angela N. Kauwe
Tyler J. Simmonds
Claire N. Arakawa
Roxana Y. Myers
Lisa M. Keith
Qingyi Yu
Tracie K. Matsumoto
Teresita D. Amore
Jon Y. Suzuki
author_sort Haomin Lyu
collection DOAJ
description Abstract Anthurium amnicola is in the monocot family Araceae, subfamily Pothoideae and is a contributing species in Hawaii floriculture industry hybrids. To support future genetic improvements to this commodity, we sequenced and assembled the A. amnicola genome to chromosome-scale using PacBio HiFi and short-read Hi-C sequencing. A total of 98.51% of the 4.79 Gb genome is anchored into 15 chromosomes, with 99.2% gene completeness and a high LTR assembly index (LAI) score of 21.73, indicative of a complete, high-quality assembly. Annotation reveals the presence of 20,380 protein-coding genes, with 78.52% of the genome composed of repetitive sequences, predominantly long terminal repeat retrotransposons (LTR-RT). Phylogenetic analysis identified evolutionary relationships between A. amnicola and representative species in the Araceae and other plant families. This study provides the first reference genome sequence for the neotropical genus Anthurium and insights into Araceae evolution, benefiting the floriculture industry and evolutionary studies.
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spelling doaj-art-afd680c399e04fdd9cc388b31d1ea4882025-08-20T02:11:51ZengNature PortfolioScientific Data2052-44632025-04-011211810.1038/s41597-025-04939-4Chromosome-level genome assembly and annotation of Anthurium amnicolaHaomin Lyu0Sheina B. Sim1Scott M. Geib2Joanne S. L. Imamura3Briette L. Corpuz4Renee L. Corpuz5Angela N. Kauwe6Tyler J. Simmonds7Claire N. Arakawa8Roxana Y. Myers9Lisa M. Keith10Qingyi Yu11Tracie K. Matsumoto12Teresita D. Amore13Jon Y. Suzuki14USDA ARS Daniel K. Inouye, U.S. Pacific Basin Agricultural Research CenterUSDA ARS Daniel K. Inouye, U.S. Pacific Basin Agricultural Research CenterUSDA ARS Daniel K. Inouye, U.S. Pacific Basin Agricultural Research CenterDepartment of Tropical Plant and Soil Sciences, University of Hawaii at ManoaUSDA ARS Daniel K. Inouye, U.S. Pacific Basin Agricultural Research CenterUSDA ARS Daniel K. Inouye, U.S. Pacific Basin Agricultural Research CenterUSDA ARS Daniel K. Inouye, U.S. Pacific Basin Agricultural Research CenterUSDA ARS Daniel K. Inouye, U.S. Pacific Basin Agricultural Research CenterUSDA ARS Daniel K. Inouye, U.S. Pacific Basin Agricultural Research CenterUSDA ARS Daniel K. Inouye, U.S. Pacific Basin Agricultural Research CenterUSDA ARS Daniel K. Inouye, U.S. Pacific Basin Agricultural Research CenterUSDA ARS Daniel K. Inouye, U.S. Pacific Basin Agricultural Research CenterUSDA ARS Daniel K. Inouye, U.S. Pacific Basin Agricultural Research CenterDepartment of Tropical Plant and Soil Sciences, University of Hawaii at ManoaUSDA ARS Daniel K. Inouye, U.S. Pacific Basin Agricultural Research CenterAbstract Anthurium amnicola is in the monocot family Araceae, subfamily Pothoideae and is a contributing species in Hawaii floriculture industry hybrids. To support future genetic improvements to this commodity, we sequenced and assembled the A. amnicola genome to chromosome-scale using PacBio HiFi and short-read Hi-C sequencing. A total of 98.51% of the 4.79 Gb genome is anchored into 15 chromosomes, with 99.2% gene completeness and a high LTR assembly index (LAI) score of 21.73, indicative of a complete, high-quality assembly. Annotation reveals the presence of 20,380 protein-coding genes, with 78.52% of the genome composed of repetitive sequences, predominantly long terminal repeat retrotransposons (LTR-RT). Phylogenetic analysis identified evolutionary relationships between A. amnicola and representative species in the Araceae and other plant families. This study provides the first reference genome sequence for the neotropical genus Anthurium and insights into Araceae evolution, benefiting the floriculture industry and evolutionary studies.https://doi.org/10.1038/s41597-025-04939-4
spellingShingle Haomin Lyu
Sheina B. Sim
Scott M. Geib
Joanne S. L. Imamura
Briette L. Corpuz
Renee L. Corpuz
Angela N. Kauwe
Tyler J. Simmonds
Claire N. Arakawa
Roxana Y. Myers
Lisa M. Keith
Qingyi Yu
Tracie K. Matsumoto
Teresita D. Amore
Jon Y. Suzuki
Chromosome-level genome assembly and annotation of Anthurium amnicola
Scientific Data
title Chromosome-level genome assembly and annotation of Anthurium amnicola
title_full Chromosome-level genome assembly and annotation of Anthurium amnicola
title_fullStr Chromosome-level genome assembly and annotation of Anthurium amnicola
title_full_unstemmed Chromosome-level genome assembly and annotation of Anthurium amnicola
title_short Chromosome-level genome assembly and annotation of Anthurium amnicola
title_sort chromosome level genome assembly and annotation of anthurium amnicola
url https://doi.org/10.1038/s41597-025-04939-4
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