Mesenchymal Stem Cell-Educated Macrophages Ameliorate LPS-Induced Systemic Response
Both bone marrow and adipose-derived mesenchymal stem cells (ASCs) have immunomodulatory effects. The goal of this study was to determine whether ASCs-educated macrophages could directly ameliorate LPS-induced systemic response in a mouse model. Mouse peritoneal macrophages were cocultured with ASCs...
Saved in:
| Main Authors: | , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2016-01-01
|
| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2016/3735452 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832561789122904064 |
|---|---|
| author | Yaoqin Hu Chaojin Qin Guoping Zheng Dengming Lai Huikang Tao Yan Zhang Guanguan Qiu Menghua Ge Lanfang Huang Lina Chen Baoli Cheng Qiang Shu Jianguo Xu |
| author_facet | Yaoqin Hu Chaojin Qin Guoping Zheng Dengming Lai Huikang Tao Yan Zhang Guanguan Qiu Menghua Ge Lanfang Huang Lina Chen Baoli Cheng Qiang Shu Jianguo Xu |
| author_sort | Yaoqin Hu |
| collection | DOAJ |
| description | Both bone marrow and adipose-derived mesenchymal stem cells (ASCs) have immunomodulatory effects. The goal of this study was to determine whether ASCs-educated macrophages could directly ameliorate LPS-induced systemic response in a mouse model. Mouse peritoneal macrophages were cocultured with ASCs in a Transwell system for 2 days to educate macrophages. Mice were divided into 5 groups: control, LPS, LPS + ASCs, LPS + untreated macrophages, and LPS + educated macrophages. Educated macrophages decreased lung inflammation, weight loss, pulmonary edema, and inflammatory cytokine response. In vitro, ASCs increased expression of M2 macrophages independent of direct cell-to-cell contact when macrophages were treated with LPS or serum from patients with acute respiratory distress syndrome (ARDS). When macrophages were cultured with serum from ARDS patients who were treated with ASCs or placebo in our previous clinical trial, there was no difference in M2 macrophage levels before and after ASCs treatment indicating a suboptimal response to the treatment protocol. ASCs also reduced the levels of LPS-induced proinflammatory cytokines in vitro which were mimicked by IL-10 and blocked by antibodies for IL-10 and IL-10 receptor supporting the notion that educated macrophages exert their anti-inflammatory effects via IL-10-dependent mechanisms. |
| format | Article |
| id | doaj-art-afd0937fef3948abb76f819be2bd4e2f |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-afd0937fef3948abb76f819be2bd4e2f2025-02-03T01:24:18ZengWileyMediators of Inflammation0962-93511466-18612016-01-01201610.1155/2016/37354523735452Mesenchymal Stem Cell-Educated Macrophages Ameliorate LPS-Induced Systemic ResponseYaoqin Hu0Chaojin Qin1Guoping Zheng2Dengming Lai3Huikang Tao4Yan Zhang5Guanguan Qiu6Menghua Ge7Lanfang Huang8Lina Chen9Baoli Cheng10Qiang Shu11Jianguo Xu12The Children’s Hospital of Zhejiang University School of Medicine, Hangzhou, Zhejiang 310052, ChinaThe Children’s Hospital of Zhejiang University School of Medicine, Hangzhou, Zhejiang 310052, ChinaShaoxing Second Hospital, Shaoxing, Zhejiang 312000, ChinaThe Children’s Hospital of Zhejiang University School of Medicine, Hangzhou, Zhejiang 310052, ChinaThe Children’s Hospital of Zhejiang University School of Medicine, Hangzhou, Zhejiang 310052, ChinaThe Children’s Hospital of Zhejiang University School of Medicine, Hangzhou, Zhejiang 310052, ChinaShaoxing Second Hospital, Shaoxing, Zhejiang 312000, ChinaShaoxing Second Hospital, Shaoxing, Zhejiang 312000, ChinaShaoxing Second Hospital, Shaoxing, Zhejiang 312000, ChinaShaoxing Second Hospital, Shaoxing, Zhejiang 312000, ChinaThe First Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, Zhejiang 310003, ChinaThe Children’s Hospital of Zhejiang University School of Medicine, Hangzhou, Zhejiang 310052, ChinaShaoxing Second Hospital, Shaoxing, Zhejiang 312000, ChinaBoth bone marrow and adipose-derived mesenchymal stem cells (ASCs) have immunomodulatory effects. The goal of this study was to determine whether ASCs-educated macrophages could directly ameliorate LPS-induced systemic response in a mouse model. Mouse peritoneal macrophages were cocultured with ASCs in a Transwell system for 2 days to educate macrophages. Mice were divided into 5 groups: control, LPS, LPS + ASCs, LPS + untreated macrophages, and LPS + educated macrophages. Educated macrophages decreased lung inflammation, weight loss, pulmonary edema, and inflammatory cytokine response. In vitro, ASCs increased expression of M2 macrophages independent of direct cell-to-cell contact when macrophages were treated with LPS or serum from patients with acute respiratory distress syndrome (ARDS). When macrophages were cultured with serum from ARDS patients who were treated with ASCs or placebo in our previous clinical trial, there was no difference in M2 macrophage levels before and after ASCs treatment indicating a suboptimal response to the treatment protocol. ASCs also reduced the levels of LPS-induced proinflammatory cytokines in vitro which were mimicked by IL-10 and blocked by antibodies for IL-10 and IL-10 receptor supporting the notion that educated macrophages exert their anti-inflammatory effects via IL-10-dependent mechanisms.http://dx.doi.org/10.1155/2016/3735452 |
| spellingShingle | Yaoqin Hu Chaojin Qin Guoping Zheng Dengming Lai Huikang Tao Yan Zhang Guanguan Qiu Menghua Ge Lanfang Huang Lina Chen Baoli Cheng Qiang Shu Jianguo Xu Mesenchymal Stem Cell-Educated Macrophages Ameliorate LPS-Induced Systemic Response Mediators of Inflammation |
| title | Mesenchymal Stem Cell-Educated Macrophages Ameliorate LPS-Induced Systemic Response |
| title_full | Mesenchymal Stem Cell-Educated Macrophages Ameliorate LPS-Induced Systemic Response |
| title_fullStr | Mesenchymal Stem Cell-Educated Macrophages Ameliorate LPS-Induced Systemic Response |
| title_full_unstemmed | Mesenchymal Stem Cell-Educated Macrophages Ameliorate LPS-Induced Systemic Response |
| title_short | Mesenchymal Stem Cell-Educated Macrophages Ameliorate LPS-Induced Systemic Response |
| title_sort | mesenchymal stem cell educated macrophages ameliorate lps induced systemic response |
| url | http://dx.doi.org/10.1155/2016/3735452 |
| work_keys_str_mv | AT yaoqinhu mesenchymalstemcelleducatedmacrophagesamelioratelpsinducedsystemicresponse AT chaojinqin mesenchymalstemcelleducatedmacrophagesamelioratelpsinducedsystemicresponse AT guopingzheng mesenchymalstemcelleducatedmacrophagesamelioratelpsinducedsystemicresponse AT dengminglai mesenchymalstemcelleducatedmacrophagesamelioratelpsinducedsystemicresponse AT huikangtao mesenchymalstemcelleducatedmacrophagesamelioratelpsinducedsystemicresponse AT yanzhang mesenchymalstemcelleducatedmacrophagesamelioratelpsinducedsystemicresponse AT guanguanqiu mesenchymalstemcelleducatedmacrophagesamelioratelpsinducedsystemicresponse AT menghuage mesenchymalstemcelleducatedmacrophagesamelioratelpsinducedsystemicresponse AT lanfanghuang mesenchymalstemcelleducatedmacrophagesamelioratelpsinducedsystemicresponse AT linachen mesenchymalstemcelleducatedmacrophagesamelioratelpsinducedsystemicresponse AT baolicheng mesenchymalstemcelleducatedmacrophagesamelioratelpsinducedsystemicresponse AT qiangshu mesenchymalstemcelleducatedmacrophagesamelioratelpsinducedsystemicresponse AT jianguoxu mesenchymalstemcelleducatedmacrophagesamelioratelpsinducedsystemicresponse |