Diagnosis of SARS-CoV-2: Experience with rapid immunochromatography tests and RT‒qPCR
Background: Mexico has experienced six waves were experiences between 2019 and 2023 of coronavirus disease 2019 (COVID-19). In the first wave, diagnosis relied on RT‒qPCR; rapid tests for SARS‒CoV‒2 were introduced from the second wave onward. However, the patterns of results with this test in Mexic...
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| Language: | English |
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Elsevier
2025-06-01
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| Series: | Journal of Clinical Virology Plus |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2667038025000067 |
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| author | David Alejandro Cabrera Gaytán Porfirio Felipe Hernández Bautista Clara Esperanza Santacruz Tinoco Julio Elias Alvarado Yaah Yu-Mei Anguiano Hernández Bernardo Martínez Miguel Alfonso Vallejos Parás Lumumba Arriaga Nieto Nancy Sandoval Gutiérrez Concepción Grajales Muñiz Alejandro Moctezuma Paz Leticia Jaimes Betancourt |
| author_facet | David Alejandro Cabrera Gaytán Porfirio Felipe Hernández Bautista Clara Esperanza Santacruz Tinoco Julio Elias Alvarado Yaah Yu-Mei Anguiano Hernández Bernardo Martínez Miguel Alfonso Vallejos Parás Lumumba Arriaga Nieto Nancy Sandoval Gutiérrez Concepción Grajales Muñiz Alejandro Moctezuma Paz Leticia Jaimes Betancourt |
| author_sort | David Alejandro Cabrera Gaytán |
| collection | DOAJ |
| description | Background: Mexico has experienced six waves were experiences between 2019 and 2023 of coronavirus disease 2019 (COVID-19). In the first wave, diagnosis relied on RT‒qPCR; rapid tests for SARS‒CoV‒2 were introduced from the second wave onward. However, the patterns of results with this test in Mexico have not been documented in population-based studies. Objective: To describe the temporal patterns of the results from RT-qPCR and rapid immunochromatography tests for SARS-CoV-2 detection from the second to the sixth epidemic wave in Mexico. Materials and methods: This was a multicenter study at the national level. The operational definition of suspected cases of viral respiratory illness, confirmed cases of SARS-CoV-2 in Mexico, and data for asymptomatic individuals who required rapid testing between 2020 and 2023 were employed; testing was employed for the entirety of the four years. Positivity was estimated per epidemiological week, state, and condition for both tests. Spearman's correlation coefficient, trend chi-square analysis, and odds ratios with 95 % confidence intervals were used. Results: A total of 1749,765 cases had RT‒qPCR data recorded from 2020 to 2023, and 8356,903 cases with rapid tests were conducted for virus identification. Compared with the rest of the country, the southeastern region exhibited different patterns. The positivity rate of rapid tests from the epidemiological surveillance system was lower than that of RT‒qPCR during interepidemics periods, whereas the positivity rate of rapid tests for symptomatic individuals was higher than that of RT‒qPCR tests over two years. Conclusion: Rapid tests for SARS-CoV-2 identification in Mexico were affordable and timely at the local level. These tests revealed differing epidemic wave patterns in the southeastern region of the country. |
| format | Article |
| id | doaj-art-afcf047f724d468a89de9df03747abfb |
| institution | OA Journals |
| issn | 2667-0380 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Clinical Virology Plus |
| spelling | doaj-art-afcf047f724d468a89de9df03747abfb2025-08-20T02:23:11ZengElsevierJournal of Clinical Virology Plus2667-03802025-06-015210020710.1016/j.jcvp.2025.100207Diagnosis of SARS-CoV-2: Experience with rapid immunochromatography tests and RT‒qPCRDavid Alejandro Cabrera Gaytán0Porfirio Felipe Hernández Bautista1Clara Esperanza Santacruz Tinoco2Julio Elias Alvarado Yaah3Yu-Mei Anguiano Hernández4Bernardo Martínez Miguel5Alfonso Vallejos Parás6Lumumba Arriaga Nieto7Nancy Sandoval Gutiérrez8Concepción Grajales Muñiz9Alejandro Moctezuma Paz10Leticia Jaimes Betancourt11Coordinación de Calidad de Insumos y Laboratorios Especializados. Instituto Mexicano del Seguro Social, Ciudad de México, México; Corresponding author.Coordinación de Calidad de Insumos y Laboratorios Especializados. Instituto Mexicano del Seguro Social, Ciudad de México, MéxicoCoordinación de Calidad de Insumos y Laboratorios Especializados. Instituto Mexicano del Seguro Social, Ciudad de México, MéxicoCoordinación de Calidad de Insumos y Laboratorios Especializados. Instituto Mexicano del Seguro Social, Ciudad de México, MéxicoCoordinación de Calidad de Insumos y Laboratorios Especializados. Instituto Mexicano del Seguro Social, Ciudad de México, MéxicoCoordinación de Calidad de Insumos y Laboratorios Especializados. Instituto Mexicano del Seguro Social, Ciudad de México, MéxicoCoordinación de Vigilancia Epidemiológica, Instituto Mexicano del Seguro Social, Ciudad de México, MéxicoCoordinación de Vigilancia Epidemiológica, Instituto Mexicano del Seguro Social, Ciudad de México, MéxicoCoordinación de Calidad de Insumos y Laboratorios Especializados. Instituto Mexicano del Seguro Social, Ciudad de México, MéxicoOrganismo Público Descentralizado Servicios de Salud del Instituto Mexicano del Seguro Social para el Bienestar (IMSS-BIENESTAR), Ciudad de México, MéxicoCoordinación de Investigación en Salud, Instituto Mexicano del Seguro Social, Ciudad de México, MéxicoUnidad de Medicina Familiar No 7, Instituto Mexicano del Seguro Social, Ciudad de México, MéxicoBackground: Mexico has experienced six waves were experiences between 2019 and 2023 of coronavirus disease 2019 (COVID-19). In the first wave, diagnosis relied on RT‒qPCR; rapid tests for SARS‒CoV‒2 were introduced from the second wave onward. However, the patterns of results with this test in Mexico have not been documented in population-based studies. Objective: To describe the temporal patterns of the results from RT-qPCR and rapid immunochromatography tests for SARS-CoV-2 detection from the second to the sixth epidemic wave in Mexico. Materials and methods: This was a multicenter study at the national level. The operational definition of suspected cases of viral respiratory illness, confirmed cases of SARS-CoV-2 in Mexico, and data for asymptomatic individuals who required rapid testing between 2020 and 2023 were employed; testing was employed for the entirety of the four years. Positivity was estimated per epidemiological week, state, and condition for both tests. Spearman's correlation coefficient, trend chi-square analysis, and odds ratios with 95 % confidence intervals were used. Results: A total of 1749,765 cases had RT‒qPCR data recorded from 2020 to 2023, and 8356,903 cases with rapid tests were conducted for virus identification. Compared with the rest of the country, the southeastern region exhibited different patterns. The positivity rate of rapid tests from the epidemiological surveillance system was lower than that of RT‒qPCR during interepidemics periods, whereas the positivity rate of rapid tests for symptomatic individuals was higher than that of RT‒qPCR tests over two years. Conclusion: Rapid tests for SARS-CoV-2 identification in Mexico were affordable and timely at the local level. These tests revealed differing epidemic wave patterns in the southeastern region of the country.http://www.sciencedirect.com/science/article/pii/S2667038025000067RT‒qPCRSARS-CoV-2Rapid antigen testEpidemiological surveillanceRdRp gene |
| spellingShingle | David Alejandro Cabrera Gaytán Porfirio Felipe Hernández Bautista Clara Esperanza Santacruz Tinoco Julio Elias Alvarado Yaah Yu-Mei Anguiano Hernández Bernardo Martínez Miguel Alfonso Vallejos Parás Lumumba Arriaga Nieto Nancy Sandoval Gutiérrez Concepción Grajales Muñiz Alejandro Moctezuma Paz Leticia Jaimes Betancourt Diagnosis of SARS-CoV-2: Experience with rapid immunochromatography tests and RT‒qPCR Journal of Clinical Virology Plus RT‒qPCR SARS-CoV-2 Rapid antigen test Epidemiological surveillance RdRp gene |
| title | Diagnosis of SARS-CoV-2: Experience with rapid immunochromatography tests and RT‒qPCR |
| title_full | Diagnosis of SARS-CoV-2: Experience with rapid immunochromatography tests and RT‒qPCR |
| title_fullStr | Diagnosis of SARS-CoV-2: Experience with rapid immunochromatography tests and RT‒qPCR |
| title_full_unstemmed | Diagnosis of SARS-CoV-2: Experience with rapid immunochromatography tests and RT‒qPCR |
| title_short | Diagnosis of SARS-CoV-2: Experience with rapid immunochromatography tests and RT‒qPCR |
| title_sort | diagnosis of sars cov 2 experience with rapid immunochromatography tests and rt qpcr |
| topic | RT‒qPCR SARS-CoV-2 Rapid antigen test Epidemiological surveillance RdRp gene |
| url | http://www.sciencedirect.com/science/article/pii/S2667038025000067 |
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