Cxcr1 gene snp variability that affects mastitis resistance in holstein cows in türkiye
Genotyping 16 SNPs of C-X-C motif chemokine receptor 1 (CXCR1) gene region which affects host resistance against mastitis disease was carried out in Holstein cows raised in Türkiye. In this study, the frequency of the undesirable C allele in the CXCR1 c.771C>G polymorphic region, associated with...
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| Format: | Article |
| Language: | English |
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Kafkas University, Faculty of Veterinary Medicine
2024-12-01
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| Series: | Kafkas Universitesi Veteriner Fakültesi Dergisi |
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| Online Access: | https://vetdergikafkas.org/pdf.php?id=3169 |
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| author | Kozet AVANUS Alper YILMAZ Halil GÜNEŞ Ahmet ALTINEL Bülent EKİZ Hülya YALÇINTAN Dilara KEÇİCİ Nurşen DOĞAN |
| author_facet | Kozet AVANUS Alper YILMAZ Halil GÜNEŞ Ahmet ALTINEL Bülent EKİZ Hülya YALÇINTAN Dilara KEÇİCİ Nurşen DOĞAN |
| author_sort | Kozet AVANUS |
| collection | DOAJ |
| description | Genotyping 16 SNPs of C-X-C motif chemokine receptor 1 (CXCR1) gene region
which affects host resistance against mastitis disease was carried out in Holstein cows
raised in Türkiye. In this study, the frequency of the undesirable C allele in the CXCR1
c.771C>G polymorphic region, associated with an incomplete response, was found to be
high. Additionally, the genotypes c.1016AA and c.1016GG, which contribute to mastitis
resistance, were observed at low frequencies. Several SNP loci in the CXCR1 gene,
including c.606G>A, c.678G>A, c.1104G>A, c.1119+6C, c.1119+7A, and c.1119+10,
significantly deviated from Hardy-Weinberg equilibrium (HWE) (P<0.0001), indicating
violations of HWE assumptions such as random mating and absence of selection. The
deviations at c.606G>A, c.678G>A, and c.1104G>A suggest strong selection pressures,
likely due to artificial selection in Holstein cattle. These variants are synonymous
mutations that do not alter the amino acid sequence but may influence protein synthesis
through effects on mRNA stability, splicing, or translation efficiency. Furthermore,
the absence of heterozygotes at loci c.1119+6C, c.1119+7A, and c.1119+10, which
are located on untranslated regions (UTRs), potentially affecting gene expression by
regulating mRNA stability, localization, or translation initiation, points to genetic drift
or population substructure. These findings are important for understanding genetic
variability and can inform marker-assisted selection programs to enhance breeding
strategies while preserving genetic diversity for traits like disease resistance and milk
production. |
| format | Article |
| id | doaj-art-afc74e9603584e1884f74fe3c4e38562 |
| institution | Kabale University |
| issn | 1309-2251 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Kafkas University, Faculty of Veterinary Medicine |
| record_format | Article |
| series | Kafkas Universitesi Veteriner Fakültesi Dergisi |
| spelling | doaj-art-afc74e9603584e1884f74fe3c4e385622025-01-04T21:15:10ZengKafkas University, Faculty of Veterinary MedicineKafkas Universitesi Veteriner Fakültesi Dergisi1309-22512024-12-0131111912410.9775/kvfd.2024.329483169Cxcr1 gene snp variability that affects mastitis resistance in holstein cows in türkiyeKozet AVANUS0Alper YILMAZ1Halil GÜNEŞ2Ahmet ALTINEL3Bülent EKİZ4Hülya YALÇINTAN5Dilara KEÇİCİ6Nurşen DOĞAN7Istanbul University-Cerrahpasa, Faculty of Veterinary Medicine, Department of Animal Breeding & Husbandry, TR-34500 Istanbul - TÜRKİYEIstanbul University-Cerrahpasa, Faculty of Veterinary Medicine, Department of Animal Breeding & Husbandry, TR-34500 Istanbul - TÜRKİYEIstanbul University-Cerrahpasa, Faculty of Veterinary Medicine, Department of Animal Breeding & Husbandry, TR-34500 Istanbul - TÜRKİYEIstanbul Health and Technology University, Department of Biostatistics, TR-34445 Istanbul - TÜRKİYEIstanbul University-Cerrahpasa, Faculty of Veterinary Medicine, Department of Animal Breeding & Husbandry, TR-34500 Istanbul - TÜRKİYEIstanbul University-Cerrahpasa, Faculty of Veterinary Medicine, Department of Animal Breeding & Husbandry, TR-34500 Istanbul - TÜRKİYEIstanbul University-Cerrahpasa, Faculty of Veterinary Medicine, Department of Animal Breeding & Husbandry, TR-34500 Istanbul - TÜRKİYEIstanbul University-Cerrahpasa, Faculty of Veterinary Medicine, Department of Animal Breeding & Husbandry, TR-34500 Istanbul - TÜRKİYEGenotyping 16 SNPs of C-X-C motif chemokine receptor 1 (CXCR1) gene region which affects host resistance against mastitis disease was carried out in Holstein cows raised in Türkiye. In this study, the frequency of the undesirable C allele in the CXCR1 c.771C>G polymorphic region, associated with an incomplete response, was found to be high. Additionally, the genotypes c.1016AA and c.1016GG, which contribute to mastitis resistance, were observed at low frequencies. Several SNP loci in the CXCR1 gene, including c.606G>A, c.678G>A, c.1104G>A, c.1119+6C, c.1119+7A, and c.1119+10, significantly deviated from Hardy-Weinberg equilibrium (HWE) (P<0.0001), indicating violations of HWE assumptions such as random mating and absence of selection. The deviations at c.606G>A, c.678G>A, and c.1104G>A suggest strong selection pressures, likely due to artificial selection in Holstein cattle. These variants are synonymous mutations that do not alter the amino acid sequence but may influence protein synthesis through effects on mRNA stability, splicing, or translation efficiency. Furthermore, the absence of heterozygotes at loci c.1119+6C, c.1119+7A, and c.1119+10, which are located on untranslated regions (UTRs), potentially affecting gene expression by regulating mRNA stability, localization, or translation initiation, points to genetic drift or population substructure. These findings are important for understanding genetic variability and can inform marker-assisted selection programs to enhance breeding strategies while preserving genetic diversity for traits like disease resistance and milk production.https://vetdergikafkas.org/pdf.php?id=3169cattlepcrsnpmastitisresistance |
| spellingShingle | Kozet AVANUS Alper YILMAZ Halil GÜNEŞ Ahmet ALTINEL Bülent EKİZ Hülya YALÇINTAN Dilara KEÇİCİ Nurşen DOĞAN Cxcr1 gene snp variability that affects mastitis resistance in holstein cows in türkiye Kafkas Universitesi Veteriner Fakültesi Dergisi cattle pcr snp mastitis resistance |
| title | Cxcr1 gene snp variability that affects mastitis resistance in holstein cows in türkiye |
| title_full | Cxcr1 gene snp variability that affects mastitis resistance in holstein cows in türkiye |
| title_fullStr | Cxcr1 gene snp variability that affects mastitis resistance in holstein cows in türkiye |
| title_full_unstemmed | Cxcr1 gene snp variability that affects mastitis resistance in holstein cows in türkiye |
| title_short | Cxcr1 gene snp variability that affects mastitis resistance in holstein cows in türkiye |
| title_sort | cxcr1 gene snp variability that affects mastitis resistance in holstein cows in turkiye |
| topic | cattle pcr snp mastitis resistance |
| url | https://vetdergikafkas.org/pdf.php?id=3169 |
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