Microbiome of the Soil and Rhizosphere of the Halophyte <i>Spergularia marina</i> (L.) Griseb in the Saline Sites of Lake Kurgi, the South Urals: Metagenomic Analysis
The study of the metagenomes of bacterial communities in saline areas is relevant in connection with the global salinization of agricultural lands. The aim of this study was to investigate the biodiversity and structure of rhizobacterial communities associated with the halophyte <i>S. marina&l...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-03-01
|
| Series: | Microbiology Research |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2036-7481/16/3/64 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | The study of the metagenomes of bacterial communities in saline areas is relevant in connection with the global salinization of agricultural lands. The aim of this study was to investigate the biodiversity and structure of rhizobacterial communities associated with the halophyte <i>S. marina</i> from low and moderate sulfate–chloride salinity habitats. The bacterial community of bulk and rhizosphere soil was analyzed using high-throughput sequencing of the V1–V9 region of 16S rRNA by Oxford Nanopore Technologies. Alpha and beta diversity indices were calculated. A total of 55 phyla and 309 genera of bacteria were identified, among which <i>Proteobacteria</i> and <i>Bacteroidetes</i> dominated. The occurrence of <i>Planctomycetes</i>, <i>Verrucomicrobia</i>, and <i>Acidobacteria</i> in the rhizosphere was higher than in the bulk soil. Bacterial alpha diversity in the bulk soil decreased with increasing salinity, while it increased in the rhizosphere. The proportion of the halotolerant bacteria of <i>Flavobacterium</i> and <i>Alteromonas</i> genera significantly grew with increasing salinity both in the bulk and rhizosphere soil. In addition, in the rhizosphere, the percentage of <i>Comamonas</i>, <i>Methylibium</i>, <i>Lysobacter</i>, <i>Planctomyces</i>, <i>Sphingomonas</i>, <i>Stenotrophomonas</i>, and <i>Lewinella</i> genera increased. Among them, several genera included plant growth promoting rhizobacteria (PGPR). In the more saline bulk soil, the proportion of halotolerant genera <i>Bacillus</i>, <i>Salinimicrobium</i>, <i>Marinobacter</i>, <i>Clostridium</i>, <i>Euzebya</i>, <i>KSA1</i>, <i>Marinobacter, Clostridium</i>, <i>Salinimicrobium</i>, and <i>Halorhodospira</i> was also higher compared to the low saline site. Thus, increasing the salinity changed the taxonomic structure of the bacterial communities of both bulk soil and rhizosphere. |
|---|---|
| ISSN: | 2036-7481 |