Metagenomic and metabolomic analysis of gut microbiome's role in spinal cord injury recovery in rats
Spinal cord injury (SCI) induces profound systemic changes, including disruptions in gut microbiome composition and host metabolism. This study aimed to investigate the impact of SCI on gut microbial diversity and serum metabolites in rats, and to explore potential microbiome–metabolite interaction...
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Association of Basic Medical Sciences of Federation of Bosnia and Herzegovina
2025-03-01
|
| Series: | Biomolecules & Biomedicine |
| Subjects: | |
| Online Access: | http://www.bjbms.org/ojs/index.php/bjbms/article/view/12164 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849702002732302336 |
|---|---|
| author | Jieqi Zhang Xihan Ying Rong Hu Yi Huang Ruoqi Wang Lei Wu Dexiong Han Ruijie Ma Kelin He |
| author_facet | Jieqi Zhang Xihan Ying Rong Hu Yi Huang Ruoqi Wang Lei Wu Dexiong Han Ruijie Ma Kelin He |
| author_sort | Jieqi Zhang |
| collection | DOAJ |
| description |
Spinal cord injury (SCI) induces profound systemic changes, including disruptions in gut microbiome composition and host metabolism. This study aimed to investigate the impact of SCI on gut microbial diversity and serum metabolites in rats, and to explore potential microbiome–metabolite interactions that may influence recovery. Male Sprague–Dawley (SD) rats were assigned to either SCI or sham-operated groups. Fecal samples were collected for whole-genome metagenomic sequencing, and serum samples were analyzed using untargeted metabolomics. Gut microbial composition and diversity were assessed using α- and β-diversity indices, while Linear discriminant analysis effect size (LEfSe) identified differentially abundant taxa. Metabolomic pathway analysis was performed to detect significant changes in serum metabolites, and Spearman’s correlation was used to evaluate associations between gut microbes and metabolites. SCI significantly altered gut microbiota composition, with increased proportions of Ligilactobacillus and Staphylococcus, and decreased proportions of Lactobacillus and Limosilactobacillus. Metabolomic analysis revealed disrupted energy metabolism and elevated oxidative stress in SCI rats, as indicated by increased serum levels of pyruvate and lactic acid. Correlation analysis further identified significant associations between specific gut bacteria and key metabolites, suggesting microbiome-driven metabolic dysregulation following SCI. These findings highlight significant interactions between the gut microbiota and host metabolism after SCI and suggest that microbiome-targeted interventions may hold therapeutic potential for improving recovery by modulating metabolic function and oxidative stress responses.
|
| format | Article |
| id | doaj-art-afc7100a9e4b484da99edbc497692084 |
| institution | DOAJ |
| issn | 2831-0896 2831-090X |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Association of Basic Medical Sciences of Federation of Bosnia and Herzegovina |
| record_format | Article |
| series | Biomolecules & Biomedicine |
| spelling | doaj-art-afc7100a9e4b484da99edbc4976920842025-08-20T03:17:47ZengAssociation of Basic Medical Sciences of Federation of Bosnia and HerzegovinaBiomolecules & Biomedicine2831-08962831-090X2025-03-0110.17305/bb.2025.12164Metagenomic and metabolomic analysis of gut microbiome's role in spinal cord injury recovery in ratsJieqi Zhang0https://orcid.org/0009-0001-3392-3421Xihan Ying1https://orcid.org/0009-0009-8363-4394Rong Hu2Yi Huang3https://orcid.org/0009-0007-6359-9435Ruoqi Wang4https://orcid.org/0009-0006-2491-5039Lei Wu5Dexiong Han6Ruijie Ma7Kelin He8https://orcid.org/0000-0001-5563-6071Key Laboratory of Acupuncture and Neurology of Zhejiang Province, The Third School of Clinical Medicine (School of Rehabilitation Medicine), Zhejiang Chinese Medical University, Hangzhou, ChinaKey Laboratory of Acupuncture and Neurology of Zhejiang Province, The Third School of Clinical Medicine (School of Rehabilitation Medicine), Zhejiang Chinese Medical University, Hangzhou, ChinaKey Laboratory of Acupuncture and Neurology of Zhejiang Province, The Third School of Clinical Medicine (School of Rehabilitation Medicine), Zhejiang Chinese Medical University, Hangzhou, ChinaKey Laboratory of Acupuncture and Neurology of Zhejiang Province, The Third School of Clinical Medicine (School of Rehabilitation Medicine), Zhejiang Chinese Medical University, Hangzhou, ChinaKey Laboratory of Acupuncture and Neurology of Zhejiang Province, The Third School of Clinical Medicine (School of Rehabilitation Medicine), Zhejiang Chinese Medical University, Hangzhou, ChinaKey Laboratory of Acupuncture and Neurology of Zhejiang Province, The Third School of Clinical Medicine (School of Rehabilitation Medicine), Zhejiang Chinese Medical University, Hangzhou, China; Department of Acupuncture, The Third Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, ChinaKey Laboratory of Acupuncture and Neurology of Zhejiang Province, The Third School of Clinical Medicine (School of Rehabilitation Medicine), Zhejiang Chinese Medical University, Hangzhou, China; Department of Acupuncture, The Third Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, ChinaKey Laboratory of Acupuncture and Neurology of Zhejiang Province, The Third School of Clinical Medicine (School of Rehabilitation Medicine), Zhejiang Chinese Medical University, Hangzhou, China; Department of Acupuncture, The Third Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, ChinaKey Laboratory of Acupuncture and Neurology of Zhejiang Province, The Third School of Clinical Medicine (School of Rehabilitation Medicine), Zhejiang Chinese Medical University, Hangzhou, China; Department of Acupuncture, The Third Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, China Spinal cord injury (SCI) induces profound systemic changes, including disruptions in gut microbiome composition and host metabolism. This study aimed to investigate the impact of SCI on gut microbial diversity and serum metabolites in rats, and to explore potential microbiome–metabolite interactions that may influence recovery. Male Sprague–Dawley (SD) rats were assigned to either SCI or sham-operated groups. Fecal samples were collected for whole-genome metagenomic sequencing, and serum samples were analyzed using untargeted metabolomics. Gut microbial composition and diversity were assessed using α- and β-diversity indices, while Linear discriminant analysis effect size (LEfSe) identified differentially abundant taxa. Metabolomic pathway analysis was performed to detect significant changes in serum metabolites, and Spearman’s correlation was used to evaluate associations between gut microbes and metabolites. SCI significantly altered gut microbiota composition, with increased proportions of Ligilactobacillus and Staphylococcus, and decreased proportions of Lactobacillus and Limosilactobacillus. Metabolomic analysis revealed disrupted energy metabolism and elevated oxidative stress in SCI rats, as indicated by increased serum levels of pyruvate and lactic acid. Correlation analysis further identified significant associations between specific gut bacteria and key metabolites, suggesting microbiome-driven metabolic dysregulation following SCI. These findings highlight significant interactions between the gut microbiota and host metabolism after SCI and suggest that microbiome-targeted interventions may hold therapeutic potential for improving recovery by modulating metabolic function and oxidative stress responses. http://www.bjbms.org/ojs/index.php/bjbms/article/view/12164Spinal cord injurySCImetagenomicsmetabolomicsgut microbiome |
| spellingShingle | Jieqi Zhang Xihan Ying Rong Hu Yi Huang Ruoqi Wang Lei Wu Dexiong Han Ruijie Ma Kelin He Metagenomic and metabolomic analysis of gut microbiome's role in spinal cord injury recovery in rats Biomolecules & Biomedicine Spinal cord injury SCI metagenomics metabolomics gut microbiome |
| title | Metagenomic and metabolomic analysis of gut microbiome's role in spinal cord injury recovery in rats |
| title_full | Metagenomic and metabolomic analysis of gut microbiome's role in spinal cord injury recovery in rats |
| title_fullStr | Metagenomic and metabolomic analysis of gut microbiome's role in spinal cord injury recovery in rats |
| title_full_unstemmed | Metagenomic and metabolomic analysis of gut microbiome's role in spinal cord injury recovery in rats |
| title_short | Metagenomic and metabolomic analysis of gut microbiome's role in spinal cord injury recovery in rats |
| title_sort | metagenomic and metabolomic analysis of gut microbiome s role in spinal cord injury recovery in rats |
| topic | Spinal cord injury SCI metagenomics metabolomics gut microbiome |
| url | http://www.bjbms.org/ojs/index.php/bjbms/article/view/12164 |
| work_keys_str_mv | AT jieqizhang metagenomicandmetabolomicanalysisofgutmicrobiomesroleinspinalcordinjuryrecoveryinrats AT xihanying metagenomicandmetabolomicanalysisofgutmicrobiomesroleinspinalcordinjuryrecoveryinrats AT ronghu metagenomicandmetabolomicanalysisofgutmicrobiomesroleinspinalcordinjuryrecoveryinrats AT yihuang metagenomicandmetabolomicanalysisofgutmicrobiomesroleinspinalcordinjuryrecoveryinrats AT ruoqiwang metagenomicandmetabolomicanalysisofgutmicrobiomesroleinspinalcordinjuryrecoveryinrats AT leiwu metagenomicandmetabolomicanalysisofgutmicrobiomesroleinspinalcordinjuryrecoveryinrats AT dexionghan metagenomicandmetabolomicanalysisofgutmicrobiomesroleinspinalcordinjuryrecoveryinrats AT ruijiema metagenomicandmetabolomicanalysisofgutmicrobiomesroleinspinalcordinjuryrecoveryinrats AT kelinhe metagenomicandmetabolomicanalysisofgutmicrobiomesroleinspinalcordinjuryrecoveryinrats |