Study of the immunotoxic properties of pegylated hyaluronidase
The development of safe drugs occupies a special place in the pharmaceutical industry. One of the main tasks of its preclinical phase is to evaluate possible toxic effects of the developed drug on the body and on various systems, including the immune system. The aim of our work was to study immunoto...
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Russian Academy of Sciences, Siberian Branch Publishing House
2023-10-01
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| Series: | Сибирский научный медицинский журнал |
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| Online Access: | https://sibmed.elpub.ru/jour/article/view/1249 |
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| author | K. I. Ershov A. M. Shvetsova E. Yu. Sherstoboev A. A. Churin V. V. Zhdanov M. A. Korolev P. G. Madonov |
| author_facet | K. I. Ershov A. M. Shvetsova E. Yu. Sherstoboev A. A. Churin V. V. Zhdanov M. A. Korolev P. G. Madonov |
| author_sort | K. I. Ershov |
| collection | DOAJ |
| description | The development of safe drugs occupies a special place in the pharmaceutical industry. One of the main tasks of its preclinical phase is to evaluate possible toxic effects of the developed drug on the body and on various systems, including the immune system. The aim of our work was to study immunotoxic properties of pegylated hyaluronidase (PEG- HYAL). Material and methods. Mice F1(CBA/C57Bl/6) were divided into subgroups which were intragastric and intraperitoneally administered with PEG-HYAL in different dosages (50, 500, 1250, 2500 and 5000 U/kg). The number of antibody-producing cells in the spleen, the mass and cellularity of central and peripheral immune organs, phagocytic activity of peritoneal macrophages and neutrophils, delayed hypersensitivity reaction (DHR), level of hemagglutinin to sheep erythrocytes, spontaneous and mitogen-induced splenocyte proliferation were determined. Results. PEG-HYAL did not induce DHR, did not suppress phagocyte activity of peritoneal macrophages, at a dose of 50 ED/kg did not significantly affect the hemagglutinin content to erythrocytes, but at a dose of 500 ED/kg did statistically significantly reduce the titer of specific antibodies. When experimental animals were exposed to PEG-HYAL at doses of 50 and 500 U/kg, spontaneous and mitogen-induced proliferation of splenocytes decreased. Conclusions. The PEG-HYAL trial produced results that can be used to substantiate the administration of the PEG-HYAL-based medication. |
| format | Article |
| id | doaj-art-afc4df813e9042029a6cdddd535ea33a |
| institution | Kabale University |
| issn | 2410-2512 2410-2520 |
| language | Russian |
| publishDate | 2023-10-01 |
| publisher | Russian Academy of Sciences, Siberian Branch Publishing House |
| record_format | Article |
| series | Сибирский научный медицинский журнал |
| spelling | doaj-art-afc4df813e9042029a6cdddd535ea33a2025-08-20T03:50:44ZrusRussian Academy of Sciences, Siberian Branch Publishing HouseСибирский научный медицинский журнал2410-25122410-25202023-10-0143511812610.18699/SSMJ20230512545Study of the immunotoxic properties of pegylated hyaluronidaseK. I. Ershov0A. M. Shvetsova1E. Yu. Sherstoboev2A. A. Churin3V. V. Zhdanov4M. A. Korolev5P. G. Madonov6Novosibirsk State Medical University of Minzdrav of Russia; Research Institute of Clinical and Experimental Lymphology – Branch of the Federal Research Center Institute of Cytology and Genetics SB RASResearch Institute of Clinical and Experimental Lymphology – Branch of the Federal Research Center Institute of Cytology and Genetics SB RASResearch Institute of Pharmacology and Regenerative Medicine E.D. Goldberg Tomsk National Research Medical Center of the Russian Academy of SciencesResearch Institute of Pharmacology and Regenerative Medicine E.D. Goldberg Tomsk National Research Medical Center of the Russian Academy of SciencesResearch Institute of Pharmacology and Regenerative Medicine E.D. Goldberg Tomsk National Research Medical Center of the Russian Academy of SciencesResearch Institute of Clinical and Experimental Lymphology – Branch of the Federal Research Center Institute of Cytology and Genetics SB RASNovosibirsk State Medical University of Minzdrav of Russia; Research Institute of Clinical and Experimental Lymphology – Branch of the Federal Research Center Institute of Cytology and Genetics SB RASThe development of safe drugs occupies a special place in the pharmaceutical industry. One of the main tasks of its preclinical phase is to evaluate possible toxic effects of the developed drug on the body and on various systems, including the immune system. The aim of our work was to study immunotoxic properties of pegylated hyaluronidase (PEG- HYAL). Material and methods. Mice F1(CBA/C57Bl/6) were divided into subgroups which were intragastric and intraperitoneally administered with PEG-HYAL in different dosages (50, 500, 1250, 2500 and 5000 U/kg). The number of antibody-producing cells in the spleen, the mass and cellularity of central and peripheral immune organs, phagocytic activity of peritoneal macrophages and neutrophils, delayed hypersensitivity reaction (DHR), level of hemagglutinin to sheep erythrocytes, spontaneous and mitogen-induced splenocyte proliferation were determined. Results. PEG-HYAL did not induce DHR, did not suppress phagocyte activity of peritoneal macrophages, at a dose of 50 ED/kg did not significantly affect the hemagglutinin content to erythrocytes, but at a dose of 500 ED/kg did statistically significantly reduce the titer of specific antibodies. When experimental animals were exposed to PEG-HYAL at doses of 50 and 500 U/kg, spontaneous and mitogen-induced proliferation of splenocytes decreased. Conclusions. The PEG-HYAL trial produced results that can be used to substantiate the administration of the PEG-HYAL-based medication.https://sibmed.elpub.ru/jour/article/view/1249pegylated hyaluronidaseimmunotoxic properties |
| spellingShingle | K. I. Ershov A. M. Shvetsova E. Yu. Sherstoboev A. A. Churin V. V. Zhdanov M. A. Korolev P. G. Madonov Study of the immunotoxic properties of pegylated hyaluronidase Сибирский научный медицинский журнал pegylated hyaluronidase immunotoxic properties |
| title | Study of the immunotoxic properties of pegylated hyaluronidase |
| title_full | Study of the immunotoxic properties of pegylated hyaluronidase |
| title_fullStr | Study of the immunotoxic properties of pegylated hyaluronidase |
| title_full_unstemmed | Study of the immunotoxic properties of pegylated hyaluronidase |
| title_short | Study of the immunotoxic properties of pegylated hyaluronidase |
| title_sort | study of the immunotoxic properties of pegylated hyaluronidase |
| topic | pegylated hyaluronidase immunotoxic properties |
| url | https://sibmed.elpub.ru/jour/article/view/1249 |
| work_keys_str_mv | AT kiershov studyoftheimmunotoxicpropertiesofpegylatedhyaluronidase AT amshvetsova studyoftheimmunotoxicpropertiesofpegylatedhyaluronidase AT eyusherstoboev studyoftheimmunotoxicpropertiesofpegylatedhyaluronidase AT aachurin studyoftheimmunotoxicpropertiesofpegylatedhyaluronidase AT vvzhdanov studyoftheimmunotoxicpropertiesofpegylatedhyaluronidase AT makorolev studyoftheimmunotoxicpropertiesofpegylatedhyaluronidase AT pgmadonov studyoftheimmunotoxicpropertiesofpegylatedhyaluronidase |