TRIM3 suppressed the tumorigenicity of melanoma cells by ubiquitinating YAP1
Abstract Tripartite motif-containing 3 (TRIM 3), as a vital member of TRIM family, has been receiving significant attention in cancer research. This research aims to detect the effect and relevant molecular functions of TRIM3 in melanoma cells.GEPIA website and immunohistochemical analysis were perf...
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Nature Portfolio
2025-07-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-025-11317-y |
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| author | Yi-tong Wang Jing-chen Zhao Ding-ming Song Jun-jiang Chen |
| author_facet | Yi-tong Wang Jing-chen Zhao Ding-ming Song Jun-jiang Chen |
| author_sort | Yi-tong Wang |
| collection | DOAJ |
| description | Abstract Tripartite motif-containing 3 (TRIM 3), as a vital member of TRIM family, has been receiving significant attention in cancer research. This research aims to detect the effect and relevant molecular functions of TRIM3 in melanoma cells.GEPIA website and immunohistochemical analysis were performed to investigate TRIM3 level in melanoma samples. The growth ability, the mobility, and the apoptosis of M14 and A375 cells were determined by biological experiments. qPCR and western blot assays were performed to evaluate the expression of key genes. The interaction between TRIM3 and YAP1 was verified by immunoprecipitation and ubiquitination assays.A reduction of TRIM3 was observed in melanoma samples, and the loss of TRIM3 strengthened the proliferation and mobility of M14 and A375 cells and diminished apoptosis, and vice versa. TRIM3 expression only affected the protein level of YAP1, while YAP1 mRNA was not changed. Then, we demonstrated that TRIM3 directly interacted with YAP1, and TRIM3 reduced YAP1 stability by inducing ubiquitination modification. Finally, rescue assays showed that si-YAP1 treatment alleviated the effects of si-TRIM3 on melanoma cells.This study indicated that depletion of TRIM3 strengthened the proliferation and mobility of M14 and A375 cells, suppressed cell apoptosis, but these phenomena were counteracted by YAP1 down-regulation. |
| format | Article |
| id | doaj-art-afb9e9542667467cb771b3fad32e2228 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-afb9e9542667467cb771b3fad32e22282025-08-20T04:01:51ZengNature PortfolioScientific Reports2045-23222025-07-0115111010.1038/s41598-025-11317-yTRIM3 suppressed the tumorigenicity of melanoma cells by ubiquitinating YAP1Yi-tong Wang0Jing-chen Zhao1Ding-ming Song2Jun-jiang Chen3Department of Burns and Plastic Surgery, The First Affiliated Hospital of Jinzhou Medical UniversityDepartment of Burns and Plastic Surgery, The First Affiliated Hospital of Jinzhou Medical UniversityDepartment of Urology, The First Affiliated Hospital of Jinzhou Medical UniversityDepartment of Burns and Plastic Surgery, The First Affiliated Hospital of Jinzhou Medical UniversityAbstract Tripartite motif-containing 3 (TRIM 3), as a vital member of TRIM family, has been receiving significant attention in cancer research. This research aims to detect the effect and relevant molecular functions of TRIM3 in melanoma cells.GEPIA website and immunohistochemical analysis were performed to investigate TRIM3 level in melanoma samples. The growth ability, the mobility, and the apoptosis of M14 and A375 cells were determined by biological experiments. qPCR and western blot assays were performed to evaluate the expression of key genes. The interaction between TRIM3 and YAP1 was verified by immunoprecipitation and ubiquitination assays.A reduction of TRIM3 was observed in melanoma samples, and the loss of TRIM3 strengthened the proliferation and mobility of M14 and A375 cells and diminished apoptosis, and vice versa. TRIM3 expression only affected the protein level of YAP1, while YAP1 mRNA was not changed. Then, we demonstrated that TRIM3 directly interacted with YAP1, and TRIM3 reduced YAP1 stability by inducing ubiquitination modification. Finally, rescue assays showed that si-YAP1 treatment alleviated the effects of si-TRIM3 on melanoma cells.This study indicated that depletion of TRIM3 strengthened the proliferation and mobility of M14 and A375 cells, suppressed cell apoptosis, but these phenomena were counteracted by YAP1 down-regulation.https://doi.org/10.1038/s41598-025-11317-yMelanomaTRIM3UbiquitinationYAP1 |
| spellingShingle | Yi-tong Wang Jing-chen Zhao Ding-ming Song Jun-jiang Chen TRIM3 suppressed the tumorigenicity of melanoma cells by ubiquitinating YAP1 Scientific Reports Melanoma TRIM3 Ubiquitination YAP1 |
| title | TRIM3 suppressed the tumorigenicity of melanoma cells by ubiquitinating YAP1 |
| title_full | TRIM3 suppressed the tumorigenicity of melanoma cells by ubiquitinating YAP1 |
| title_fullStr | TRIM3 suppressed the tumorigenicity of melanoma cells by ubiquitinating YAP1 |
| title_full_unstemmed | TRIM3 suppressed the tumorigenicity of melanoma cells by ubiquitinating YAP1 |
| title_short | TRIM3 suppressed the tumorigenicity of melanoma cells by ubiquitinating YAP1 |
| title_sort | trim3 suppressed the tumorigenicity of melanoma cells by ubiquitinating yap1 |
| topic | Melanoma TRIM3 Ubiquitination YAP1 |
| url | https://doi.org/10.1038/s41598-025-11317-y |
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