Assessing the prevalence of human enteric viruses in hospital wastewater to evaluate the effectiveness of wastewater treatment systems
In this experiment, we employed Real-time PCR(RT-PCR) and metagenomic Next-Generation Sequencing (mNGS) techniques to detect the presence of Norovirus, Rotavirus Group A, Adenovirus Group F, and Astrovirus in untreated sewage from three major hospitals. A comparison with clinical lab test outcomes r...
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| Language: | English |
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Elsevier
2025-01-01
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| Series: | Ecotoxicology and Environmental Safety |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651324015641 |
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| author | Xuebing Zhou Qingcao Li Zhanying Shi Wenbo Lu Chunhui Shu Junyao Zhu Yong Wu |
| author_facet | Xuebing Zhou Qingcao Li Zhanying Shi Wenbo Lu Chunhui Shu Junyao Zhu Yong Wu |
| author_sort | Xuebing Zhou |
| collection | DOAJ |
| description | In this experiment, we employed Real-time PCR(RT-PCR) and metagenomic Next-Generation Sequencing (mNGS) techniques to detect the presence of Norovirus, Rotavirus Group A, Adenovirus Group F, and Astrovirus in untreated sewage from three major hospitals. A comparison with clinical lab test outcomes revealed Norovirus as having the highest infection rate, followed by Adenovirus Group F and Rotavirus Group A. Despite not testing for Astrovirus in clinical labs, its sewage detection rate was surpassed only by Norovirus, suggesting a potentially high clinical infection rate. Further analysis of these viruses in treated sewage revealed that chlorination failed to eliminate the virus, maintaining viral concentrations in the treated sewage between 10^2 and 10^3 copies/ml. Even though nucleic acid testing methods fail to detect viral actions, the possible danger they present to public safety should not be ignored. During this experiment, viral nucleic acid was extracted directly from the samples without prior concentration. This method, unlike conventional virus detection post-concentration, bypasses concerns such as recovery efficiency, offering a clearer representation of virus concentrations in water samples and facilitating easier operation. |
| format | Article |
| id | doaj-art-afb5b415a7a84e078ac884ac9a6a5b4c |
| institution | Kabale University |
| issn | 0147-6513 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj-art-afb5b415a7a84e078ac884ac9a6a5b4c2025-01-23T05:25:45ZengElsevierEcotoxicology and Environmental Safety0147-65132025-01-01289117488Assessing the prevalence of human enteric viruses in hospital wastewater to evaluate the effectiveness of wastewater treatment systemsXuebing Zhou0Qingcao Li1Zhanying Shi2Wenbo Lu3Chunhui Shu4Junyao Zhu5Yong Wu6Department of Clinical Laboratory, No.906 Hospital of People's Liberation Army, Ningbo, ChinaDepartment of Clinical Laboratory, The Affiliated LiHuiLi Hospital of Ningbo University, Ningbo, China; Corresponding author.Department of Clinical Laboratory, No.906 Hospital of People's Liberation Army, Ningbo, ChinaDepartment of Clinical Laboratory, Ningbo Women and Children's Hospital, Ningbo, ChinaDepartment of Laboratory Medicine, Ningbo Mingzhou Hospital, Ningbo, ChinaDepartment of Clinical Laboratory, No.906 Hospital of People's Liberation Army, Ningbo, ChinaDepartment of Clinical Laboratory, No.906 Hospital of People's Liberation Army, Ningbo, ChinaIn this experiment, we employed Real-time PCR(RT-PCR) and metagenomic Next-Generation Sequencing (mNGS) techniques to detect the presence of Norovirus, Rotavirus Group A, Adenovirus Group F, and Astrovirus in untreated sewage from three major hospitals. A comparison with clinical lab test outcomes revealed Norovirus as having the highest infection rate, followed by Adenovirus Group F and Rotavirus Group A. Despite not testing for Astrovirus in clinical labs, its sewage detection rate was surpassed only by Norovirus, suggesting a potentially high clinical infection rate. Further analysis of these viruses in treated sewage revealed that chlorination failed to eliminate the virus, maintaining viral concentrations in the treated sewage between 10^2 and 10^3 copies/ml. Even though nucleic acid testing methods fail to detect viral actions, the possible danger they present to public safety should not be ignored. During this experiment, viral nucleic acid was extracted directly from the samples without prior concentration. This method, unlike conventional virus detection post-concentration, bypasses concerns such as recovery efficiency, offering a clearer representation of virus concentrations in water samples and facilitating easier operation.http://www.sciencedirect.com/science/article/pii/S0147651324015641Hospital wastewaterWastewater-based epidemiology (WBE)Real-time PCR (RT-PCR)Metagenomic Next-Generation Sequencing (mNGS)Enteric virusesWastewater treatment |
| spellingShingle | Xuebing Zhou Qingcao Li Zhanying Shi Wenbo Lu Chunhui Shu Junyao Zhu Yong Wu Assessing the prevalence of human enteric viruses in hospital wastewater to evaluate the effectiveness of wastewater treatment systems Ecotoxicology and Environmental Safety Hospital wastewater Wastewater-based epidemiology (WBE) Real-time PCR (RT-PCR) Metagenomic Next-Generation Sequencing (mNGS) Enteric viruses Wastewater treatment |
| title | Assessing the prevalence of human enteric viruses in hospital wastewater to evaluate the effectiveness of wastewater treatment systems |
| title_full | Assessing the prevalence of human enteric viruses in hospital wastewater to evaluate the effectiveness of wastewater treatment systems |
| title_fullStr | Assessing the prevalence of human enteric viruses in hospital wastewater to evaluate the effectiveness of wastewater treatment systems |
| title_full_unstemmed | Assessing the prevalence of human enteric viruses in hospital wastewater to evaluate the effectiveness of wastewater treatment systems |
| title_short | Assessing the prevalence of human enteric viruses in hospital wastewater to evaluate the effectiveness of wastewater treatment systems |
| title_sort | assessing the prevalence of human enteric viruses in hospital wastewater to evaluate the effectiveness of wastewater treatment systems |
| topic | Hospital wastewater Wastewater-based epidemiology (WBE) Real-time PCR (RT-PCR) Metagenomic Next-Generation Sequencing (mNGS) Enteric viruses Wastewater treatment |
| url | http://www.sciencedirect.com/science/article/pii/S0147651324015641 |
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