Nonlinear Magnetic Response Measurements in Study of Magnetic Nanoparticles Uptake by Mesenchymal Stem Cells
Stem cells therapies offer a promising approach in translational oncology, as well as in regenerative medicine due to the tropism of these cells to the damage site. To track the distribution of stem cells, the latter could be labeled by MRI-sensitive superparamagnetic (SPM) iron oxide nanoparticles....
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MDPI AG
2025-04-01
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| author | Vyacheslav Ryzhov Yaroslav Marchenko Vladimir Deriglazov Natalia Yudintceva Oleg Smirnov Alexandr Arutyunyan Tatiana Shtam Evgenii Ivanov Stephanie E. Combs Maxim Shevtsov |
| author_facet | Vyacheslav Ryzhov Yaroslav Marchenko Vladimir Deriglazov Natalia Yudintceva Oleg Smirnov Alexandr Arutyunyan Tatiana Shtam Evgenii Ivanov Stephanie E. Combs Maxim Shevtsov |
| author_sort | Vyacheslav Ryzhov |
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| description | Stem cells therapies offer a promising approach in translational oncology, as well as in regenerative medicine due to the tropism of these cells to the damage site. To track the distribution of stem cells, the latter could be labeled by MRI-sensitive superparamagnetic (SPM) iron oxide nanoparticles. In the current study, magnetic properties of the magnetic nanoparticles (MNPs) incorporated into the bone marrow-derived fetal mesenchymal stem cells (FetMSCs) were evaluated employing nonlinear magnetic response measurements. Synthesized dextran-coated iron oxide nanoparticles were additionally characterized by X-ray diffraction, transmission electron microscopy, and dynamic light scattering. The MNP uptake by the FetMSCs 24 h following coincubation was studied by longitudinal nonlinear response to weak alternating magnetic field with registration of the second harmonic of magnetization. Subsequent data processing using a formalism based on the numerical solution of the Fokker–Planck kinetic equation allowed us to determine magnetic and dynamic parameters and the state of MNPs in the cells, as well as in the culture medium. It was found that MNPs formed aggregates in the culture medium; they were absorbed by the cells during coincubation. The aggregates exhibited SPM regime in the medium, and the parameters of the MNP aggregates remained virtually unchanged in the cells, indicating the preservation of the aggregation state of MNPs inside the cells. This implies also the preservation of the organic shell of the nanoparticles inside FetMSCs. The accumulation of MNPs by mesenchymal stem cells gradually increased with the concentration of MNPs. Thus, the study confirmed that the labeling of MSCs with MNPs is an effective method for subsequent cell tracking as incorporated nanoparticles retain their magnetic properties. |
| format | Article |
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| language | English |
| publishDate | 2025-04-01 |
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| series | Nanomaterials |
| spelling | doaj-art-afb576afbffe41f6aad99376c3d15b2d2025-08-20T02:31:08ZengMDPI AGNanomaterials2079-49912025-04-0115967510.3390/nano15090675Nonlinear Magnetic Response Measurements in Study of Magnetic Nanoparticles Uptake by Mesenchymal Stem CellsVyacheslav Ryzhov0Yaroslav Marchenko1Vladimir Deriglazov2Natalia Yudintceva3Oleg Smirnov4Alexandr Arutyunyan5Tatiana Shtam6Evgenii Ivanov7Stephanie E. Combs8Maxim Shevtsov9Petersburg Nuclear Physics Institute Named by B.P. Konstantinov of National Research Centre «Kurchatov Institute», Orlova Roscha 1, 188300 Gatchina, RussiaPetersburg Nuclear Physics Institute Named by B.P. Konstantinov of National Research Centre «Kurchatov Institute», Orlova Roscha 1, 188300 Gatchina, RussiaPetersburg Nuclear Physics Institute Named by B.P. Konstantinov of National Research Centre «Kurchatov Institute», Orlova Roscha 1, 188300 Gatchina, RussiaLaboratory of Biomedical Nanotechnologies, Institute of Cytology of the Russian Academy of Sciences (RAS), 194064 St. Petersburg, RussiaPetersburg Nuclear Physics Institute Named by B.P. Konstantinov of National Research Centre «Kurchatov Institute», Orlova Roscha 1, 188300 Gatchina, RussiaPetersburg Nuclear Physics Institute Named by B.P. Konstantinov of National Research Centre «Kurchatov Institute», Orlova Roscha 1, 188300 Gatchina, RussiaPetersburg Nuclear Physics Institute Named by B.P. Konstantinov of National Research Centre «Kurchatov Institute», Orlova Roscha 1, 188300 Gatchina, RussiaLLC “SPF “HELIX”, Sampsonievsky B. Prospect, 20 Litera A, 194044 St. Petersburg, RussiaDepartment of Radiation Oncology, Klinikum Rechts der Isar, Technical University of Munich, Ismaninger Str. 22, 81675 Munich, GermanyLaboratory of Biomedical Nanotechnologies, Institute of Cytology of the Russian Academy of Sciences (RAS), 194064 St. Petersburg, RussiaStem cells therapies offer a promising approach in translational oncology, as well as in regenerative medicine due to the tropism of these cells to the damage site. To track the distribution of stem cells, the latter could be labeled by MRI-sensitive superparamagnetic (SPM) iron oxide nanoparticles. In the current study, magnetic properties of the magnetic nanoparticles (MNPs) incorporated into the bone marrow-derived fetal mesenchymal stem cells (FetMSCs) were evaluated employing nonlinear magnetic response measurements. Synthesized dextran-coated iron oxide nanoparticles were additionally characterized by X-ray diffraction, transmission electron microscopy, and dynamic light scattering. The MNP uptake by the FetMSCs 24 h following coincubation was studied by longitudinal nonlinear response to weak alternating magnetic field with registration of the second harmonic of magnetization. Subsequent data processing using a formalism based on the numerical solution of the Fokker–Planck kinetic equation allowed us to determine magnetic and dynamic parameters and the state of MNPs in the cells, as well as in the culture medium. It was found that MNPs formed aggregates in the culture medium; they were absorbed by the cells during coincubation. The aggregates exhibited SPM regime in the medium, and the parameters of the MNP aggregates remained virtually unchanged in the cells, indicating the preservation of the aggregation state of MNPs inside the cells. This implies also the preservation of the organic shell of the nanoparticles inside FetMSCs. The accumulation of MNPs by mesenchymal stem cells gradually increased with the concentration of MNPs. Thus, the study confirmed that the labeling of MSCs with MNPs is an effective method for subsequent cell tracking as incorporated nanoparticles retain their magnetic properties.https://www.mdpi.com/2079-4991/15/9/675magnetic nanoparticlesmesenchymal stem cellslongitudinal response to weak alternating (ac) magnetic field |
| spellingShingle | Vyacheslav Ryzhov Yaroslav Marchenko Vladimir Deriglazov Natalia Yudintceva Oleg Smirnov Alexandr Arutyunyan Tatiana Shtam Evgenii Ivanov Stephanie E. Combs Maxim Shevtsov Nonlinear Magnetic Response Measurements in Study of Magnetic Nanoparticles Uptake by Mesenchymal Stem Cells Nanomaterials magnetic nanoparticles mesenchymal stem cells longitudinal response to weak alternating (ac) magnetic field |
| title | Nonlinear Magnetic Response Measurements in Study of Magnetic Nanoparticles Uptake by Mesenchymal Stem Cells |
| title_full | Nonlinear Magnetic Response Measurements in Study of Magnetic Nanoparticles Uptake by Mesenchymal Stem Cells |
| title_fullStr | Nonlinear Magnetic Response Measurements in Study of Magnetic Nanoparticles Uptake by Mesenchymal Stem Cells |
| title_full_unstemmed | Nonlinear Magnetic Response Measurements in Study of Magnetic Nanoparticles Uptake by Mesenchymal Stem Cells |
| title_short | Nonlinear Magnetic Response Measurements in Study of Magnetic Nanoparticles Uptake by Mesenchymal Stem Cells |
| title_sort | nonlinear magnetic response measurements in study of magnetic nanoparticles uptake by mesenchymal stem cells |
| topic | magnetic nanoparticles mesenchymal stem cells longitudinal response to weak alternating (ac) magnetic field |
| url | https://www.mdpi.com/2079-4991/15/9/675 |
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