The Impact of Silver Nanoparticles on Dentinal Tubule Penetration of Endodontic Bioceramic Sealer
The impact of adding silver nanoparticles (AgNPs) to bioceramic (BC) sealer on their ability to penetrate dentinal tubules is still unknown. Thus, this confocal laser scanning microscopic (CLSM) study aimed to assess the extent of dentinal tubule penetration of BC sealer (TotalFill<sup>®</s...
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2024-12-01
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| author | Sundus Bukhary Sarah Alkahtany Amal Almohaimede Nourah Alkhayatt Shahad Alsulaiman Salma Alohali |
| author_facet | Sundus Bukhary Sarah Alkahtany Amal Almohaimede Nourah Alkhayatt Shahad Alsulaiman Salma Alohali |
| author_sort | Sundus Bukhary |
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| description | The impact of adding silver nanoparticles (AgNPs) to bioceramic (BC) sealer on their ability to penetrate dentinal tubules is still unknown. Thus, this confocal laser scanning microscopic (CLSM) study aimed to assess the extent of dentinal tubule penetration of BC sealer (TotalFill<sup>®</sup> Hiflow BC Sealer™, FKG, Switzerland) with and without AgNPs using the single-cone (SC) technique and the continuous-wave condensation (CWC) technique. AgNPs alone as well as in a mixture with the BC sealer were characterized using scanning electron microscopy and transmission electron microscopy. Single-rooted extracted human teeth (N = 100) were selected and prepared, and then divided into four groups (n = 25). Group 1 (BC/SC): BC sealer obturated with the SC technique. Group 2 (BC+AgNPs/SC): BC sealer with AgNPs obturated with the SC technique. Group 3 (BC/CWC): BC Sealer obturated with the CWC technique. Group 4 (BC+AgNPs/CWC): BC Sealer with AgNPs obturated with the CWC technique. After 2 weeks, roots were horizontally sectioned to obtain 1 mm thick dentin slices that were evaluated with CLSM. Sealer dentinal tubule penetration area and the maximum depth of penetration were measured. Data were analyzed with one-way ANOVA and the Tukey multiple comparison tests (<i>p</i> ≤ 0.05). The characterization process demonstrated a spherical-shaped nanoparticles without obvious agglomeration. The results showed that Group 2 (BC+AgNPs/SC) significantly demonstrated the highest mean tubular penetration depth, while group 3 (BC/CWC) had the lowest mean depth. Group 2 (BC+AgNPs/SC) exhibited the significantly highest mean value for the total area of penetration. However, groups 1 (BC/SC) and 3 (BC/CWC) exhibited the lowest mean value of total penetration area, with no statistically significant difference. The integration of AgNPs with BC sealer markedly enhanced penetration into dentinal tubules. The SC technique demonstrated superior penetration relative to the CWC technique. |
| format | Article |
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| institution | OA Journals |
| issn | 2076-3417 |
| language | English |
| publishDate | 2024-12-01 |
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| spelling | doaj-art-afadbd51532243dcabc0cf31c0d4cdca2025-08-20T02:01:01ZengMDPI AGApplied Sciences2076-34172024-12-0114241163910.3390/app142411639The Impact of Silver Nanoparticles on Dentinal Tubule Penetration of Endodontic Bioceramic SealerSundus Bukhary0Sarah Alkahtany1Amal Almohaimede2Nourah Alkhayatt3Shahad Alsulaiman4Salma Alohali5Division of Endodontics, Department of Restorative Dental Science, College of Dentistry, King Saud University, Riyadh 13313, Saudi ArabiaDivision of Endodontics, Department of Restorative Dental Science, College of Dentistry, King Saud University, Riyadh 13313, Saudi ArabiaDivision of Endodontics, Department of Restorative Dental Science, College of Dentistry, King Saud University, Riyadh 13313, Saudi ArabiaUniversity Dental Hospital, King Saud University, Riyadh 13313, Saudi ArabiaUniversity Dental Hospital, King Saud University, Riyadh 13313, Saudi ArabiaUniversity Dental Hospital, King Saud University, Riyadh 13313, Saudi ArabiaThe impact of adding silver nanoparticles (AgNPs) to bioceramic (BC) sealer on their ability to penetrate dentinal tubules is still unknown. Thus, this confocal laser scanning microscopic (CLSM) study aimed to assess the extent of dentinal tubule penetration of BC sealer (TotalFill<sup>®</sup> Hiflow BC Sealer™, FKG, Switzerland) with and without AgNPs using the single-cone (SC) technique and the continuous-wave condensation (CWC) technique. AgNPs alone as well as in a mixture with the BC sealer were characterized using scanning electron microscopy and transmission electron microscopy. Single-rooted extracted human teeth (N = 100) were selected and prepared, and then divided into four groups (n = 25). Group 1 (BC/SC): BC sealer obturated with the SC technique. Group 2 (BC+AgNPs/SC): BC sealer with AgNPs obturated with the SC technique. Group 3 (BC/CWC): BC Sealer obturated with the CWC technique. Group 4 (BC+AgNPs/CWC): BC Sealer with AgNPs obturated with the CWC technique. After 2 weeks, roots were horizontally sectioned to obtain 1 mm thick dentin slices that were evaluated with CLSM. Sealer dentinal tubule penetration area and the maximum depth of penetration were measured. Data were analyzed with one-way ANOVA and the Tukey multiple comparison tests (<i>p</i> ≤ 0.05). The characterization process demonstrated a spherical-shaped nanoparticles without obvious agglomeration. The results showed that Group 2 (BC+AgNPs/SC) significantly demonstrated the highest mean tubular penetration depth, while group 3 (BC/CWC) had the lowest mean depth. Group 2 (BC+AgNPs/SC) exhibited the significantly highest mean value for the total area of penetration. However, groups 1 (BC/SC) and 3 (BC/CWC) exhibited the lowest mean value of total penetration area, with no statistically significant difference. The integration of AgNPs with BC sealer markedly enhanced penetration into dentinal tubules. The SC technique demonstrated superior penetration relative to the CWC technique.https://www.mdpi.com/2076-3417/14/24/11639nanoparticlescalcium silicate-based sealerdentin penetrationconfocal laser scanning microscope |
| spellingShingle | Sundus Bukhary Sarah Alkahtany Amal Almohaimede Nourah Alkhayatt Shahad Alsulaiman Salma Alohali The Impact of Silver Nanoparticles on Dentinal Tubule Penetration of Endodontic Bioceramic Sealer Applied Sciences nanoparticles calcium silicate-based sealer dentin penetration confocal laser scanning microscope |
| title | The Impact of Silver Nanoparticles on Dentinal Tubule Penetration of Endodontic Bioceramic Sealer |
| title_full | The Impact of Silver Nanoparticles on Dentinal Tubule Penetration of Endodontic Bioceramic Sealer |
| title_fullStr | The Impact of Silver Nanoparticles on Dentinal Tubule Penetration of Endodontic Bioceramic Sealer |
| title_full_unstemmed | The Impact of Silver Nanoparticles on Dentinal Tubule Penetration of Endodontic Bioceramic Sealer |
| title_short | The Impact of Silver Nanoparticles on Dentinal Tubule Penetration of Endodontic Bioceramic Sealer |
| title_sort | impact of silver nanoparticles on dentinal tubule penetration of endodontic bioceramic sealer |
| topic | nanoparticles calcium silicate-based sealer dentin penetration confocal laser scanning microscope |
| url | https://www.mdpi.com/2076-3417/14/24/11639 |
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