Molecular characterization and genotype of multi-drug resistant Staphylococcus epidermidis in nasal carriage of young population, Mahasarakham, Thailand
Staphylococcus epidermidis, a coagulase-negative staphylococcus, is a prevalent skin commensal that has increasingly been recognized as a significant pathogen, particularly in hospital environments, where it is associated with device-related infections. The emergence of multi-drug resistance and it...
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Association of Basic Medical Sciences of Federation of Bosnia and Herzegovina
2024-10-01
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| Series: | Biomolecules & Biomedicine |
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| Online Access: | https://www.bjbms.org/ojs/index.php/bjbms/article/view/11116 |
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| author | Peechanika Chopjitt Panita Tangthong Jiranuch Kongkaem Pritprapoan Wonkyai Achira Charoenwattanamaneechai Surasak Khankhum Phitcharat Sunthamala Anusak Kerdsin Nuchsupha Sunthamala |
| author_facet | Peechanika Chopjitt Panita Tangthong Jiranuch Kongkaem Pritprapoan Wonkyai Achira Charoenwattanamaneechai Surasak Khankhum Phitcharat Sunthamala Anusak Kerdsin Nuchsupha Sunthamala |
| author_sort | Peechanika Chopjitt |
| collection | DOAJ |
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Staphylococcus epidermidis, a coagulase-negative staphylococcus, is a prevalent skin commensal that has increasingly been recognized as a significant pathogen, particularly in hospital environments, where it is associated with device-related infections. The emergence of multi-drug resistance and its ability to form biofilms complicate the clinical management of infections caused by this organism, posing a growing public health concern. This study aimed to investigate the nasal carriage of S. epidermidis among healthy young individuals and to analyze its antibiotic resistance patterns, resistance genes, and biofilm formation capabilities. Nasal swabs were collected from 40 undergraduate students at Mahasarakham University, Thailand, aged between 20 and 22 years. A total of 38 isolates were confirmed as S. epidermidis through both phenotypic and molecular characterization. Antibiotic susceptibility testing demonstrated resistance to various classes of antimicrobials, including beta-lactams, macrolides, and tetracyclines. Notably, five isolates exhibited methicillin resistance S. epidermidis (MRSE). Resistance genes, such as mecA, ermA, tetM, tetL, and tetK, were identified across the isolates, contributing to the observed resistance profiles. Biofilm formation assays revealed that most isolates displayed weak to moderate biofilm formation, with only one isolate demonstrating strong biofilm-forming capacity. Genetic analysis indicated a significant correlation between biofilm formation and the presence of the icaA gene, which is crucial for biofilm production. This study suggests the necessity for ongoing surveillance of nasal carriage of S. epidermidis to enhance understanding of its role in the dissemination of antimicrobial resistance and biofilm-associated infections, particularly within healthcare settings.
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| format | Article |
| id | doaj-art-afada68c462e44f5b7460c73f1f9feca |
| institution | OA Journals |
| issn | 2831-0896 2831-090X |
| language | English |
| publishDate | 2024-10-01 |
| publisher | Association of Basic Medical Sciences of Federation of Bosnia and Herzegovina |
| record_format | Article |
| series | Biomolecules & Biomedicine |
| spelling | doaj-art-afada68c462e44f5b7460c73f1f9feca2025-08-20T01:47:37ZengAssociation of Basic Medical Sciences of Federation of Bosnia and HerzegovinaBiomolecules & Biomedicine2831-08962831-090X2024-10-0110.17305/bb.2024.11116Molecular characterization and genotype of multi-drug resistant Staphylococcus epidermidis in nasal carriage of young population, Mahasarakham, ThailandPeechanika Chopjitt0Panita Tangthong1Jiranuch Kongkaem2Pritprapoan Wonkyai3Achira Charoenwattanamaneechai4Surasak Khankhum5Phitcharat Sunthamala6Anusak Kerdsin7https://orcid.org/0000-0003-1055-3656Nuchsupha Sunthamala8Faculty of Public Health, Kasetsart University Chalermphrakiat, Sakon Nakhon Campus, Sakon Nakhon, ThailandDepartment of Biology, Faculty of Science, Mahasarakham University, Mahasarakham, ThailandMahasarakham University Demonstration School (Secondary), Mahasarakham University, Mahasarakham, ThailandMahasarakham University Demonstration School (Secondary), Mahasarakham University, Mahasarakham, ThailandMahasarakham University Demonstration School (Secondary), Mahasarakham University, Mahasarakham, ThailandDepartment of Biology, Faculty of Science, Mahasarakham University, Mahasarakham, ThailandDepartment of Biotechnology, Faculty of Technology, Mahasarakham University, Mahasarakham, ThailandFaculty of Public Health, Kasetsart University Chalermphrakiat, Sakon Nakhon Campus, Sakon Nakhon, ThailandDepartment of Biology, Faculty of Science, Mahasarakham University, Mahasarakham, Thailand. Staphylococcus epidermidis, a coagulase-negative staphylococcus, is a prevalent skin commensal that has increasingly been recognized as a significant pathogen, particularly in hospital environments, where it is associated with device-related infections. The emergence of multi-drug resistance and its ability to form biofilms complicate the clinical management of infections caused by this organism, posing a growing public health concern. This study aimed to investigate the nasal carriage of S. epidermidis among healthy young individuals and to analyze its antibiotic resistance patterns, resistance genes, and biofilm formation capabilities. Nasal swabs were collected from 40 undergraduate students at Mahasarakham University, Thailand, aged between 20 and 22 years. A total of 38 isolates were confirmed as S. epidermidis through both phenotypic and molecular characterization. Antibiotic susceptibility testing demonstrated resistance to various classes of antimicrobials, including beta-lactams, macrolides, and tetracyclines. Notably, five isolates exhibited methicillin resistance S. epidermidis (MRSE). Resistance genes, such as mecA, ermA, tetM, tetL, and tetK, were identified across the isolates, contributing to the observed resistance profiles. Biofilm formation assays revealed that most isolates displayed weak to moderate biofilm formation, with only one isolate demonstrating strong biofilm-forming capacity. Genetic analysis indicated a significant correlation between biofilm formation and the presence of the icaA gene, which is crucial for biofilm production. This study suggests the necessity for ongoing surveillance of nasal carriage of S. epidermidis to enhance understanding of its role in the dissemination of antimicrobial resistance and biofilm-associated infections, particularly within healthcare settings. https://www.bjbms.org/ojs/index.php/bjbms/article/view/11116Antimicrobial resistance genenasal carriageStaphylococcus epidermidismulti-drug resistantbiofilm |
| spellingShingle | Peechanika Chopjitt Panita Tangthong Jiranuch Kongkaem Pritprapoan Wonkyai Achira Charoenwattanamaneechai Surasak Khankhum Phitcharat Sunthamala Anusak Kerdsin Nuchsupha Sunthamala Molecular characterization and genotype of multi-drug resistant Staphylococcus epidermidis in nasal carriage of young population, Mahasarakham, Thailand Biomolecules & Biomedicine Antimicrobial resistance gene nasal carriage Staphylococcus epidermidis multi-drug resistant biofilm |
| title | Molecular characterization and genotype of multi-drug resistant Staphylococcus epidermidis in nasal carriage of young population, Mahasarakham, Thailand |
| title_full | Molecular characterization and genotype of multi-drug resistant Staphylococcus epidermidis in nasal carriage of young population, Mahasarakham, Thailand |
| title_fullStr | Molecular characterization and genotype of multi-drug resistant Staphylococcus epidermidis in nasal carriage of young population, Mahasarakham, Thailand |
| title_full_unstemmed | Molecular characterization and genotype of multi-drug resistant Staphylococcus epidermidis in nasal carriage of young population, Mahasarakham, Thailand |
| title_short | Molecular characterization and genotype of multi-drug resistant Staphylococcus epidermidis in nasal carriage of young population, Mahasarakham, Thailand |
| title_sort | molecular characterization and genotype of multi drug resistant staphylococcus epidermidis in nasal carriage of young population mahasarakham thailand |
| topic | Antimicrobial resistance gene nasal carriage Staphylococcus epidermidis multi-drug resistant biofilm |
| url | https://www.bjbms.org/ojs/index.php/bjbms/article/view/11116 |
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