The impact of salivary lactoperoxidase and histatin-5 on early childhood caries severity in relation to nutritional status
Introduction: Early childhood caries is a multifactorial disease. Saliva plays an important role in initiation and protection against caries, and its composition is greatly affected by nutritional status. This study was conducted to determine the impact of salivary lactoperoxidase and histatin-5 on...
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Springer
2020-12-01
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| Series: | Saudi Dental Journal |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1013905219307424 |
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| author | Shahba'a Munther |
| author_facet | Shahba'a Munther |
| author_sort | Shahba'a Munther |
| collection | DOAJ |
| description | Introduction: Early childhood caries is a multifactorial disease. Saliva plays an important role in initiation and protection against caries, and its composition is greatly affected by nutritional status. This study was conducted to determine the impact of salivary lactoperoxidase and histatin-5 on the severity of ECC in relation to nutritional status. Materials and methods: The sample consisted of 120 children aged 5 years, classified into eight groups: mild ECC in underweight children, mild ECC in normalweight children, moderate ECC in underweight children, moderate in ECC normal weight children, severe ECC in underweight children, severe ECC in normalweight, caries-free (control) underweight children and caries-free normalweight children. Each group consisted of 15 children. Stimulated saliva was collected. Salivary lactoperoxidase was analysed using Human LPO/ Lactoperoxidase ELISA Kit (CLIA)-LS-F29892, and salivary histatin-5 was analysed using Human Histatin-5 ELISA Kit MBS705083_48T. Results: Lactoperoxidase and histatin-5 concentrations were significantly higher in caries-free children than in children with ECC, and they were higher in children with mild ECC than in children with moderate ECC or in children with severe ECC. They were significantly higher among children with normal weight than among those who were underweight (p < 0.01). ECC and nutritional status recorded non-significant interactions with both LPO and HST-5 (p > 0.01), but there was significant interaction between these two variables and LPO and HST-5 together (p < 0.01). The Pearson's correlation coefficient test recorded significant negative correlations between ECC severity and both salivary lactoperoxidase and histatin-5 among the eight study groups, whereas significant positive correlations were recorded between BMI values and both salivary lactoperoxidase and histatin-5 among the eight study groups. Conclusion: Salivary lactoperoxidase and histatin-5 may be affected by nutritional status, and these two parameters may play an important role in caries prevention at high concentrations. There is interaction between these two parameters and ECC severity and nutrition. |
| format | Article |
| id | doaj-art-afa7fc46815f489294c3fc12a462cb20 |
| institution | Kabale University |
| issn | 1013-9052 |
| language | English |
| publishDate | 2020-12-01 |
| publisher | Springer |
| record_format | Article |
| series | Saudi Dental Journal |
| spelling | doaj-art-afa7fc46815f489294c3fc12a462cb202025-08-20T03:39:04ZengSpringerSaudi Dental Journal1013-90522020-12-0132841041610.1016/j.sdentj.2020.01.010The impact of salivary lactoperoxidase and histatin-5 on early childhood caries severity in relation to nutritional statusShahba'a Munther0Department of Pedodontic and Preventive Dentistry, College of Dentistry, University of Baghdad, IraqIntroduction: Early childhood caries is a multifactorial disease. Saliva plays an important role in initiation and protection against caries, and its composition is greatly affected by nutritional status. This study was conducted to determine the impact of salivary lactoperoxidase and histatin-5 on the severity of ECC in relation to nutritional status. Materials and methods: The sample consisted of 120 children aged 5 years, classified into eight groups: mild ECC in underweight children, mild ECC in normalweight children, moderate ECC in underweight children, moderate in ECC normal weight children, severe ECC in underweight children, severe ECC in normalweight, caries-free (control) underweight children and caries-free normalweight children. Each group consisted of 15 children. Stimulated saliva was collected. Salivary lactoperoxidase was analysed using Human LPO/ Lactoperoxidase ELISA Kit (CLIA)-LS-F29892, and salivary histatin-5 was analysed using Human Histatin-5 ELISA Kit MBS705083_48T. Results: Lactoperoxidase and histatin-5 concentrations were significantly higher in caries-free children than in children with ECC, and they were higher in children with mild ECC than in children with moderate ECC or in children with severe ECC. They were significantly higher among children with normal weight than among those who were underweight (p < 0.01). ECC and nutritional status recorded non-significant interactions with both LPO and HST-5 (p > 0.01), but there was significant interaction between these two variables and LPO and HST-5 together (p < 0.01). The Pearson's correlation coefficient test recorded significant negative correlations between ECC severity and both salivary lactoperoxidase and histatin-5 among the eight study groups, whereas significant positive correlations were recorded between BMI values and both salivary lactoperoxidase and histatin-5 among the eight study groups. Conclusion: Salivary lactoperoxidase and histatin-5 may be affected by nutritional status, and these two parameters may play an important role in caries prevention at high concentrations. There is interaction between these two parameters and ECC severity and nutrition.http://www.sciencedirect.com/science/article/pii/S1013905219307424Early childhood caries severityNutritional statusSalivary lactoperoxidaseSalivary histatin-5 |
| spellingShingle | Shahba'a Munther The impact of salivary lactoperoxidase and histatin-5 on early childhood caries severity in relation to nutritional status Saudi Dental Journal Early childhood caries severity Nutritional status Salivary lactoperoxidase Salivary histatin-5 |
| title | The impact of salivary lactoperoxidase and histatin-5 on early childhood caries severity in relation to nutritional status |
| title_full | The impact of salivary lactoperoxidase and histatin-5 on early childhood caries severity in relation to nutritional status |
| title_fullStr | The impact of salivary lactoperoxidase and histatin-5 on early childhood caries severity in relation to nutritional status |
| title_full_unstemmed | The impact of salivary lactoperoxidase and histatin-5 on early childhood caries severity in relation to nutritional status |
| title_short | The impact of salivary lactoperoxidase and histatin-5 on early childhood caries severity in relation to nutritional status |
| title_sort | impact of salivary lactoperoxidase and histatin 5 on early childhood caries severity in relation to nutritional status |
| topic | Early childhood caries severity Nutritional status Salivary lactoperoxidase Salivary histatin-5 |
| url | http://www.sciencedirect.com/science/article/pii/S1013905219307424 |
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