Evolution of Microbial Community Structure and Denitrifying Functional Microorganisms in the Biological Sponge Iron System
With the growing problem of global water pollution, nitrogen pollution has become a key factor affecting aquatic ecosystems and human health. The biological sponge iron system (BSIS) has gained attention as a research hotspot due to its efficient denitrification capability. This study focused on the...
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2025-06-01
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| author | Jing Li Huina Xie Wei Zhao Jie Li |
| author_facet | Jing Li Huina Xie Wei Zhao Jie Li |
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| collection | DOAJ |
| description | With the growing problem of global water pollution, nitrogen pollution has become a key factor affecting aquatic ecosystems and human health. The biological sponge iron system (BSIS) has gained attention as a research hotspot due to its efficient denitrification capability. This study focused on the changes in microbial community structure and the relative abundance and interrelationships of nitrogen cycle-related functional bacteria at different operational stages of the BSIS with a sponge iron (SFe) dosage of 90 g/L. The results showed that as the operation time of the reactor extended, the relative abundance of denitrifying genera such as <i>Saccharimonadales</i>, <i>Arenimonas</i>, and Acinetobacter significantly increased, while the relative abundance of Proteobacteria showed a trend of initial increase followed by a decrease. The relative abundance of nitrifying bacteria exhibited a more complex variation, whereas the abundance of denitrifying bacteria showed a continuous upward trend. In addition, there were complex interrelationships among different denitrifying bacteria, such as a positive correlation between <i>Saccharimonadales</i> and <i>Acetobacteraceae</i>, and a negative correlation between <i>Rhodothermus</i> and <i>Pseudoxanthomonas</i>. This study not only revealed the changes in the relative abundance and interrelationships of microbial communities and nitrogen cycle-related functional bacteria over time with an SFe dosage of 90 g/L, but also provided a new perspective for understanding the intrinsic mechanism of enhanced biological denitrification by sponge iron. These findings are of great significance for optimizing the operating parameters of the BSIS, improving denitrification efficiency, and promoting the practical application of this technology in the field of environmental engineering. |
| format | Article |
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| institution | DOAJ |
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| publishDate | 2025-06-01 |
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| spelling | doaj-art-af9dca69982542e9a9785ef3ec82e57d2025-08-20T03:16:46ZengMDPI AGApplied Sciences2076-34172025-06-011513724410.3390/app15137244Evolution of Microbial Community Structure and Denitrifying Functional Microorganisms in the Biological Sponge Iron SystemJing Li0Huina Xie1Wei Zhao2Jie Li3College of Environmental and Municipal Engineering, Lanzhou Jiaotong University, Lanzhou 730070, ChinaCollege of Environmental and Municipal Engineering, Lanzhou Jiaotong University, Lanzhou 730070, ChinaCollege of Environmental and Municipal Engineering, Lanzhou Jiaotong University, Lanzhou 730070, ChinaCollege of Environmental and Municipal Engineering, Lanzhou Jiaotong University, Lanzhou 730070, ChinaWith the growing problem of global water pollution, nitrogen pollution has become a key factor affecting aquatic ecosystems and human health. The biological sponge iron system (BSIS) has gained attention as a research hotspot due to its efficient denitrification capability. This study focused on the changes in microbial community structure and the relative abundance and interrelationships of nitrogen cycle-related functional bacteria at different operational stages of the BSIS with a sponge iron (SFe) dosage of 90 g/L. The results showed that as the operation time of the reactor extended, the relative abundance of denitrifying genera such as <i>Saccharimonadales</i>, <i>Arenimonas</i>, and Acinetobacter significantly increased, while the relative abundance of Proteobacteria showed a trend of initial increase followed by a decrease. The relative abundance of nitrifying bacteria exhibited a more complex variation, whereas the abundance of denitrifying bacteria showed a continuous upward trend. In addition, there were complex interrelationships among different denitrifying bacteria, such as a positive correlation between <i>Saccharimonadales</i> and <i>Acetobacteraceae</i>, and a negative correlation between <i>Rhodothermus</i> and <i>Pseudoxanthomonas</i>. This study not only revealed the changes in the relative abundance and interrelationships of microbial communities and nitrogen cycle-related functional bacteria over time with an SFe dosage of 90 g/L, but also provided a new perspective for understanding the intrinsic mechanism of enhanced biological denitrification by sponge iron. These findings are of great significance for optimizing the operating parameters of the BSIS, improving denitrification efficiency, and promoting the practical application of this technology in the field of environmental engineering.https://www.mdpi.com/2076-3417/15/13/7244bio-sponge iron systemmicrobial community structuredenitrifying functional microorganismsevolution |
| spellingShingle | Jing Li Huina Xie Wei Zhao Jie Li Evolution of Microbial Community Structure and Denitrifying Functional Microorganisms in the Biological Sponge Iron System Applied Sciences bio-sponge iron system microbial community structure denitrifying functional microorganisms evolution |
| title | Evolution of Microbial Community Structure and Denitrifying Functional Microorganisms in the Biological Sponge Iron System |
| title_full | Evolution of Microbial Community Structure and Denitrifying Functional Microorganisms in the Biological Sponge Iron System |
| title_fullStr | Evolution of Microbial Community Structure and Denitrifying Functional Microorganisms in the Biological Sponge Iron System |
| title_full_unstemmed | Evolution of Microbial Community Structure and Denitrifying Functional Microorganisms in the Biological Sponge Iron System |
| title_short | Evolution of Microbial Community Structure and Denitrifying Functional Microorganisms in the Biological Sponge Iron System |
| title_sort | evolution of microbial community structure and denitrifying functional microorganisms in the biological sponge iron system |
| topic | bio-sponge iron system microbial community structure denitrifying functional microorganisms evolution |
| url | https://www.mdpi.com/2076-3417/15/13/7244 |
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