Regulatory mechanism of exogenous myo-inositol improving high temperature stress tolerance of tomato seedlings
[Objective] Studing the effect of exogenous myo-inositol on the physiological and biochemical indexes of tomato seedlings and the molecular mechanism of tolerance to high temperature stress will provide a theoretical basis for the improvement of heat resistance of tomato. [Methods] Tomato variety...
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| Format: | Article |
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Science Press
2024-12-01
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| Series: | Xibei zhiwu xuebao |
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| Online Access: | http://xbzwxb.alljournal.net/xbzwxb/article/abstract/20240404?st=article_issue |
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| author | CHEN Yan HAN Liyuan DU Zilong ZHU Dongxing ZHU Weimin ZHANG Yingying |
| author_facet | CHEN Yan HAN Liyuan DU Zilong ZHU Dongxing ZHU Weimin ZHANG Yingying |
| author_sort | CHEN Yan |
| collection | DOAJ |
| description | [Objective] Studing the effect of exogenous myo-inositol on the physiological and biochemical indexes
of tomato seedlings and the molecular mechanism of tolerance to high temperature stress will provide
a theoretical basis for the improvement of heat resistance of tomato. [Methods] Tomato variety ‘NRP20-
7’ was used as the test material, and water and myo-inositol were sprayed separately at normal temperature
and high temperature, respectively. The phenotypic changes under treatments were analyzed by hyperspectral
imaging, and the physiological indexes of each treatment group were analyzed. The expression
of heat responsive genes, antioxidant, and ABA-related genes were analyzed by qRT-PCR. [Results] 20
mmol/L myo-inositol treatment could effectively alleviate the damage of high temperature stress on tomato
seedlings, reduce the heat damage index of tomato leaves, increase the maximum photochemical efficiency
(Fv/Fm), and significantly increased the content of photosynthetic pigments. Exogenous myo-inositol increased
proline and soluble sugar contents and peroxidase activity, and decreased malondialdehyde, hydrogen
peroxide, and superoxide anion contents in tomato seedlings at high temperature. Exogenous myo-inositol
increased the expression of heat response genes, antioxidant-related genes, ascorbic acid synthesis
genes, and decreased the expression of heat shock protein genes. In addition, exogenous myo-inositol responded
to high temperature stress by regulating abscisic acid synthesis and signaling pathways to regulate
the level of ABA in tomato seedlings. [Conclusion] Exogenous myo-inositol enhances tomato tolerance to
high temperature stress by regulating complex biological processes in plants. |
| format | Article |
| id | doaj-art-af7713e0120043ac8d731cfd3d89576d |
| institution | Kabale University |
| issn | 1000-4025 |
| language | zho |
| publishDate | 2024-12-01 |
| publisher | Science Press |
| record_format | Article |
| series | Xibei zhiwu xuebao |
| spelling | doaj-art-af7713e0120043ac8d731cfd3d89576d2025-02-10T12:15:12ZzhoScience PressXibei zhiwu xuebao1000-40252024-12-0144121856186710.7606/j.issn.1000-4025.20240404Regulatory mechanism of exogenous myo-inositol improving high temperature stress tolerance of tomato seedlingsCHEN Yan0HAN Liyuan1DU Zilong2ZHU Dongxing3ZHU Weimin4ZHANG Yingying5College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China; Shanghai Key Laboratory of Facility Horticulture Technology, Facility Horticulture Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201403, ChinaShanghai Key Laboratory of Facility Horticulture Technology, Facility Horticulture Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201403, ChinaShanghai Key Laboratory of Facility Horticulture Technology, Facility Horticulture Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201403, ChinaShanghai Jinshan District Zhangyan Town Economic Development Service Center, Shanghai 201514, ChinaCollege of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China; Shanghai Key Laboratory of Facility Horticulture Technology, Facility Horticulture Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201403, ChinaShanghai Key Laboratory of Facility Horticulture Technology, Facility Horticulture Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China[Objective] Studing the effect of exogenous myo-inositol on the physiological and biochemical indexes of tomato seedlings and the molecular mechanism of tolerance to high temperature stress will provide a theoretical basis for the improvement of heat resistance of tomato. [Methods] Tomato variety ‘NRP20- 7’ was used as the test material, and water and myo-inositol were sprayed separately at normal temperature and high temperature, respectively. The phenotypic changes under treatments were analyzed by hyperspectral imaging, and the physiological indexes of each treatment group were analyzed. The expression of heat responsive genes, antioxidant, and ABA-related genes were analyzed by qRT-PCR. [Results] 20 mmol/L myo-inositol treatment could effectively alleviate the damage of high temperature stress on tomato seedlings, reduce the heat damage index of tomato leaves, increase the maximum photochemical efficiency (Fv/Fm), and significantly increased the content of photosynthetic pigments. Exogenous myo-inositol increased proline and soluble sugar contents and peroxidase activity, and decreased malondialdehyde, hydrogen peroxide, and superoxide anion contents in tomato seedlings at high temperature. Exogenous myo-inositol increased the expression of heat response genes, antioxidant-related genes, ascorbic acid synthesis genes, and decreased the expression of heat shock protein genes. In addition, exogenous myo-inositol responded to high temperature stress by regulating abscisic acid synthesis and signaling pathways to regulate the level of ABA in tomato seedlings. [Conclusion] Exogenous myo-inositol enhances tomato tolerance to high temperature stress by regulating complex biological processes in plants.http://xbzwxb.alljournal.net/xbzwxb/article/abstract/20240404?st=article_issuetomatohigh temperature stressmyo-inositolstress resistancegene expression |
| spellingShingle | CHEN Yan HAN Liyuan DU Zilong ZHU Dongxing ZHU Weimin ZHANG Yingying Regulatory mechanism of exogenous myo-inositol improving high temperature stress tolerance of tomato seedlings Xibei zhiwu xuebao tomato high temperature stress myo-inositol stress resistance gene expression |
| title | Regulatory mechanism of exogenous myo-inositol improving high temperature stress tolerance of tomato seedlings |
| title_full | Regulatory mechanism of exogenous myo-inositol improving high temperature stress tolerance of tomato seedlings |
| title_fullStr | Regulatory mechanism of exogenous myo-inositol improving high temperature stress tolerance of tomato seedlings |
| title_full_unstemmed | Regulatory mechanism of exogenous myo-inositol improving high temperature stress tolerance of tomato seedlings |
| title_short | Regulatory mechanism of exogenous myo-inositol improving high temperature stress tolerance of tomato seedlings |
| title_sort | regulatory mechanism of exogenous myo inositol improving high temperature stress tolerance of tomato seedlings |
| topic | tomato high temperature stress myo-inositol stress resistance gene expression |
| url | http://xbzwxb.alljournal.net/xbzwxb/article/abstract/20240404?st=article_issue |
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