Internal Standards for Quantitative Analysis of Chemical Warfare Agents by the GC/MS Method: Nerve Agents

General conditions and requirements for an internal standard useful in the determination of chemical warfare agents (CWAs) by the method of gas chromatography coupled with mass detection (GC/MS) were defined. The determination is based on a GC/MS analysis of a mixture of a CWA with an internal stand...

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Main Authors: Tomas Capoun, Jana Krykorkova
Format: Article
Language:English
Published: Wiley 2020-01-01
Series:Journal of Analytical Methods in Chemistry
Online Access:http://dx.doi.org/10.1155/2020/8857210
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author Tomas Capoun
Jana Krykorkova
author_facet Tomas Capoun
Jana Krykorkova
author_sort Tomas Capoun
collection DOAJ
description General conditions and requirements for an internal standard useful in the determination of chemical warfare agents (CWAs) by the method of gas chromatography coupled with mass detection (GC/MS) were defined. The determination is based on a GC/MS analysis of a mixture of a CWA with an internal standard, conversion of the TIC chromatogram to a chromatogram extracted at a particular m/z ratio, and calculation of the CWA concentration from the internal standard concentration, response factor, and chromatographic peak areas. Available internal standards were identified, and they were verified for seven organophosphorus nerve-paralysing agents. Corresponding response factors were determined as a ratio of gradients of the linear functions of the peak area and compound concentration. Linearity, repeatability, and accuracy of the measurements were evaluated. The determination can be performed on all GC/MS systems of the Fire Rescue Service of the Czech Republic (FRS), where no CWA standards are available.
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spelling doaj-art-af4b71b0b194435cbb7b5a7f1200cc9f2025-08-20T02:19:07ZengWileyJournal of Analytical Methods in Chemistry2090-88652090-88732020-01-01202010.1155/2020/88572108857210Internal Standards for Quantitative Analysis of Chemical Warfare Agents by the GC/MS Method: Nerve AgentsTomas Capoun0Jana Krykorkova1Ministry of Interior—Directorate General of the Fire Rescue Service CR, Population Protection Institute, Na Luzci 204, Lazne Bohdanec 533 41, Czech RepublicMinistry of Interior—Directorate General of the Fire Rescue Service CR, Population Protection Institute, Na Luzci 204, Lazne Bohdanec 533 41, Czech RepublicGeneral conditions and requirements for an internal standard useful in the determination of chemical warfare agents (CWAs) by the method of gas chromatography coupled with mass detection (GC/MS) were defined. The determination is based on a GC/MS analysis of a mixture of a CWA with an internal standard, conversion of the TIC chromatogram to a chromatogram extracted at a particular m/z ratio, and calculation of the CWA concentration from the internal standard concentration, response factor, and chromatographic peak areas. Available internal standards were identified, and they were verified for seven organophosphorus nerve-paralysing agents. Corresponding response factors were determined as a ratio of gradients of the linear functions of the peak area and compound concentration. Linearity, repeatability, and accuracy of the measurements were evaluated. The determination can be performed on all GC/MS systems of the Fire Rescue Service of the Czech Republic (FRS), where no CWA standards are available.http://dx.doi.org/10.1155/2020/8857210
spellingShingle Tomas Capoun
Jana Krykorkova
Internal Standards for Quantitative Analysis of Chemical Warfare Agents by the GC/MS Method: Nerve Agents
Journal of Analytical Methods in Chemistry
title Internal Standards for Quantitative Analysis of Chemical Warfare Agents by the GC/MS Method: Nerve Agents
title_full Internal Standards for Quantitative Analysis of Chemical Warfare Agents by the GC/MS Method: Nerve Agents
title_fullStr Internal Standards for Quantitative Analysis of Chemical Warfare Agents by the GC/MS Method: Nerve Agents
title_full_unstemmed Internal Standards for Quantitative Analysis of Chemical Warfare Agents by the GC/MS Method: Nerve Agents
title_short Internal Standards for Quantitative Analysis of Chemical Warfare Agents by the GC/MS Method: Nerve Agents
title_sort internal standards for quantitative analysis of chemical warfare agents by the gc ms method nerve agents
url http://dx.doi.org/10.1155/2020/8857210
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