Heterologous Expression and Enzymatic Properties of β-Glucuronidase from <i>Clostridium perfringens</i> and Its Application in Bilirubin Transformation

Heterologous Expression and Enzymatic Properties of β-Glucuronidase from <i>Clostridium perfringens</i> and Its Application in Bilirubin Transformation

β-glucuronidase is an important hydrolase, which plays an important role in drug metabolism, clinical diagnostics, and biotransformation. This study focuses on the heterologous expression, isolation, purification, and its enzymatic properties of β-glucuronidase CpGUS from <i>Clostridium perfri...

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Bibliographic Details
Main Authors: Qianlin Wu, Qing Guo, Fo Yang, Mengru Li, Yumeng Zhu, Binpeng Xu, Lu Zhao, Shanshan Zhang, Youyu Xie, Feng Li, Xiaomin Wu, Dayong Xu
Format: Article
Language:English
Published: MDPI AG 2025-04-01
Series:Microorganisms
Subjects:
β-glucuronidase CpGUS
bilirubin
molecular dynamics simulation
catalytic mechanism
Online Access:https://www.mdpi.com/2076-2607/13/5/1043
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