Production of antihypertensive and antidiabetic peptide fractions from quinoa (Chenopodium quinoa Willd.) by electrodialysis with ultrafiltration membranes
Processing bioactive peptides from natural sources using electrodialysis with ultrafiltration membranes (EDUF) have gained attention since it can fractionate in terms of their charge and molecular weight. Quinoa is a pseudo-cereal highlighted by its high protein content, amino acid profile and adapt...
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Tsinghua University Press
2022-11-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213453022001070 |
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| author | Adrián González-Muñoz Marion Valle Rotimi E. Aluko Laurent Bazinet Javier Enrione |
| author_facet | Adrián González-Muñoz Marion Valle Rotimi E. Aluko Laurent Bazinet Javier Enrione |
| author_sort | Adrián González-Muñoz |
| collection | DOAJ |
| description | Processing bioactive peptides from natural sources using electrodialysis with ultrafiltration membranes (EDUF) have gained attention since it can fractionate in terms of their charge and molecular weight. Quinoa is a pseudo-cereal highlighted by its high protein content, amino acid profile and adapting growing conditions. The present work aimed at the production of quinoa peptides through fractionation using EDUF and to test the fractions according to antihypertensive and antidiabetic activity. Experimental data showed the production of peptides ranging between 0.4 and 1.5 kDa. Cationic (CQPF) (3.01 %), anionic (AQPF) (1.18 %) and the electrically neutral fraction quinoa protein hydrolysate (QPH)-EDUF (∼95 %) were obtained. In-vitro studies showed the highest glucose uptake modulation in L6 cell skeletal myoblasts in presence of QPH-EDUF and AQPF (17 % and 11 %) indicating potential antidiabetic activity. The antihypertensive effect studied in-vivo in spontaneously hypertensive rats (SHR), showed a decrease in systolic blood pressure in presence of the fractionated peptides, being 100 mg/kg a dose comparable to Captopril (positive control). These results contribute to the current knowledge of bioactive peptides from quinoa by reporting the relevance of EDUF as tool to produce selected peptide fractions. Nevertheless, further characterization is needed towards peptide sequencing, their respective role in the metabolism and scaling-up production using EDUF. |
| format | Article |
| id | doaj-art-af4941d6059a44acaf486197c9a0b96c |
| institution | Kabale University |
| issn | 2213-4530 |
| language | English |
| publishDate | 2022-11-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-af4941d6059a44acaf486197c9a0b96c2025-02-03T05:38:59ZengTsinghua University PressFood Science and Human Wellness2213-45302022-11-0111616501659Production of antihypertensive and antidiabetic peptide fractions from quinoa (Chenopodium quinoa Willd.) by electrodialysis with ultrafiltration membranesAdrián González-Muñoz0Marion Valle1Rotimi E. Aluko2Laurent Bazinet3Javier Enrione4Biopolymer Research and Engineering Lab (BiopREL), School of Nutrition and Dietetics, Faculty of Medicine, Universidad de los Andes, Monseñor Álvaro del Portillo 12455, Chile; Centre for Biomedical Research and Innovation (CIIB), Universidad de los Andes, Chile, Monseñor Álvaro del Portillo 12455, ChileInstitute of Nutrition and Functional Foods (INAF), Université Laval, Québec QC G1V 0A6, Canada; Department of Anatomy and Physiology, Faculty of Medicine, Laval Hospital Research Center, Université Laval, Québec QC G1V 4G5, CanadaDepartment of Food and Human Nutritional Sciences, University of Manitoba, Winnipeg MB R3T 2N2, CanadaInstitute of Nutrition and Functional Foods (INAF), Université Laval, Québec QC G1V 0A6, Canada; Department of Food Sciences, Laboratoire de Transformation Alimentaire et Procédés ÉlectroMembranaire (LTAPEM, Laboratory of Food Processing and Electro-Membrane Processes), Université Laval, Québec QC G1V 0A6, Canada; Corresponding author at: Institute of Nutrition and Functional Foods (INAF), Université Laval, Québec QC G1V 0A6, CanadaBiopolymer Research and Engineering Lab (BiopREL), School of Nutrition and Dietetics, Faculty of Medicine, Universidad de los Andes, Monseñor Álvaro del Portillo 12455, Chile; Centre for Biomedical Research and Innovation (CIIB), Universidad de los Andes, Chile, Monseñor Álvaro del Portillo 12455, Chile; Corresponding author at: Biopolymer Research and Engineering Lab (BiopREL), School of Nutrition and Dietetics, Faculty of Medicine, Universidad de los Andes, Monseñor Álvaro del Portillo 12455, ChileProcessing bioactive peptides from natural sources using electrodialysis with ultrafiltration membranes (EDUF) have gained attention since it can fractionate in terms of their charge and molecular weight. Quinoa is a pseudo-cereal highlighted by its high protein content, amino acid profile and adapting growing conditions. The present work aimed at the production of quinoa peptides through fractionation using EDUF and to test the fractions according to antihypertensive and antidiabetic activity. Experimental data showed the production of peptides ranging between 0.4 and 1.5 kDa. Cationic (CQPF) (3.01 %), anionic (AQPF) (1.18 %) and the electrically neutral fraction quinoa protein hydrolysate (QPH)-EDUF (∼95 %) were obtained. In-vitro studies showed the highest glucose uptake modulation in L6 cell skeletal myoblasts in presence of QPH-EDUF and AQPF (17 % and 11 %) indicating potential antidiabetic activity. The antihypertensive effect studied in-vivo in spontaneously hypertensive rats (SHR), showed a decrease in systolic blood pressure in presence of the fractionated peptides, being 100 mg/kg a dose comparable to Captopril (positive control). These results contribute to the current knowledge of bioactive peptides from quinoa by reporting the relevance of EDUF as tool to produce selected peptide fractions. Nevertheless, further characterization is needed towards peptide sequencing, their respective role in the metabolism and scaling-up production using EDUF.http://www.sciencedirect.com/science/article/pii/S2213453022001070QuinoaElectrodialysis with ultrafiltration membranesBioactive peptidesAntihypertensiveAntidiabetic |
| spellingShingle | Adrián González-Muñoz Marion Valle Rotimi E. Aluko Laurent Bazinet Javier Enrione Production of antihypertensive and antidiabetic peptide fractions from quinoa (Chenopodium quinoa Willd.) by electrodialysis with ultrafiltration membranes Food Science and Human Wellness Quinoa Electrodialysis with ultrafiltration membranes Bioactive peptides Antihypertensive Antidiabetic |
| title | Production of antihypertensive and antidiabetic peptide fractions from quinoa (Chenopodium quinoa Willd.) by electrodialysis with ultrafiltration membranes |
| title_full | Production of antihypertensive and antidiabetic peptide fractions from quinoa (Chenopodium quinoa Willd.) by electrodialysis with ultrafiltration membranes |
| title_fullStr | Production of antihypertensive and antidiabetic peptide fractions from quinoa (Chenopodium quinoa Willd.) by electrodialysis with ultrafiltration membranes |
| title_full_unstemmed | Production of antihypertensive and antidiabetic peptide fractions from quinoa (Chenopodium quinoa Willd.) by electrodialysis with ultrafiltration membranes |
| title_short | Production of antihypertensive and antidiabetic peptide fractions from quinoa (Chenopodium quinoa Willd.) by electrodialysis with ultrafiltration membranes |
| title_sort | production of antihypertensive and antidiabetic peptide fractions from quinoa chenopodium quinoa willd by electrodialysis with ultrafiltration membranes |
| topic | Quinoa Electrodialysis with ultrafiltration membranes Bioactive peptides Antihypertensive Antidiabetic |
| url | http://www.sciencedirect.com/science/article/pii/S2213453022001070 |
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