Development and Characterization of Printlets of Lamivudine for Pediatric Patients Using Selective Laser Sintering

<b>Background:</b> Lamivudine is widely used alone or in combination with other anti-HIV drugs in the infant to adolescent age groups of pediatric populations. Compounding of medications is frequently used for pediatric patients. However, many issues have been reported for the compounded...

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Main Authors: Canberk Kayalar, Swaroop Jalandar Pansare, Gereziher Sibhat, Mathew Kuttolamadom, Ziyaur Rahman, Mansoor A. Khan
Format: Article
Language:English
Published: MDPI AG 2025-03-01
Series:Pharmaceuticals
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Online Access:https://www.mdpi.com/1424-8247/18/3/356
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author Canberk Kayalar
Swaroop Jalandar Pansare
Gereziher Sibhat
Mathew Kuttolamadom
Ziyaur Rahman
Mansoor A. Khan
author_facet Canberk Kayalar
Swaroop Jalandar Pansare
Gereziher Sibhat
Mathew Kuttolamadom
Ziyaur Rahman
Mansoor A. Khan
author_sort Canberk Kayalar
collection DOAJ
description <b>Background:</b> Lamivudine is widely used alone or in combination with other anti-HIV drugs in the infant to adolescent age groups of pediatric populations. Compounding of medications is frequently used for pediatric patients. However, many issues have been reported for the compounded formulation such as assay, stability, safety, and efficacy. Three-dimensional printing can overcome these issues. <b>Objective:</b> The aim of this study was to understand the effect of process and formulation variables on lamivudine printlets for pediatric populations using selective laser sintering. <b>Methods:</b> The Plackett–Burman screening design was used to prepare 12 formulations to study six variables, namely, laser scanning speed (130–150 °C), surface temperature (105–120 °C), chamber temperature (250–350 mm/s), sucrose (0–30%), hydroxypropyl methylcellulose (0–42%), and Kollidon<sup>®</sup> CL-M (0–5%). The formulations were tested for dissolution, disintegration, hardness, assay, X-ray diffraction, differential scanning calorimetry, stability, and pharmacokinetics in Sprague Dawley rats. <b>Results:</b> The assay of the printlet formulations varied between 93.1 and 103.5% and the disintegration time was 2.8 ± 1.2 (F1) to 43.7 ± 2.7 (F10) s. Due to high surface temperatures, the unsintered powder in the printing chamber experienced significant changes in crystallinity. No statistical significance was observed between the pharmacokinetic parameters of the printlets and commercial tablets (<i>p</i> > 0.05). The maximum plasma concentration (C<sub>max</sub>), time to reach maximum plasma concentration (T<sub>max</sub>), and area under the curve (AUC) of the printlets and commercial tablets were 295.5 ± 33.0 and 305.0 ± 70.1 ng/mL, 0.5 ± 0.0 and 1.0 ± 0.8 h, and 1414.1 ± 174.0 and 1987.2 ± 700.5 ng.h/mL, respectively. <b>Conclusions:</b> In summary, fast-disintegrating and dissolving 3D printed lamivudine was found to be bioequivalent to commercial formulation of lamivudine. Thus, it is a viable method for dispensing personalized lamivudine printlets for pediatric populations.
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spelling doaj-art-af3d69ca527e4c4ead699ce334fb8e612025-08-20T02:10:24ZengMDPI AGPharmaceuticals1424-82472025-03-0118335610.3390/ph18030356Development and Characterization of Printlets of Lamivudine for Pediatric Patients Using Selective Laser SinteringCanberk Kayalar0Swaroop Jalandar Pansare1Gereziher Sibhat2Mathew Kuttolamadom3Ziyaur Rahman4Mansoor A. Khan5Irma Lerma Rangel College of Pharmacy, Texas A&M Health Science Center, Texas A&M University, College Station, TX 77843, USAIrma Lerma Rangel College of Pharmacy, Texas A&M Health Science Center, Texas A&M University, College Station, TX 77843, USAIrma Lerma Rangel College of Pharmacy, Texas A&M Health Science Center, Texas A&M University, College Station, TX 77843, USAThe Department of Engineering Technology and Industrial Distribution, Texas A&M University, College Station, TX 77843, USAIrma Lerma Rangel College of Pharmacy, Texas A&M Health Science Center, Texas A&M University, College Station, TX 77843, USAIrma Lerma Rangel College of Pharmacy, Texas A&M Health Science Center, Texas A&M University, College Station, TX 77843, USA<b>Background:</b> Lamivudine is widely used alone or in combination with other anti-HIV drugs in the infant to adolescent age groups of pediatric populations. Compounding of medications is frequently used for pediatric patients. However, many issues have been reported for the compounded formulation such as assay, stability, safety, and efficacy. Three-dimensional printing can overcome these issues. <b>Objective:</b> The aim of this study was to understand the effect of process and formulation variables on lamivudine printlets for pediatric populations using selective laser sintering. <b>Methods:</b> The Plackett–Burman screening design was used to prepare 12 formulations to study six variables, namely, laser scanning speed (130–150 °C), surface temperature (105–120 °C), chamber temperature (250–350 mm/s), sucrose (0–30%), hydroxypropyl methylcellulose (0–42%), and Kollidon<sup>®</sup> CL-M (0–5%). The formulations were tested for dissolution, disintegration, hardness, assay, X-ray diffraction, differential scanning calorimetry, stability, and pharmacokinetics in Sprague Dawley rats. <b>Results:</b> The assay of the printlet formulations varied between 93.1 and 103.5% and the disintegration time was 2.8 ± 1.2 (F1) to 43.7 ± 2.7 (F10) s. Due to high surface temperatures, the unsintered powder in the printing chamber experienced significant changes in crystallinity. No statistical significance was observed between the pharmacokinetic parameters of the printlets and commercial tablets (<i>p</i> > 0.05). The maximum plasma concentration (C<sub>max</sub>), time to reach maximum plasma concentration (T<sub>max</sub>), and area under the curve (AUC) of the printlets and commercial tablets were 295.5 ± 33.0 and 305.0 ± 70.1 ng/mL, 0.5 ± 0.0 and 1.0 ± 0.8 h, and 1414.1 ± 174.0 and 1987.2 ± 700.5 ng.h/mL, respectively. <b>Conclusions:</b> In summary, fast-disintegrating and dissolving 3D printed lamivudine was found to be bioequivalent to commercial formulation of lamivudine. Thus, it is a viable method for dispensing personalized lamivudine printlets for pediatric populations.https://www.mdpi.com/1424-8247/18/3/356lamivudineselective laser sinteringprintletsdissolutionpharmacokinetics
spellingShingle Canberk Kayalar
Swaroop Jalandar Pansare
Gereziher Sibhat
Mathew Kuttolamadom
Ziyaur Rahman
Mansoor A. Khan
Development and Characterization of Printlets of Lamivudine for Pediatric Patients Using Selective Laser Sintering
Pharmaceuticals
lamivudine
selective laser sintering
printlets
dissolution
pharmacokinetics
title Development and Characterization of Printlets of Lamivudine for Pediatric Patients Using Selective Laser Sintering
title_full Development and Characterization of Printlets of Lamivudine for Pediatric Patients Using Selective Laser Sintering
title_fullStr Development and Characterization of Printlets of Lamivudine for Pediatric Patients Using Selective Laser Sintering
title_full_unstemmed Development and Characterization of Printlets of Lamivudine for Pediatric Patients Using Selective Laser Sintering
title_short Development and Characterization of Printlets of Lamivudine for Pediatric Patients Using Selective Laser Sintering
title_sort development and characterization of printlets of lamivudine for pediatric patients using selective laser sintering
topic lamivudine
selective laser sintering
printlets
dissolution
pharmacokinetics
url https://www.mdpi.com/1424-8247/18/3/356
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