Combination with Methotrexate and Cyclophosphamide Attenuated Maturation of Dendritic Cells: Inducing Treg Skewing and Th17 Suppression In Vivo
Immune disorder is considered the main pathogenesis of autoimmune diseases, such as rheumatoid arthritis (RA). The balance of the two special subsets of CD4+T cells, T helper cell 17 (Th17), and Regulator T cell (Treg) is the key factor of maintaining a normal immune response. Dendritic cells (DCs),...
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | Clinical and Developmental Immunology |
| Online Access: | http://dx.doi.org/10.1155/2013/238035 |
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| author | Xiaoyang Yu Caihong Wang Jing Luo Xiangcong Zhao Lixing Wang Xiaofeng Li |
| author_facet | Xiaoyang Yu Caihong Wang Jing Luo Xiangcong Zhao Lixing Wang Xiaofeng Li |
| author_sort | Xiaoyang Yu |
| collection | DOAJ |
| description | Immune disorder is considered the main pathogenesis of autoimmune diseases, such as rheumatoid arthritis (RA). The balance of the two special subsets of CD4+T cells, T helper cell 17 (Th17), and Regulator T cell (Treg) is the key factor of maintaining a normal immune response. Dendritic cells (DCs), which are the most powerful antigen-presenting cells, play an important role in regulating the balance of Th17 and Treg. The combination of disease modifying antirheumatic drugs (DMARDs) is an important strategy of RA therapy. In this study, we investigated the effect of MTX and CTX on DC maturation in ovalbumin (OVA) immunized mice. Th17 inflammatory response is stronger, while the level of DCs maturity is higher. In contrast, the immunosuppression of Treg is stronger. We found that MTX combined with CTX significantly inhibited the DCs maturity and downregulated the antigen presenting capacity of DCs. As a result, it reestablished a balance of Th17 and Treg. Our study adds a novel mechanism and therapeutic target of MTX combined with CTX for autoimmune disease treatment. |
| format | Article |
| id | doaj-art-af3a4fdf09af4bb880b1e89a61d2da88 |
| institution | OA Journals |
| issn | 1740-2522 1740-2530 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Clinical and Developmental Immunology |
| spelling | doaj-art-af3a4fdf09af4bb880b1e89a61d2da882025-08-20T02:19:06ZengWileyClinical and Developmental Immunology1740-25221740-25302013-01-01201310.1155/2013/238035238035Combination with Methotrexate and Cyclophosphamide Attenuated Maturation of Dendritic Cells: Inducing Treg Skewing and Th17 Suppression In VivoXiaoyang Yu0Caihong Wang1Jing Luo2Xiangcong Zhao3Lixing Wang4Xiaofeng Li5Department of Rheumatology, The Second Hospital of Shanxi Medical University, 382 Wu Yi Road, Taiyuan 030001, ChinaDepartment of Rheumatology, The Second Hospital of Shanxi Medical University, 382 Wu Yi Road, Taiyuan 030001, ChinaDepartment of Rheumatology, The Second Hospital of Shanxi Medical University, 382 Wu Yi Road, Taiyuan 030001, ChinaDepartment of Rheumatology, The Second Hospital of Shanxi Medical University, 382 Wu Yi Road, Taiyuan 030001, ChinaDepartment of Rheumatology, The Second Hospital of Shanxi Medical University, 382 Wu Yi Road, Taiyuan 030001, ChinaDepartment of Rheumatology, The Second Hospital of Shanxi Medical University, 382 Wu Yi Road, Taiyuan 030001, ChinaImmune disorder is considered the main pathogenesis of autoimmune diseases, such as rheumatoid arthritis (RA). The balance of the two special subsets of CD4+T cells, T helper cell 17 (Th17), and Regulator T cell (Treg) is the key factor of maintaining a normal immune response. Dendritic cells (DCs), which are the most powerful antigen-presenting cells, play an important role in regulating the balance of Th17 and Treg. The combination of disease modifying antirheumatic drugs (DMARDs) is an important strategy of RA therapy. In this study, we investigated the effect of MTX and CTX on DC maturation in ovalbumin (OVA) immunized mice. Th17 inflammatory response is stronger, while the level of DCs maturity is higher. In contrast, the immunosuppression of Treg is stronger. We found that MTX combined with CTX significantly inhibited the DCs maturity and downregulated the antigen presenting capacity of DCs. As a result, it reestablished a balance of Th17 and Treg. Our study adds a novel mechanism and therapeutic target of MTX combined with CTX for autoimmune disease treatment.http://dx.doi.org/10.1155/2013/238035 |
| spellingShingle | Xiaoyang Yu Caihong Wang Jing Luo Xiangcong Zhao Lixing Wang Xiaofeng Li Combination with Methotrexate and Cyclophosphamide Attenuated Maturation of Dendritic Cells: Inducing Treg Skewing and Th17 Suppression In Vivo Clinical and Developmental Immunology |
| title | Combination with Methotrexate and Cyclophosphamide Attenuated Maturation of Dendritic Cells: Inducing Treg Skewing and Th17 Suppression In Vivo |
| title_full | Combination with Methotrexate and Cyclophosphamide Attenuated Maturation of Dendritic Cells: Inducing Treg Skewing and Th17 Suppression In Vivo |
| title_fullStr | Combination with Methotrexate and Cyclophosphamide Attenuated Maturation of Dendritic Cells: Inducing Treg Skewing and Th17 Suppression In Vivo |
| title_full_unstemmed | Combination with Methotrexate and Cyclophosphamide Attenuated Maturation of Dendritic Cells: Inducing Treg Skewing and Th17 Suppression In Vivo |
| title_short | Combination with Methotrexate and Cyclophosphamide Attenuated Maturation of Dendritic Cells: Inducing Treg Skewing and Th17 Suppression In Vivo |
| title_sort | combination with methotrexate and cyclophosphamide attenuated maturation of dendritic cells inducing treg skewing and th17 suppression in vivo |
| url | http://dx.doi.org/10.1155/2013/238035 |
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