Protocol for recording neuronal activity in food and cocaine self-administration in freely behaving mice using calcium imaging
Summary: In vivo calcium imaging has revealed complex neural activity during operant conditioning. Here, we present a protocol for large-scale, long-term recording of neural activity in freely behaving mice using viral injection, gradient lens implantation, and miniscope imaging during food and coca...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-09-01
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| Series: | STAR Protocols |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166725003788 |
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| author | Jiale Wang Bin Wang Michael W. Wang Nicholas J. Beacher Da-Ting Lin Yan Zhang |
| author_facet | Jiale Wang Bin Wang Michael W. Wang Nicholas J. Beacher Da-Ting Lin Yan Zhang |
| author_sort | Jiale Wang |
| collection | DOAJ |
| description | Summary: In vivo calcium imaging has revealed complex neural activity during operant conditioning. Here, we present a protocol for large-scale, long-term recording of neural activity in freely behaving mice using viral injection, gradient lens implantation, and miniscope imaging during food and cocaine administration. We describe daily administration guidelines for comparing food- and cocaine-seeking behaviors. We further detail the procedures for the simultaneous self-administration of food and cocaine while integrating neural imaging recordings.For complete details on the use and execution of this protocol, please refer to Glanzberg et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| format | Article |
| id | doaj-art-af39eda063ee453bb35587093cb069bc |
| institution | Kabale University |
| issn | 2666-1667 |
| language | English |
| publishDate | 2025-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj-art-af39eda063ee453bb35587093cb069bc2025-08-20T03:51:49ZengElsevierSTAR Protocols2666-16672025-09-016310397210.1016/j.xpro.2025.103972Protocol for recording neuronal activity in food and cocaine self-administration in freely behaving mice using calcium imagingJiale Wang0Bin Wang1Michael W. Wang2Nicholas J. Beacher3Da-Ting Lin4Yan Zhang5National Institute on Drug Dependence and Beijing Key Laboratory of Drug Dependence Research, Peking University, Beijing 100191, China; School of Basic Medical Sciences, Peking University Health Science Center, Beijing 100191, ChinaNational Institute on Drug Dependence and Beijing Key Laboratory of Drug Dependence Research, Peking University, Beijing 100191, China; School of Basic Medical Sciences, Peking University Health Science Center, Beijing 100191, ChinaIntramural Research Program, National Institute on Drug Abuse, 333 Cassell Drive, Baltimore, MD 21224, USAIntramural Research Program, National Institute on Drug Abuse, 333 Cassell Drive, Baltimore, MD 21224, USAIntramural Research Program, National Institute on Drug Abuse, 333 Cassell Drive, Baltimore, MD 21224, USA; The Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, 725 N. Wolfe Street, Baltimore, MD 21205, USANational Institute on Drug Dependence and Beijing Key Laboratory of Drug Dependence Research, Peking University, Beijing 100191, China; School of Basic Medical Sciences, Peking University Health Science Center, Beijing 100191, China; Corresponding authorSummary: In vivo calcium imaging has revealed complex neural activity during operant conditioning. Here, we present a protocol for large-scale, long-term recording of neural activity in freely behaving mice using viral injection, gradient lens implantation, and miniscope imaging during food and cocaine administration. We describe daily administration guidelines for comparing food- and cocaine-seeking behaviors. We further detail the procedures for the simultaneous self-administration of food and cocaine while integrating neural imaging recordings.For complete details on the use and execution of this protocol, please refer to Glanzberg et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166725003788MicroscopyNeuroscienceCognitive NeuroscienceBehavior |
| spellingShingle | Jiale Wang Bin Wang Michael W. Wang Nicholas J. Beacher Da-Ting Lin Yan Zhang Protocol for recording neuronal activity in food and cocaine self-administration in freely behaving mice using calcium imaging STAR Protocols Microscopy Neuroscience Cognitive Neuroscience Behavior |
| title | Protocol for recording neuronal activity in food and cocaine self-administration in freely behaving mice using calcium imaging |
| title_full | Protocol for recording neuronal activity in food and cocaine self-administration in freely behaving mice using calcium imaging |
| title_fullStr | Protocol for recording neuronal activity in food and cocaine self-administration in freely behaving mice using calcium imaging |
| title_full_unstemmed | Protocol for recording neuronal activity in food and cocaine self-administration in freely behaving mice using calcium imaging |
| title_short | Protocol for recording neuronal activity in food and cocaine self-administration in freely behaving mice using calcium imaging |
| title_sort | protocol for recording neuronal activity in food and cocaine self administration in freely behaving mice using calcium imaging |
| topic | Microscopy Neuroscience Cognitive Neuroscience Behavior |
| url | http://www.sciencedirect.com/science/article/pii/S2666166725003788 |
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