Advantages of Syndromic Diagnostics: Detection of the Pathogens Causing Urethritis/Cervicitis with the STI CNM Real-Time PCR Kit from Vitro S.A.
The STI CNM Real-Time PCR Kit from Vitro S.A. (Spain) demonstrates high sensitivity and specificity, is cost-effective, and can detect the three main etiological agents of urethritis/cervicitis in a single multiplex PCR. Sexually transmitted infections (STIs) are a significant public health problem...
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| Language: | English |
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2024-12-01
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| Series: | Polish Journal of Microbiology |
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| Online Access: | https://doi.org/10.33073/pjm-2024-045 |
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| author | López Miguel Ángel Castaño Porteros Héctor Toledo De La Iglesia Salgado Alberto Medel Josefa Vazquez Borrero Inmaculada García |
| author_facet | López Miguel Ángel Castaño Porteros Héctor Toledo De La Iglesia Salgado Alberto Medel Josefa Vazquez Borrero Inmaculada García |
| author_sort | López Miguel Ángel Castaño |
| collection | DOAJ |
| description | The STI CNM Real-Time PCR Kit from Vitro S.A. (Spain) demonstrates high sensitivity and specificity, is cost-effective, and can detect the three main etiological agents of urethritis/cervicitis in a single multiplex PCR. Sexually transmitted infections (STIs) are a significant public health problem and a significant burden of morbidity and mortality in hospitals. The World Health Organization (WHO) estimates the number of daily infections to be 1 million. Currently, the number of infections and antimicrobial-resistant strains is rising. A rapid and accurate etiologic diagnosis is critical to solving this problem. In this study, we compared the STI CNM Real-Time PCR Kit using the Xpert® CT/NG technique (Cepheid®, USA) as Gold Standard for the diagnosis of Chlamydia trachomatis and Neisseria gonorrhoeae and EasyNAT® MG (Ustar Biotechnologies (Hangzhou) Ltd., China) as Gold Standard for the diagnosis of Mycoplasma genitalium infection. Regarding C. trachomatis and N. gonorrhoeae, out of 200 samples, there was a match in 199 cases, with only one positive sample not being detected by the STI CNM Real-Time PCR Kit. This results in a sensitivity of 96% and a specificity of 100% for this kit. Diagnosing M. genitalium infection, out of 200 samples, the STI CNM Real-Time PCR Kit correctly detected all negative and positive samples, with 100% agreement compared to the reference technique. In summary, the STI assay has a very high sensitivity and specificity, comparable to other commercial diagnostic kits. Furthermore, it has the advantage of bundling the detection of the three main bacterial agents of urethritis/cervicitis, resulting in better cost efficiency. |
| format | Article |
| id | doaj-art-af38aff5935d4352a747c1fdcf531ca9 |
| institution | DOAJ |
| issn | 2544-4646 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Polish Journal of Microbiology |
| spelling | doaj-art-af38aff5935d4352a747c1fdcf531ca92025-08-20T02:49:17ZengSciendoPolish Journal of Microbiology2544-46462024-12-0173452953410.33073/pjm-2024-045Advantages of Syndromic Diagnostics: Detection of the Pathogens Causing Urethritis/Cervicitis with the STI CNM Real-Time PCR Kit from Vitro S.A.López Miguel Ángel Castaño0Porteros Héctor Toledo1De La Iglesia Salgado Alberto2Medel Josefa Vazquez3Borrero Inmaculada García4Clinical Laboratory Department, Hospital Comarcal Infanta Elena, Huelva, SpainClinical Laboratory Department, Hospital Comarcal Infanta Elena, Huelva, SpainClinical Laboratory Department, Hospital Comarcal Infanta Elena, Huelva, SpainClinical Laboratory Department, Hospital Comarcal Infanta Elena, Huelva, SpainClinical Laboratory Department, Hospital Comarcal Infanta Elena, Huelva, SpainThe STI CNM Real-Time PCR Kit from Vitro S.A. (Spain) demonstrates high sensitivity and specificity, is cost-effective, and can detect the three main etiological agents of urethritis/cervicitis in a single multiplex PCR. Sexually transmitted infections (STIs) are a significant public health problem and a significant burden of morbidity and mortality in hospitals. The World Health Organization (WHO) estimates the number of daily infections to be 1 million. Currently, the number of infections and antimicrobial-resistant strains is rising. A rapid and accurate etiologic diagnosis is critical to solving this problem. In this study, we compared the STI CNM Real-Time PCR Kit using the Xpert® CT/NG technique (Cepheid®, USA) as Gold Standard for the diagnosis of Chlamydia trachomatis and Neisseria gonorrhoeae and EasyNAT® MG (Ustar Biotechnologies (Hangzhou) Ltd., China) as Gold Standard for the diagnosis of Mycoplasma genitalium infection. Regarding C. trachomatis and N. gonorrhoeae, out of 200 samples, there was a match in 199 cases, with only one positive sample not being detected by the STI CNM Real-Time PCR Kit. This results in a sensitivity of 96% and a specificity of 100% for this kit. Diagnosing M. genitalium infection, out of 200 samples, the STI CNM Real-Time PCR Kit correctly detected all negative and positive samples, with 100% agreement compared to the reference technique. In summary, the STI assay has a very high sensitivity and specificity, comparable to other commercial diagnostic kits. Furthermore, it has the advantage of bundling the detection of the three main bacterial agents of urethritis/cervicitis, resulting in better cost efficiency.https://doi.org/10.33073/pjm-2024-045stisyndromic diagnosticsmycoplasma genitaliumchlamydia trachomatisneisseria gonorrhoeae |
| spellingShingle | López Miguel Ángel Castaño Porteros Héctor Toledo De La Iglesia Salgado Alberto Medel Josefa Vazquez Borrero Inmaculada García Advantages of Syndromic Diagnostics: Detection of the Pathogens Causing Urethritis/Cervicitis with the STI CNM Real-Time PCR Kit from Vitro S.A. Polish Journal of Microbiology sti syndromic diagnostics mycoplasma genitalium chlamydia trachomatis neisseria gonorrhoeae |
| title | Advantages of Syndromic Diagnostics: Detection of the Pathogens Causing Urethritis/Cervicitis with the STI CNM Real-Time PCR Kit from Vitro S.A. |
| title_full | Advantages of Syndromic Diagnostics: Detection of the Pathogens Causing Urethritis/Cervicitis with the STI CNM Real-Time PCR Kit from Vitro S.A. |
| title_fullStr | Advantages of Syndromic Diagnostics: Detection of the Pathogens Causing Urethritis/Cervicitis with the STI CNM Real-Time PCR Kit from Vitro S.A. |
| title_full_unstemmed | Advantages of Syndromic Diagnostics: Detection of the Pathogens Causing Urethritis/Cervicitis with the STI CNM Real-Time PCR Kit from Vitro S.A. |
| title_short | Advantages of Syndromic Diagnostics: Detection of the Pathogens Causing Urethritis/Cervicitis with the STI CNM Real-Time PCR Kit from Vitro S.A. |
| title_sort | advantages of syndromic diagnostics detection of the pathogens causing urethritis cervicitis with the sti cnm real time pcr kit from vitro s a |
| topic | sti syndromic diagnostics mycoplasma genitalium chlamydia trachomatis neisseria gonorrhoeae |
| url | https://doi.org/10.33073/pjm-2024-045 |
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