Intrinsic factors influence a physiological measure in a forest bird community: adults and females have higher H/L ratios than juveniles and males

Physiological parameters have the potential to serve as valuable early warning indicators for the conservation of animal populations. However, measuring physiological adaptations in wildlife is often challenging, due to intrinsic differences causing natural variations of physiological measures betwe...

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Main Authors: Finja Strehmann, Markus Vogelbacher, Clara Guckenbiehl, Yvonne R. Schumm, Juan F. Masello, Petra Quillfeldt, Nikolaus Korfhage, Hicham Bellafkir, Markus Mühling, Bernd Freisleben, Nina Farwig, Dana G. Schabo, Sascha Rösner
Format: Article
Language:English
Published: Wiley 2025-03-01
Series:Journal of Avian Biology
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Online Access:https://doi.org/10.1111/jav.03269
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author Finja Strehmann
Markus Vogelbacher
Clara Guckenbiehl
Yvonne R. Schumm
Juan F. Masello
Petra Quillfeldt
Nikolaus Korfhage
Hicham Bellafkir
Markus Mühling
Bernd Freisleben
Nina Farwig
Dana G. Schabo
Sascha Rösner
author_facet Finja Strehmann
Markus Vogelbacher
Clara Guckenbiehl
Yvonne R. Schumm
Juan F. Masello
Petra Quillfeldt
Nikolaus Korfhage
Hicham Bellafkir
Markus Mühling
Bernd Freisleben
Nina Farwig
Dana G. Schabo
Sascha Rösner
author_sort Finja Strehmann
collection DOAJ
description Physiological parameters have the potential to serve as valuable early warning indicators for the conservation of animal populations. However, measuring physiological adaptations in wildlife is often challenging, due to intrinsic differences causing natural variations of physiological measures between individuals across species. This study is aimed at addressing this by investigating the influence of intrinsic factors, including sex, age, body condition, and the incubation of eggs on the H/L ratio of a forest bird community. As physiological measure, we used the heterophil to lymphocyte (H/L) ratio of individuals belonging to different species in the forest bird community, which was assessed using a novel deep learning approach based on convolutional neural networks (CNNs) applied to whole blood smear scans. Using phylogenetically controlled Bayesian analyses across the bird species, we found higher H/L ratios in adult birds than in juveniles and observed slightly higher H/L ratios in females than in males. While body condition had no effect on the H/L ratio, incubating birds tended to have higher H/L ratios than non‐reproductive birds, regardless of their sex. Furthermore, we found a robust phylogenetic signal of the H/L ratio in the studied bird community. Our results reveal significant general patterns of the effect of intrinsic factors on the H/L ratio across a bird community.
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spelling doaj-art-af29d2b714ad43399c7f55a91fddac382025-08-20T02:29:39ZengWileyJournal of Avian Biology0908-88571600-048X2025-03-0120252n/an/a10.1111/jav.03269Intrinsic factors influence a physiological measure in a forest bird community: adults and females have higher H/L ratios than juveniles and malesFinja Strehmann0Markus Vogelbacher1Clara Guckenbiehl2Yvonne R. Schumm3Juan F. Masello4Petra Quillfeldt5Nikolaus Korfhage6Hicham Bellafkir7Markus Mühling8Bernd Freisleben9Nina Farwig10Dana G. Schabo11Sascha Rösner12Conservation Ecology, Department of Biology, Philipps‐Universität Marburg Marburg GermanyDepartment of Mathematics and Computer Science, Philipps‐Universität Marburg Marburg GermanyConservation Ecology, Department of Biology, Philipps‐Universität Marburg Marburg GermanyDepartment of Animal Ecology and Systematics, Behavioural Ecology and Ecophysiology Group, Justus‐Liebig‐Universität Giessen Giessen GermanyDepartment of Evolutionary Population Genetics, Bielefeld University Bielefeld GermanyDepartment of Animal Ecology and Systematics, Behavioural Ecology and Ecophysiology Group, Justus‐Liebig‐Universität Giessen Giessen GermanyDepartment of Mathematics and Computer Science, Philipps‐Universität Marburg Marburg GermanyDepartment of Mathematics and Computer Science, Philipps‐Universität Marburg Marburg GermanyDepartment of Mathematics and Computer Science, Philipps‐Universität Marburg Marburg GermanyDepartment of Mathematics and Computer Science, Philipps‐Universität Marburg Marburg GermanyConservation Ecology, Department of Biology, Philipps‐Universität Marburg Marburg GermanyConservation Ecology, Department of Biology, Philipps‐Universität Marburg Marburg GermanyConservation Ecology, Department of Biology, Philipps‐Universität Marburg Marburg GermanyPhysiological parameters have the potential to serve as valuable early warning indicators for the conservation of animal populations. However, measuring physiological adaptations in wildlife is often challenging, due to intrinsic differences causing natural variations of physiological measures between individuals across species. This study is aimed at addressing this by investigating the influence of intrinsic factors, including sex, age, body condition, and the incubation of eggs on the H/L ratio of a forest bird community. As physiological measure, we used the heterophil to lymphocyte (H/L) ratio of individuals belonging to different species in the forest bird community, which was assessed using a novel deep learning approach based on convolutional neural networks (CNNs) applied to whole blood smear scans. Using phylogenetically controlled Bayesian analyses across the bird species, we found higher H/L ratios in adult birds than in juveniles and observed slightly higher H/L ratios in females than in males. While body condition had no effect on the H/L ratio, incubating birds tended to have higher H/L ratios than non‐reproductive birds, regardless of their sex. Furthermore, we found a robust phylogenetic signal of the H/L ratio in the studied bird community. Our results reveal significant general patterns of the effect of intrinsic factors on the H/L ratio across a bird community.https://doi.org/10.1111/jav.03269conservation physiologyhematologyleukocyte countsmachine learningstress index
spellingShingle Finja Strehmann
Markus Vogelbacher
Clara Guckenbiehl
Yvonne R. Schumm
Juan F. Masello
Petra Quillfeldt
Nikolaus Korfhage
Hicham Bellafkir
Markus Mühling
Bernd Freisleben
Nina Farwig
Dana G. Schabo
Sascha Rösner
Intrinsic factors influence a physiological measure in a forest bird community: adults and females have higher H/L ratios than juveniles and males
Journal of Avian Biology
conservation physiology
hematology
leukocyte counts
machine learning
stress index
title Intrinsic factors influence a physiological measure in a forest bird community: adults and females have higher H/L ratios than juveniles and males
title_full Intrinsic factors influence a physiological measure in a forest bird community: adults and females have higher H/L ratios than juveniles and males
title_fullStr Intrinsic factors influence a physiological measure in a forest bird community: adults and females have higher H/L ratios than juveniles and males
title_full_unstemmed Intrinsic factors influence a physiological measure in a forest bird community: adults and females have higher H/L ratios than juveniles and males
title_short Intrinsic factors influence a physiological measure in a forest bird community: adults and females have higher H/L ratios than juveniles and males
title_sort intrinsic factors influence a physiological measure in a forest bird community adults and females have higher h l ratios than juveniles and males
topic conservation physiology
hematology
leukocyte counts
machine learning
stress index
url https://doi.org/10.1111/jav.03269
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