Receptor binding and structural basis of raccoon dog ACE2 binding to SARS-CoV-2 prototype and its variants.
Raccoon dog was proposed as a potential host of SARS-CoV-2, but no evidence support such a notion. In our study, we investigated the binding affinities of raccoon dog ACE2 (rdACE2) to the spike (S) protein receptor binding domain (RBD) of SARS-CoV-2 prototype (PT) and its variants. It revealed that...
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| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2024-12-01
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| Series: | PLoS Pathogens |
| Online Access: | https://journals.plos.org/plospathogens/article/file?id=10.1371/journal.ppat.1012713&type=printable |
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| author | Chunliang Luo Linjie Li Yuhang Gu Hangchuan Zhang Zepeng Xu Junqing Sun Kaiyuan Shi Sufang Ma Wen-Xia Tian Kefang Liu George F Gao |
| author_facet | Chunliang Luo Linjie Li Yuhang Gu Hangchuan Zhang Zepeng Xu Junqing Sun Kaiyuan Shi Sufang Ma Wen-Xia Tian Kefang Liu George F Gao |
| author_sort | Chunliang Luo |
| collection | DOAJ |
| description | Raccoon dog was proposed as a potential host of SARS-CoV-2, but no evidence support such a notion. In our study, we investigated the binding affinities of raccoon dog ACE2 (rdACE2) to the spike (S) protein receptor binding domain (RBD) of SARS-CoV-2 prototype (PT) and its variants. It revealed that the binding affinities of RBD from SARS-CoV-2 variants were generally lower than that of the PT RBD. Through structural and functional analyses, we found amino acids H34 and M82 play pivotal roles in maintaining the binding affinity of ACE2 to different SARS-CoV-2 sub-variants. These results suggest that raccoon dogs exhibit lower susceptibility to SARS-CoV-2 compared to those animal species with a high prevalence of SARS-CoV-2 transmission. |
| format | Article |
| id | doaj-art-af28007fee0c4d869d4487cc22e0b39c |
| institution | OA Journals |
| issn | 1553-7366 1553-7374 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Pathogens |
| spelling | doaj-art-af28007fee0c4d869d4487cc22e0b39c2025-08-20T02:38:58ZengPublic Library of Science (PLoS)PLoS Pathogens1553-73661553-73742024-12-012012e101271310.1371/journal.ppat.1012713Receptor binding and structural basis of raccoon dog ACE2 binding to SARS-CoV-2 prototype and its variants.Chunliang LuoLinjie LiYuhang GuHangchuan ZhangZepeng XuJunqing SunKaiyuan ShiSufang MaWen-Xia TianKefang LiuGeorge F GaoRaccoon dog was proposed as a potential host of SARS-CoV-2, but no evidence support such a notion. In our study, we investigated the binding affinities of raccoon dog ACE2 (rdACE2) to the spike (S) protein receptor binding domain (RBD) of SARS-CoV-2 prototype (PT) and its variants. It revealed that the binding affinities of RBD from SARS-CoV-2 variants were generally lower than that of the PT RBD. Through structural and functional analyses, we found amino acids H34 and M82 play pivotal roles in maintaining the binding affinity of ACE2 to different SARS-CoV-2 sub-variants. These results suggest that raccoon dogs exhibit lower susceptibility to SARS-CoV-2 compared to those animal species with a high prevalence of SARS-CoV-2 transmission.https://journals.plos.org/plospathogens/article/file?id=10.1371/journal.ppat.1012713&type=printable |
| spellingShingle | Chunliang Luo Linjie Li Yuhang Gu Hangchuan Zhang Zepeng Xu Junqing Sun Kaiyuan Shi Sufang Ma Wen-Xia Tian Kefang Liu George F Gao Receptor binding and structural basis of raccoon dog ACE2 binding to SARS-CoV-2 prototype and its variants. PLoS Pathogens |
| title | Receptor binding and structural basis of raccoon dog ACE2 binding to SARS-CoV-2 prototype and its variants. |
| title_full | Receptor binding and structural basis of raccoon dog ACE2 binding to SARS-CoV-2 prototype and its variants. |
| title_fullStr | Receptor binding and structural basis of raccoon dog ACE2 binding to SARS-CoV-2 prototype and its variants. |
| title_full_unstemmed | Receptor binding and structural basis of raccoon dog ACE2 binding to SARS-CoV-2 prototype and its variants. |
| title_short | Receptor binding and structural basis of raccoon dog ACE2 binding to SARS-CoV-2 prototype and its variants. |
| title_sort | receptor binding and structural basis of raccoon dog ace2 binding to sars cov 2 prototype and its variants |
| url | https://journals.plos.org/plospathogens/article/file?id=10.1371/journal.ppat.1012713&type=printable |
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