Single-Cell RNA-Seq Uncovers Cellular Heterogeneity from Deep Fascia in Necrotizing Fasciitis Patients

Tao Wang,1,* Liping Zhang,2,* Wei Chen,3 Yubin Long,3 Yingze Zhang,3– 6 Ling Wang,3 Zhiyong Hou3– 6 1Department of Lower Limb Trauma, Beijing Jishuitan Hospital, Guizhou Hospital, Guiyang, Guizhou, People’s Republic of China; 2Department of Physiology, Hebei Medical University, Shiji...

Full description

Saved in:
Bibliographic Details
Main Authors: Wang T, Zhang L, Chen W, Long Y, Zhang Y, Wang L, Hou Z
Format: Article
Language:English
Published: Dove Medical Press 2025-01-01
Series:Journal of Inflammation Research
Subjects:
Online Access:https://www.dovepress.com/single-cell-rna-seq-uncovers-cellular-heterogeneity-from-deep-fascia-i-peer-reviewed-fulltext-article-JIR
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1832592083109543936
author Wang T
Zhang L
Chen W
Long Y
Zhang Y
Wang L
Hou Z
author_facet Wang T
Zhang L
Chen W
Long Y
Zhang Y
Wang L
Hou Z
author_sort Wang T
collection DOAJ
description Tao Wang,1,* Liping Zhang,2,* Wei Chen,3 Yubin Long,3 Yingze Zhang,3– 6 Ling Wang,3 Zhiyong Hou3– 6 1Department of Lower Limb Trauma, Beijing Jishuitan Hospital, Guizhou Hospital, Guiyang, Guizhou, People’s Republic of China; 2Department of Physiology, Hebei Medical University, Shijiazhuang, Hebei, People’s Republic of China; 3Department of Orthopaedic Surgery, Third Hospital of Hebei Medical University, Shijiazhuang, Hebei, People’s Republic of China; 4Engineering Research Center of Orthopedic Minimally Invasive Intelligent Equipment, Ministry of Education, Shanghai, People’s Republic of China; 5Key Laboratory of Biomechanics of Hebei Province, Shijiazhuang, Hebei, People’s Republic of China; 6NHC Key Laboratory of Intelligent Orthopaedic Equipment, Shijiazhuang, Hebei, People’s Republic of China*These authors contributed equally to this workCorrespondence: Zhiyong Hou, Department of Orthopaedic Surgery, Third Hospital of Hebei Medical University, Shijiazhuang, Hebei, P.R. China. No. 139 Road Ziqiang, Shijiazhuang, Hebei, People’s Republic of China, Email drzyhou@gmail.comPurpose: Necrotizing fasciitis (NF) is a scarce but potentially life-threatening infection. However, no research has reported the cellular heterogeneity in patients with NF. We aim to investigate the change of cells from deep fascia in response to NF by single-cell RNA-seq.Methods: Fascia samples from NF patients (NF group, NG, n = 3) and volunteer (control group, CG, n = 4) were obtained and we utilized scRNA-seq to observe the variation of cells and differentially expressed genes. Then, multiplex staining and multispectral imaging and immunohistochemistry were used to be further verified.Results: Our findings showed that three fibroblast subclusters (antigen-presenting Fib, mesenchymal Fib, and myoFib) and three macrophage subclusters (SPP1+ Mac0, IL1B+ Mac1, and SPP1+M2) were found to have increased proportions with distinct roles in NF patients. The balance of M1/M2 polarization may be the key therapeutic target to determine the outcome of NF. Furthermore, the levels of SAA1, PTX3, S100 family, MARCO, and STAB1 were up-regulated in different subclusters with anti-infection roles against NF, which were proven by immunohistochemistry. These proteins may act as a biomarker or even as a candidate therapy for NF.Conclusion: Our findings revealed the potential anti-infection role of deep fascia during the procession of NF, helping us understand the immunologic function of fascia and provide novel insights for its therapeutic strategies for NF.Keywords: necrotizing fasciitis, deep fascia, single-cell RNA-seq, M1/M2 polarization, fibroblast
format Article
id doaj-art-af257bdeb5144be390512a50003dc716
institution Kabale University
issn 1178-7031
language English
publishDate 2025-01-01
publisher Dove Medical Press
record_format Article
series Journal of Inflammation Research
spelling doaj-art-af257bdeb5144be390512a50003dc7162025-01-21T16:58:06ZengDove Medical PressJournal of Inflammation Research1178-70312025-01-01Volume 18995101299459Single-Cell RNA-Seq Uncovers Cellular Heterogeneity from Deep Fascia in Necrotizing Fasciitis PatientsWang TZhang LChen WLong YZhang YWang LHou ZTao Wang,1,* Liping Zhang,2,* Wei Chen,3 Yubin Long,3 Yingze Zhang,3– 6 Ling Wang,3 Zhiyong Hou3– 6 1Department of Lower Limb Trauma, Beijing Jishuitan Hospital, Guizhou Hospital, Guiyang, Guizhou, People’s Republic of China; 2Department of Physiology, Hebei Medical University, Shijiazhuang, Hebei, People’s Republic of China; 3Department of Orthopaedic Surgery, Third Hospital of Hebei Medical University, Shijiazhuang, Hebei, People’s Republic of China; 4Engineering Research Center of Orthopedic Minimally Invasive Intelligent Equipment, Ministry of Education, Shanghai, People’s Republic of China; 5Key Laboratory of Biomechanics of Hebei Province, Shijiazhuang, Hebei, People’s Republic of China; 6NHC Key Laboratory of Intelligent Orthopaedic Equipment, Shijiazhuang, Hebei, People’s Republic of China*These authors contributed equally to this workCorrespondence: Zhiyong Hou, Department of Orthopaedic Surgery, Third Hospital of Hebei Medical University, Shijiazhuang, Hebei, P.R. China. No. 139 Road Ziqiang, Shijiazhuang, Hebei, People’s Republic of China, Email drzyhou@gmail.comPurpose: Necrotizing fasciitis (NF) is a scarce but potentially life-threatening infection. However, no research has reported the cellular heterogeneity in patients with NF. We aim to investigate the change of cells from deep fascia in response to NF by single-cell RNA-seq.Methods: Fascia samples from NF patients (NF group, NG, n = 3) and volunteer (control group, CG, n = 4) were obtained and we utilized scRNA-seq to observe the variation of cells and differentially expressed genes. Then, multiplex staining and multispectral imaging and immunohistochemistry were used to be further verified.Results: Our findings showed that three fibroblast subclusters (antigen-presenting Fib, mesenchymal Fib, and myoFib) and three macrophage subclusters (SPP1+ Mac0, IL1B+ Mac1, and SPP1+M2) were found to have increased proportions with distinct roles in NF patients. The balance of M1/M2 polarization may be the key therapeutic target to determine the outcome of NF. Furthermore, the levels of SAA1, PTX3, S100 family, MARCO, and STAB1 were up-regulated in different subclusters with anti-infection roles against NF, which were proven by immunohistochemistry. These proteins may act as a biomarker or even as a candidate therapy for NF.Conclusion: Our findings revealed the potential anti-infection role of deep fascia during the procession of NF, helping us understand the immunologic function of fascia and provide novel insights for its therapeutic strategies for NF.Keywords: necrotizing fasciitis, deep fascia, single-cell RNA-seq, M1/M2 polarization, fibroblasthttps://www.dovepress.com/single-cell-rna-seq-uncovers-cellular-heterogeneity-from-deep-fascia-i-peer-reviewed-fulltext-article-JIRnecrotizing fasciitisdeep fasciasingle-cell rna-seqm1/m2 polarizationfibroblast
spellingShingle Wang T
Zhang L
Chen W
Long Y
Zhang Y
Wang L
Hou Z
Single-Cell RNA-Seq Uncovers Cellular Heterogeneity from Deep Fascia in Necrotizing Fasciitis Patients
Journal of Inflammation Research
necrotizing fasciitis
deep fascia
single-cell rna-seq
m1/m2 polarization
fibroblast
title Single-Cell RNA-Seq Uncovers Cellular Heterogeneity from Deep Fascia in Necrotizing Fasciitis Patients
title_full Single-Cell RNA-Seq Uncovers Cellular Heterogeneity from Deep Fascia in Necrotizing Fasciitis Patients
title_fullStr Single-Cell RNA-Seq Uncovers Cellular Heterogeneity from Deep Fascia in Necrotizing Fasciitis Patients
title_full_unstemmed Single-Cell RNA-Seq Uncovers Cellular Heterogeneity from Deep Fascia in Necrotizing Fasciitis Patients
title_short Single-Cell RNA-Seq Uncovers Cellular Heterogeneity from Deep Fascia in Necrotizing Fasciitis Patients
title_sort single cell rna seq uncovers cellular heterogeneity from deep fascia in necrotizing fasciitis patients
topic necrotizing fasciitis
deep fascia
single-cell rna-seq
m1/m2 polarization
fibroblast
url https://www.dovepress.com/single-cell-rna-seq-uncovers-cellular-heterogeneity-from-deep-fascia-i-peer-reviewed-fulltext-article-JIR
work_keys_str_mv AT wangt singlecellrnasequncoverscellularheterogeneityfromdeepfasciainnecrotizingfasciitispatients
AT zhangl singlecellrnasequncoverscellularheterogeneityfromdeepfasciainnecrotizingfasciitispatients
AT chenw singlecellrnasequncoverscellularheterogeneityfromdeepfasciainnecrotizingfasciitispatients
AT longy singlecellrnasequncoverscellularheterogeneityfromdeepfasciainnecrotizingfasciitispatients
AT zhangy singlecellrnasequncoverscellularheterogeneityfromdeepfasciainnecrotizingfasciitispatients
AT wangl singlecellrnasequncoverscellularheterogeneityfromdeepfasciainnecrotizingfasciitispatients
AT houz singlecellrnasequncoverscellularheterogeneityfromdeepfasciainnecrotizingfasciitispatients